ribonuclease H2 complex

GO:0032299 · Other · Multiprotein complex · Proposed

A protein complex that possesses ribonuclease H activity, in which the catalytic subunit is a member of the RNase H2 (or HII) class. For example, in Saccharomyces the complex contains Rnh201p, Rnh202p and Rnh203p. [GO:0032299]

Synonyms: RNase H2 complex

UniProt location names: UniProt Consortium, CC BY 4.0. Reference label and route sources.

Functions

RNA-DNA hybrid ribonuclease activity GO:0004523 — The ribonuclease H2 complex catalyzes endonucleolytic cleavage of RNA in RNA-DNA hybrids.
- GO:0004523 GO:0004523 is the Gene Ontology molecular-function term for RNA-DNA hybrid ribonuclease activity.
- GO:0032299 GO:0032299 defines the ribonuclease H2 complex as a protein complex that possesses ribonuclease H activity.

Mechanism graphs

RNase H2 complex enables ribonuclease H activity (FUNCTION)

GO defines the ribonuclease H2 complex as a protein complex whose catalytic subunit is in the RNase H2/HII class and that possesses ribonuclease H activity.

Subject	Predicate	Object	Evidence
ribonuclease H2 complex	enables	RNA-DNA hybrid ribonuclease activity	`GO:0032299` GO:0032299 defines the complex by RNase H/HII catalytic-subunit activity. `GO:0004523` GO:0004523 is the molecular-function term for RNA-DNA hybrid ribonuclease activity.
RNA-DNA hybrid	is cleaved by	RNA-DNA hybrid ribonuclease activity	`GO:0004523` GO:0004523 defines the activity as endonucleolytic cleavage of RNA in RNA-DNA hybrids.

Discussions

CURATION_TODO (OPEN): Resolve exact source-neutral component boundaries for ribonuclease H2 complexes. The first GO:0032299 record captures the GO-defined RNase H2 complex identity and RNase H activity. Exact source-neutral families for the RNase H2/HII catalytic and accessory subunits should be curated from primary component evidence before assigning constituents.

Evidence

GO:0032299 Gene Ontology cellular-component term for the ribonuclease H2 complex and its exact RNase H2 complex synonym.
GO:0140535 Gene Ontology cellular-component term for intracellular protein-containing complexes.
GO:1902494 Gene Ontology cellular-component term for catalytic complexes.
GO:0004523 Gene Ontology molecular-function term for RNA-DNA hybrid ribonuclease activity.
PMID:14734815 Jeong et al. 2004, Ribonuclease H2 of Saccharomyces cerevisiae is a complex of three proteins, Nucleic Acids Research; PMID cited in the GO:0032299 definition xrefs.

Curation history

2026-09-25T15:18:50Z codex · CREATED_RECORD — Scaffolded by scripts/new_record.py
2026-09-25T15:19:51Z codex · EXPANDED_RECORD — Added GO exact synonymy, intracellular protein-containing and catalytic complex parentage, a GO-backed RNA-DNA hybrid ribonuclease function, GO/PubMed evidence, a nonmechanistic function graph and a component-boundary TODO.

Source: data/structures/other/ribonuclease_h2_complex.yaml