Ragulator complex

GO:0071986 · Other · Multiprotein complex · Proposed

A vacuolar membrane-anchored guanine nucleotide exchange factor (GEF) complex for the Rag GTPases (Gtr1-Gtr2 GTPase complex GO:1990131) in TORC1 signaling pathway. In human, Ragulator is comprised of the membrane anchor subunit LAMTOR1 (Meh1p in S. cerevisiae, Lam1 in S. pombe), a GEF subunit LAMTOR2 ( Slm4 in S. cerevisiae , Lam2 in S. pombe ) , LAMTOR3 (no S. cerevisiae ortholog identified, Lam3 in S. pombe) , LAMTOR4 (no S. cerevisiae ortholog identified, Lam4 in S. pombe), and LAMTOR5 (no S. cerevisiae or S. pombe ortholog identified). [GO:0071986]

Synonyms: Rag GEF; MAPKSP1/ROBLD3/C11orf59 complex; EGO complex; EGO-GSE complex; GSE complex; GTPase-containing complex for Gap1p sorting in the endosome

UniProt location names: UniProt Consortium, CC BY 4.0. Reference label and route sources.

Components

Component	Type	Part of / acts on	Grounding	Genes	Essential	Role
Ego1 Ragulator/EGO membrane-anchor subunit	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	EGO1	UNKNOWN	Yeast Ragulator/EGO constituent that anchors the Ego1/Ego2/Ego3 module associated with the Gtr1-Gtr2 Rag GTPase complex. `GO:0071986` Gene Ontology cellular-component term for the Ragulator complex, covering the yeast EGO/EGO-GSE complex that acts as a vacuolar Rag GTPase GEF. `DOI:10.1091/mbc.E15-07-0470` Kira et al. 2016 showed that Ego consists of Ego1, Ego3 and the newly identified Ego2 subunit, and followed Ego1/2/3 colocalization with Gtr1/2 and TORC1 on yeast vacuoles and associated puncta.
Ego2 Ragulator/EGO subunit	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	EGO2	UNKNOWN	Yeast Ragulator/EGO constituent required for full Gtr1-dependent TORC1 activation and vacuolar Gtr1 localization. `DOI:10.1091/mbc.E15-07-0470` Kira et al. 2016 showed that Ego consists of Ego1, Ego3 and the newly identified Ego2 subunit, and followed Ego1/2/3 colocalization with Gtr1/2 and TORC1 on yeast vacuoles and associated puncta.
Ego3 Ragulator/EGO subunit	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	EGO3	UNKNOWN	Yeast Ragulator/EGO constituent of the Ego1/Ego2/Ego3 vacuolar anchor module for Rag-family GTPase signaling. `GO:0071986` Gene Ontology cellular-component term for the Ragulator complex, covering the yeast EGO/EGO-GSE complex that acts as a vacuolar Rag GTPase GEF. `DOI:10.1091/mbc.E15-07-0470` Kira et al. 2016 showed that Ego consists of Ego1, Ego3 and the newly identified Ego2 subunit, and followed Ego1/2/3 colocalization with Gtr1/2 and TORC1 on yeast vacuoles and associated puncta.

Taxonomic distribution

NCBITaxon:4932 Saccharomyces cerevisiae — VARIABLE: Budding yeast is the curated model for the Ego1/Ego2/Ego3 Ragulator/EGO complex that anchors Rag-family GTPase signaling on the vacuole. [DOI:10.1091/mbc.E15-07-0470]

Canonical examples

NCBITaxon:4932 Saccharomyces cerevisiae — Yeast model in which Ego1, Ego2 and Ego3 colocalize with Gtr1/Gtr2 and TORC1 on the vacuole and associated puncta. [DOI:10.1091/mbc.E15-07-0470]

Functions

TORC1 signaling GO:0038202 — The yeast Ragulator/EGO complex anchors Rag-family GTPases at the vacuole during amino-acid-responsive TORC1 signaling.
- GO:0038202 Gene Ontology biological-process term for TORC1 signaling.
- GO:0071986 Gene Ontology cellular-component term for the Ragulator complex, covering the yeast EGO/EGO-GSE complex that acts as a vacuolar Rag GTPase GEF.
- DOI:10.1091/mbc.E15-07-0470 Kira et al. 2016 showed that Ego consists of Ego1, Ego3 and the newly identified Ego2 subunit, and followed Ego1/2/3 colocalization with Gtr1/2 and TORC1 on yeast vacuoles and associated puncta.

Mechanism graphs

Ragulator/EGO anchors yeast Gtr1-Gtr2 signaling (REGULATION)

In Saccharomyces cerevisiae, Ego1, Ego2 and Ego3 form the Ragulator/EGO anchor complex that localizes with Gtr1/Gtr2 and TORC1 on the vacuole during TORC1 regulation.

Subject	Predicate	Object	Evidence
Ego1 Ragulator/EGO membrane-anchor subunit	is part of	Ragulator complex	`GO:0071986` Gene Ontology cellular-component term for the Ragulator complex, covering the yeast EGO/EGO-GSE complex that acts as a vacuolar Rag GTPase GEF. `DOI:10.1091/mbc.E15-07-0470` Kira et al. 2016 showed that Ego consists of Ego1, Ego3 and the newly identified Ego2 subunit, and followed Ego1/2/3 colocalization with Gtr1/2 and TORC1 on yeast vacuoles and associated puncta.
Ego2 Ragulator/EGO subunit	is part of	Ragulator complex	`DOI:10.1091/mbc.E15-07-0470` Kira et al. 2016 showed that Ego consists of Ego1, Ego3 and the newly identified Ego2 subunit, and followed Ego1/2/3 colocalization with Gtr1/2 and TORC1 on yeast vacuoles and associated puncta.
Ego3 Ragulator/EGO subunit	is part of	Ragulator complex	`GO:0071986` Gene Ontology cellular-component term for the Ragulator complex, covering the yeast EGO/EGO-GSE complex that acts as a vacuolar Rag GTPase GEF. `DOI:10.1091/mbc.E15-07-0470` Kira et al. 2016 showed that Ego consists of Ego1, Ego3 and the newly identified Ego2 subunit, and followed Ego1/2/3 colocalization with Gtr1/2 and TORC1 on yeast vacuoles and associated puncta.
Ragulator complex	localizes to	fungal-type vacuole membrane	`GO:0071986` Gene Ontology cellular-component term for the Ragulator complex, covering the yeast EGO/EGO-GSE complex that acts as a vacuolar Rag GTPase GEF. `DOI:10.1091/mbc.E15-07-0470` Kira et al. 2016 showed that Ego consists of Ego1, Ego3 and the newly identified Ego2 subunit, and followed Ego1/2/3 colocalization with Gtr1/2 and TORC1 on yeast vacuoles and associated puncta.
Ragulator complex	regulates	Gtr1-Gtr2 GTPase complex	`GO:0071986` Gene Ontology cellular-component term for the Ragulator complex, covering the yeast EGO/EGO-GSE complex that acts as a vacuolar Rag GTPase GEF.
Ragulator complex	regulates	TORC1 signaling	`GO:0038202` Gene Ontology biological-process term for TORC1 signaling. `GO:0071986` Gene Ontology cellular-component term for the Ragulator complex, covering the yeast EGO/EGO-GSE complex that acts as a vacuolar Rag GTPase GEF. `DOI:10.1091/mbc.E15-07-0470` Kira et al. 2016 showed that Ego consists of Ego1, Ego3 and the newly identified Ego2 subunit, and followed Ego1/2/3 colocalization with Gtr1/2 and TORC1 on yeast vacuoles and associated puncta.

Discussions

CURATION_TODO (OPEN): Resolve exact source-neutral InterPro, Pfam, or NCBIfam groundings for the three yeast Ragulator/EGO subunit classes. This first Ragulator/EGO record names the yeast Ego1, Ego2 and Ego3 constituent subunits but leaves family grounding unset until exact orthology and paralogy boundaries are reviewed per subunit.
CURATION_TODO (RESOLVED): Keep the Gtr1-Gtr2 Rag-family GTPase heterodimer outside this Ragulator/EGO anchor record. GO:1990131 denotes the Gtr1-Gtr2 heterodimer, while GO:0071986 denotes the vacuolar Ragulator/EGO GEF complex; the GTPase heterodimer is a regulated neighbor rather than an anchor constituent.

Evidence

GO:0071986 Gene Ontology cellular-component term for the Ragulator complex, covering the yeast EGO/EGO-GSE complex that acts as a vacuolar Rag GTPase GEF.
GO:0032991 Gene Ontology cellular-component parent term for protein-containing complexes.
GO:0038202 Gene Ontology biological-process term for TORC1 signaling.
DOI:10.1091/mbc.E15-07-0470 Kira et al. 2016 showed that Ego consists of Ego1, Ego3 and the newly identified Ego2 subunit, and followed Ego1/2/3 colocalization with Gtr1/2 and TORC1 on yeast vacuoles and associated puncta.
DOI:10.1038/s41594-026-01746-2 Tafur et al. 2026 determined the yeast SEAC-EGOC supercomplex and separated the Gtr1/Gtr2 heterodimer from the Ego1/Ego2/Ego3 anchor within EGOC.

Curation history

2026-09-18T12:38:27Z codex · CREATED_RECORD — Scaffolded by scripts/new_record.py
2026-09-18T12:39:47Z codex · EXPANDED_RECORD — Added Ragulator/EGO synonymy, protein-complex parentage, reviewed-label-only yeast Ego1/Ego2/Ego3 components, Saccharomyces scope, a TORC1 signaling function, GO/DOI evidence, a nonmechanistic Gtr1-Gtr2/TORC1 regulation graph, and subunit-grounding plus Gtr1/Gtr2 boundary discussions.
2026-09-18T12:40:22Z codex · REFORMATTED_RECORD — Expanded shared YAML evidence aliases into plain repeated mappings.

Source: data/structures/other/ragulator_complex.yaml