Swi5-Sfr1 complex

GO:0032798 · Other · Multiprotein complex · Proposed

A conserved DNA recombinase mediator complex that contains two Swi5 monomers and one Sfr1 monomer in Schizosaccharomyces, or orthologs thereof (e.g. Sae3p and Mei5p in Saccharomyces). [GO:0032798]

Synonyms: Sae3-Mei5 complex; Swi5 complex

UniProt location names: UniProt Consortium, CC BY 4.0. Reference label and route sources.

Components

Component	Type	Part of / acts on	Grounding	Genes	Stoichiometry	Essential	Role
Swi5 recombinase mediator subunit	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	swi5	2	UNKNOWN	Swi5-class constituent present as two monomers in the fission-yeast Swi5-Sfr1 recombinase mediator complex. `GO:0032798` GO:0032798 defines the Schizosaccharomyces complex as containing two Swi5 monomers. `DOI:10.1038/nsmb1136` Haruta et al. 2006 assayed the Schizosaccharomyces pombe Swi5-Sfr1 complex in Rhp51/Rad51- and Dmc1-mediated DNA strand exchange.
Sfr1 recombinase mediator subunit	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	sfr1	1	UNKNOWN	Sfr1-class constituent present as one monomer in the fission-yeast Swi5-Sfr1 recombinase mediator complex. `GO:0032798` GO:0032798 defines the Schizosaccharomyces complex as containing one Sfr1 monomer. `DOI:10.1038/nsmb1136` Haruta et al. 2006 assayed the Schizosaccharomyces pombe Swi5-Sfr1 complex in Rhp51/Rad51- and Dmc1-mediated DNA strand exchange.

Taxonomic distribution

NCBITaxon:4896 Schizosaccharomyces pombe — COMMON: Fission yeast has a two-subunit Swi5-Sfr1 complex that stimulates Rhp51/Rad51- and Dmc1-mediated DNA strand exchange in vitro. [DOI:10.1038/nsmb1136]

Canonical examples

NCBITaxon:4896 Schizosaccharomyces pombe — Reference fission-yeast system for in-vitro Swi5-Sfr1 DNA-strand-exchange mediator assays. [DOI:10.1038/nsmb1136]

Functions

DNA strand exchange activator activity GO:0140619 — The Swi5-Sfr1 complex stimulates Rhp51/Rad51- and Dmc1-mediated DNA strand exchange in vitro.
- GO:0140619 GO:0140619 is the Gene Ontology molecular-function term for DNA strand exchange activator activity.
- DOI:10.1038/nsmb1136 Haruta et al. 2006 reported that the fission-yeast Swi5-Sfr1 complex stimulates Rhp51/Rad51- and Dmc1-mediated DNA strand exchange in vitro.

Mechanism graphs

Swi5-Sfr1 stimulates recombinase strand exchange (FUNCTION)

The Schizosaccharomyces pombe Swi5 and Sfr1 proteins form a mediator complex that stimulates Rhp51/Rad51- and Dmc1-mediated DNA strand exchange.

Subject	Predicate	Object	Evidence
Swi5 recombinase mediator subunit	contributes to	Swi5-Sfr1 complex	`GO:0032798` GO:0032798 defines the Schizosaccharomyces complex as containing two Swi5 monomers.
Sfr1 recombinase mediator subunit	contributes to	Swi5-Sfr1 complex	`GO:0032798` GO:0032798 defines the Schizosaccharomyces complex as containing one Sfr1 monomer.
Swi5-Sfr1 complex	stimulates	DNA strand exchange activity	`DOI:10.1038/nsmb1136` Haruta et al. 2006 directly assayed Swi5-Sfr1 stimulation of Rhp51/Rad51- and Dmc1-mediated DNA strand exchange in vitro.
Swi5-Sfr1 complex	enables	DNA strand exchange activator activity	`GO:0140619` GO:0140619 is the molecular-function term for activity that increases DNA strand exchange activity. `DOI:10.1038/nsmb1136` Haruta et al. 2006 reported Swi5-Sfr1 stimulation of Rhp51/Rad51- and Dmc1-mediated DNA strand exchange.

Discussions

CURATION_TODO (OPEN): Resolve exact source-neutral family groundings for the Swi5 and Sfr1 constituents of the Swi5-Sfr1 complex. GO:0032798 exactly identifies the conserved Swi5-Sfr1 complex, but exact InterPro, Pfam, NCBIfam or UniProtKB family boundaries for the two Schizosaccharomyces-modeled subunit classes need narrower follow-up curation.

Evidence

GO:0032798 Gene Ontology cellular-component term for the Swi5-Sfr1 complex.
GO:0140513 Gene Ontology cellular-component parent term for nuclear protein-containing complexes.
GO:0140619 Gene Ontology molecular-function term for DNA strand exchange activator activity.
PMID:15620352 PubMed identifier for Hayase et al. 2004, the Cell paper on a Saccharomyces cerevisiae Mei5-Sae3 protein complex.
DOI:10.1016/j.cell.2004.10.031 DOI for Hayase et al. 2004, verified through PubMed.
PMID:16921379 PubMed identifier for Haruta et al. 2006, the Nature Structural & Molecular Biology paper on the Schizosaccharomyces pombe Swi5-Sfr1 complex.
DOI:10.1038/nsmb1136 DOI for Haruta et al. 2006, verified through PubMed.

Curation history

2026-09-27T12:09:09Z codex · CREATED_RECORD — Scaffolded by scripts/new_record.py
2026-09-27T12:10:33Z codex · EXPANDED_RECORD — Added GO Swi5-Sfr1 synonymy, nuclear protein-containing-complex parentage, two reviewed-label-only fission-yeast constituent rows, Schizosaccharomyces pombe scope, DNA-strand-exchange activator-function evidence, GO/PubMed/DOI evidence, a nonmechanistic function graph, and a Swi5-Sfr1 subunit-grounding TODO.

Source: data/structures/other/swi5_sfr1_complex.yaml