nucleotide-excision repair factor 4 complex

GO:0000113 · Other · Multiprotein complex · Proposed

One of several protein complexes involved in nucleotide-excision repair; possesses DNA damage recognition and DNA-dependent ATPase activities. In S. cerevisiae, it is composed of Rad7p and Rad16p. [GO:0000113]

Synonyms: NEF4 complex

UniProt location names: UniProt Consortium, CC BY 4.0. Reference label and route sources.

Components

Component	Type	Part of / acts on	Grounding	Genes	Stoichiometry	Essential	Role
Rad7 NEF4 subunit	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	RAD7		UNKNOWN	Protein constituent of the budding-yeast nucleotide-excision repair factor 4 / NEF4 complex. `GO:0000113` GO:0000113 defines the NEF4 complex as containing Rad7p.
Rad16 NEF4 subunit	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	RAD16		UNKNOWN	Protein constituent of the budding-yeast nucleotide-excision repair factor 4 / NEF4 complex. `GO:0000113` GO:0000113 defines the NEF4 complex as containing Rad16p.

Taxonomic distribution

NCBITaxon:4932 Saccharomyces cerevisiae — COMMON: Budding yeast is the organism in which GO:0000113 defines NEF4 as a Rad7p/Rad16p nucleotide-excision repair factor complex. [GO:0000113]

Canonical examples

NCBITaxon:4932 Saccharomyces cerevisiae — Prakash and Prakash 2000 described the budding-yeast Rad7-Rad16 complex as the nucleotide-excision repair subassembly called NEF4. [DOI:10.1016/s0027-5107(00)00037-3]

Functions

nucleotide-excision repair GO:0006289 — The NEF4 complex participates in nucleotide-excision repair as a DNA-damage-recognition and DNA-dependent-ATPase-containing protein complex.
- GO:0000113 GO:0000113 defines NEF4 as a nucleotide-excision repair factor with DNA damage recognition and DNA-dependent ATPase activities.
- GO:0006289 GO:0006289 is the Gene Ontology biological-process term for nucleotide-excision repair.
- DOI:10.1016/s0027-5107(00)00037-3 Prakash and Prakash 2000 described the Rad7-Rad16 complex as the budding-yeast NEF4 multiprotein subassembly.

Mechanism graphs

NEF4 is a yeast nucleotide-excision repair factor (FUNCTION)

In Saccharomyces cerevisiae, Rad7p and Rad16p form the NEF4 subassembly of the nucleotide-excision repair protein system.

Subject	Predicate	Object	Evidence
Rad7 NEF4 subunit	contributes to	NEF4 complex	`GO:0000113` GO:0000113 defines Rad7p as a component of the NEF4 complex.
Rad16 NEF4 subunit	contributes to	NEF4 complex	`GO:0000113` GO:0000113 defines Rad16p as a component of the NEF4 complex.
NEF4 complex	supports	nucleotide-excision repair	`GO:0000113` GO:0000113 defines NEF4 as one of several complexes involved in nucleotide-excision repair. `GO:0006289` GO:0006289 is the process term for nucleotide-excision repair. `DOI:10.1016/s0027-5107(00)00037-3` Prakash and Prakash 2000 described NEF4 as an in-vivo multiprotein subassembly of the yeast NER proteins.

Discussions

CURATION_TODO (OPEN): Resolve exact source-neutral family groundings for the Rad7 and Rad16 constituents of the NEF4 complex. GO:0000113 defines budding-yeast Rad7p and Rad16p as the NEF4 constituents. Exact cross-eukaryotic InterPro, Pfam, NCBIfam or UniProt family boundaries for these NER factor classes need narrower curation before these component labels can be grounded.

Evidence

GO:0000113 Gene Ontology cellular-component term for the NEF4 complex and its exact NEF4 synonym.
GO:0000109 Gene Ontology cellular-component parent term for nucleotide-excision repair complexes.
GO:0006289 Gene Ontology biological-process term for nucleotide-excision repair.
PMID:10915862 PubMed identifier for Prakash and Prakash 2000, the Mutation Research yeast nucleotide-excision repair review cited by GO:0000113.
DOI:10.1016/s0027-5107(00)00037-3 DOI for Prakash and Prakash 2000, 'Nucleotide excision repair in yeast', verified through NCBI E-utilities.

Curation history

2026-09-26T03:00:07Z codex · CREATED_RECORD — Scaffolded by scripts/new_record.py
2026-09-26T03:00:54Z codex · EXPANDED_RECORD — Added NEF4 exact synonymy, nucleotide-excision repair complex parentage, two reviewed-label-only Rad constituent rows, budding-yeast scope, a nucleotide-excision-repair function, GO/PubMed/DOI evidence, a nonmechanistic function graph, and a NEF4 subunit-grounding TODO.

Source: data/structures/other/nucleotide_excision_repair_factor_4_complex.yaml