RAVE complex

GO:0043291 · Other · Multiprotein complex · Proposed

A multisubunit complex that in Saccharomyces is composed of three subunits, Rav1p, Rav2p and Skp1p. Acts transiently to catalyze assembly of cytoplasmic V1 with membrane embedded V0 to form the V-ATPase holoenzyme. [GO:0043291]

Synonyms: regulator of the (H+)-ATPase of the vacuolar and endosomal membranes

Part of: GO:0005737 — cytoplasm (no local record)

UniProt location names: UniProt Consortium, CC BY 4.0. Reference label and route sources.

Components

Component	Type	Part of / acts on	Grounding	Genes	Essential	Role
Rav1 subunit	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	RAV1	UNKNOWN	Named constituent of the budding-yeast RAVE complex. `GO:0043291` GO:0043291 names Rav1p as a Saccharomyces RAVE subunit. `DOI:10.1038/35070067` Seol et al. 2001 isolated RAVE and identified Rav1p in the complex.
Rav2 subunit	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	RAV2	UNKNOWN	Named constituent of the budding-yeast RAVE complex. `GO:0043291` GO:0043291 names Rav2p as a Saccharomyces RAVE subunit. `DOI:10.1038/35070067` Seol et al. 2001 isolated RAVE and identified Rav2p in the complex.
Skp1 subunit	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	SKP1	UNKNOWN	Named Skp1-family constituent of the budding-yeast RAVE complex. `GO:0043291` GO:0043291 names Skp1p as a Saccharomyces RAVE subunit. `DOI:10.1038/35070067` Seol et al. 2001 isolated RAVE and identified Skp1p in the complex.

Taxonomic distribution

NCBITaxon:4932 Saccharomyces cerevisiae — COMMON: Budding yeast uses a cytoplasmic RAVE complex containing Rav1p, Rav2p and Skp1p to support V-ATPase V1-V0 assembly. [DOI:10.1038/35070067]

Canonical examples

NCBITaxon:4932 Saccharomyces cerevisiae — Yeast model in which RAVE was isolated as a Skp1-associated complex and connected to glucose-dependent V-ATPase assembly. [DOI:10.1038/35070067]

Functions

proton-transporting V-type ATPase complex assembly GO:0070070 — RAVE helps assemble cytosolic V1 sectors with membrane V0 sectors to form V-ATPase holoenzymes.
- GO:0043291 GO:0043291 defines RAVE by its transient catalysis of V1-V0 assembly into the V-ATPase holoenzyme.
- GO:0070070 GO:0070070 denotes proton-transporting V-type ATPase complex assembly.
- DOI:10.1074/jbc.M200682200 Smardon et al. 2002 provided biochemical and genetic evidence that RAVE is needed for stable assembly of the yeast V-ATPase.

Mechanism graphs

RAVE supports V-ATPase V1-V0 assembly (ASSEMBLY)

RAVE is a cytoplasmic yeast complex that helps assemble cytosolic V1 sectors with membrane V0 sectors into V-ATPase holoenzymes.

Subject	Predicate	Object	Evidence
Rav1 subunit	assembles into	RAVE complex	`GO:0043291` GO:0043291 names Rav1p as a Saccharomyces RAVE subunit.
Rav2 subunit	assembles into	RAVE complex	`GO:0043291` GO:0043291 names Rav2p as a Saccharomyces RAVE subunit.
Skp1 subunit	assembles into	RAVE complex	`GO:0043291` GO:0043291 names Skp1p as a Saccharomyces RAVE subunit.
RAVE complex	is part of	cytoplasm	`GO:0043291` GO:0043291 places RAVE part_of GO:0005737 cytoplasm.
RAVE complex	acts on	proton-transporting V-type ATPase, V1 domain	`DOI:10.1074/jbc.M200682200` Smardon et al. 2002 found reversible association of RAVE with cytosolic V1 complexes released by glucose deprivation.
proton-transporting V-type ATPase, V1 domain	assembles with	proton-transporting V-type ATPase, V0 domain	`GO:0043291` GO:0043291 defines RAVE by its role in assembly of cytoplasmic V1 with membrane embedded V0.
proton-transporting V-type ATPase complex assembly	produces	proton-transporting V-type ATPase complex	`GO:0043291` GO:0043291 defines RAVE by assembly of V1 and V0 into V-ATPase holoenzyme. `GO:0070070` GO:0070070 denotes proton-transporting V-type ATPase complex assembly.
RAVE complex	supports	proton-transporting V-type ATPase complex assembly	`GO:0043291` GO:0043291 defines RAVE by V-ATPase holoenzyme assembly. `DOI:10.1074/jbc.M200682200` Smardon et al. 2002 connected RAVE subunits to structural and functional V-ATPase assembly defects.

Discussions

CURATION_TODO (OPEN): Resolve exact source-neutral RAVE subunit family groundings for the reviewed label-only Rav1, Rav2 and Skp1 component rows. GO exactly grounds the RAVE complex and names the Saccharomyces cerevisiae Rav1p, Rav2p and Skp1p subunits; this first RAVE record adds reviewed label-only component rows but leaves exact taxon-agnostic family identifiers unset until they are verified.

Evidence

GO:0043291 Gene Ontology cellular-component term for the RAVE complex.
GO:0032991 Gene Ontology cellular-component parent term for protein-containing complexes.
GO:0005737 — cytoplasm Gene Ontology cellular-component term for the cytoplasm that contains RAVE.
GO:0070070 Gene Ontology biological-process term for proton-transporting V-type ATPase complex assembly.
GO:0033176 Gene Ontology cellular-component term for the proton-transporting V-type ATPase complex.
GO:0033180 Gene Ontology cellular-component term for the cytosolic V1 domain of a proton-transporting V-type ATPase.
GO:0033179 Gene Ontology cellular-component term for the membrane V0 domain of a proton-transporting V-type ATPase.
DOI:10.1038/35070067 Seol et al. 2001, 'Skp1 forms multiple protein complexes, including RAVE, a regulator of V-ATPase assembly', Nature Cell Biology.
DOI:10.1074/jbc.M200682200 Smardon, Tarsio and Kane 2002, 'The RAVE Complex Is Essential for Stable Assembly of the Yeast V-ATPase', Journal of Biological Chemistry.

Curation history

2026-09-18T20:00:12Z codex · CREATED_RECORD — Scaffolded by scripts/new_record.py
2026-09-18T20:00:57Z codex · EXPANDED_RECORD — Added RAVE synonymy, protein-complex parentage, cytoplasmic parthood, Saccharomyces scope, V-type ATPase assembly evidence, a nonmechanistic RAVE V1-V0 assembly graph, and a RAVE subunit-boundary TODO.
2026-09-18T20:12:38Z codex · ADDRESS_REVIEW — Resolved #1187 by adding reviewed label-only Rav1, Rav2 and Skp1 component rows. Resolved #1188 by connecting the RAVE graph to the assembled V-ATPase product node.

Source: data/structures/other/rave_complex.yaml