Rad17 RFC-like complex

GO:0031389 · Other · Multiprotein complex · Proposed

A pentameric protein complex related to replication factor C that loads the checkpoint clamp onto DNA at damage sites and functions in DNA damage cell-cycle checkpoints; the large subunit is Rad17 in Schizosaccharomyces pombe and Rad24p in Saccharomyces cerevisiae. [GO:0031389]

Synonyms: Rad17-RFC; Rad17-RLC; Rad24p RFC-like complex; RFC (Rad17)

UniProt location names: UniProt Consortium, CC BY 4.0. Reference label and route sources.

Components

Component	Type	Part of / acts on	Grounding	Genes	Essential	Role
Rad24 subunit	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	RAD24	UNKNOWN	Named Saccharomyces cerevisiae constituent of the pentameric Rad17/Rad24 RFC-like complex. `GO:0031389` GO:0031389 defines the Saccharomyces cerevisiae Rad17/Rad24 RFC-like complex as containing Rad24p, Rfc2p, Rfc3p, Rfc4p and Rfc5p.
Rfc2 subunit	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	RFC2	UNKNOWN	Named Saccharomyces cerevisiae constituent of the pentameric Rad17/Rad24 RFC-like complex. `GO:0031389` GO:0031389 defines the Saccharomyces cerevisiae Rad17/Rad24 RFC-like complex as containing Rad24p, Rfc2p, Rfc3p, Rfc4p and Rfc5p.
Rfc3 subunit	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	RFC3	UNKNOWN	Named Saccharomyces cerevisiae constituent of the pentameric Rad17/Rad24 RFC-like complex. `GO:0031389` GO:0031389 defines the Saccharomyces cerevisiae Rad17/Rad24 RFC-like complex as containing Rad24p, Rfc2p, Rfc3p, Rfc4p and Rfc5p.
Rfc4 subunit	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	RFC4	UNKNOWN	Named Saccharomyces cerevisiae constituent of the pentameric Rad17/Rad24 RFC-like complex. `GO:0031389` GO:0031389 defines the Saccharomyces cerevisiae Rad17/Rad24 RFC-like complex as containing Rad24p, Rfc2p, Rfc3p, Rfc4p and Rfc5p.
Rfc5 subunit	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	RFC5	UNKNOWN	Named Saccharomyces cerevisiae constituent of the pentameric Rad17/Rad24 RFC-like complex. `GO:0031389` GO:0031389 defines the Saccharomyces cerevisiae Rad17/Rad24 RFC-like complex as containing Rad24p, Rfc2p, Rfc3p, Rfc4p and Rfc5p.

Taxonomic distribution

NCBITaxon:4932 Saccharomyces cerevisiae — COMMON: Budding yeast has a Rad17/Rad24 RFC-like complex containing Rad24p, Rfc2p, Rfc3p, Rfc4p and Rfc5p. [GO:0031389]

Canonical examples

NCBITaxon:4932 Saccharomyces cerevisiae — Reference budding-yeast system named in the GO:0031389 definition as containing Rad24p and the four small RFC subunits. [GO:0031389]

Functions

DNA clamp loader activity GO:0003689 — The Rad17/Rad24 RFC-like complex loads the checkpoint clamp onto DNA at damage sites.
- GO:0031389 GO:0031389 defines the Rad17 RFC-like complex as loading the checkpoint clamp onto DNA at damage sites.
- GO:0003689 GO:0003689 is the Gene Ontology molecular-function term for DNA clamp loader activity.
DNA damage response GO:0006974 — The Rad17/Rad24 RFC-like complex supports DNA damage checkpoint responses.
- GO:0031389 GO:0031389 defines the Rad17 RFC-like complex as functioning in DNA damage cell cycle checkpoints.
- GO:0006974 GO:0006974 is the Gene Ontology biological-process term for DNA damage response.

Mechanism graphs

Rad17/Rad24-RFC loads the checkpoint clamp (FUNCTION)

Rad24p, Rfc2p, Rfc3p, Rfc4p and Rfc5p form the budding-yeast Rad17/Rad24 RFC-like complex that loads the checkpoint clamp onto damaged DNA and supports DNA damage checkpoint responses.

Subject	Predicate	Object	Evidence
Rad24 subunit	contributes to	Rad17 RFC-like complex	`GO:0031389` GO:0031389 defines the Saccharomyces cerevisiae Rad17/Rad24 RFC-like complex as containing Rad24p, Rfc2p, Rfc3p, Rfc4p and Rfc5p.
Rfc2 subunit	contributes to	Rad17 RFC-like complex	`GO:0031389` GO:0031389 defines the Saccharomyces cerevisiae Rad17/Rad24 RFC-like complex as containing Rad24p, Rfc2p, Rfc3p, Rfc4p and Rfc5p.
Rfc3 subunit	contributes to	Rad17 RFC-like complex	`GO:0031389` GO:0031389 defines the Saccharomyces cerevisiae Rad17/Rad24 RFC-like complex as containing Rad24p, Rfc2p, Rfc3p, Rfc4p and Rfc5p.
Rfc4 subunit	contributes to	Rad17 RFC-like complex	`GO:0031389` GO:0031389 defines the Saccharomyces cerevisiae Rad17/Rad24 RFC-like complex as containing Rad24p, Rfc2p, Rfc3p, Rfc4p and Rfc5p.
Rfc5 subunit	contributes to	Rad17 RFC-like complex	`GO:0031389` GO:0031389 defines the Saccharomyces cerevisiae Rad17/Rad24 RFC-like complex as containing Rad24p, Rfc2p, Rfc3p, Rfc4p and Rfc5p.
Rad17 RFC-like complex	loads	checkpoint clamp complex	`GO:0031389` GO:0031389 defines the Rad17 RFC-like complex as loading the checkpoint clamp onto DNA at damage sites.
Rad17 RFC-like complex	supports	DNA damage response	`GO:0031389` GO:0031389 defines the Rad17 RFC-like complex as functioning in DNA damage cell cycle checkpoints. `GO:0006974` GO:0006974 is the biological-process term for DNA damage response.

Discussions

CURATION_TODO (OPEN): Resolve exact source-neutral family groundings for the Rad24, Rfc2, Rfc3, Rfc4 and Rfc5 constituents of the Rad17 RFC-like complex. GO:0031389 exactly identifies the Rad17/Rad24 RFC-like complex and names the budding-yeast Rad24p, Rfc2p, Rfc3p, Rfc4p and Rfc5p constituents, but exact source-neutral InterPro, Pfam, NCBIfam or UniProtKB family CURIEs for these subunit classes were not curated in this pass.

Evidence

GO:0031389 Gene Ontology cellular-component term for the Rad17 RFC-like complex.
GO:0032991 Gene Ontology cellular-component parent term for protein-containing complexes.
GO:0003689 Gene Ontology molecular-function term for DNA clamp loader activity.
GO:0006974 Gene Ontology biological-process term for DNA damage response.
PMID:14614842 PubMed identifier for the review listed as the GO:0031389 definition source.

Curation history

2026-09-27T15:11:21Z codex · CREATED_RECORD — Scaffolded by scripts/new_record.py
2026-09-27T15:12:53Z codex · EXPANDED_RECORD — Added GO Rad17/Rad24-RFC synonymy, protein-containing-complex parentage, five reviewed-label-only budding-yeast constituent rows, Saccharomyces cerevisiae scope, checkpoint-clamp loader and DNA-damage-response function evidence, GO/PubMed evidence, a nonmechanistic function graph, and a Rad17/Rad24-RFC subunit-grounding TODO.
2026-09-27T15:25:38Z codex · TIGHTEN_GRAPH — Removed the damaged DNA node and checkpoint-clamp-to-damaged-DNA graph edge because GO:0031389 supports Rad17/Rad24-RFC loading the checkpoint clamp at DNA damage sites, not a separate checkpoint clamp self-loading edge. Addresses issue #1664.

Source: data/structures/other/rad17_rfc_like_complex.yaml