wc_medium

Overview

Applications

• Algae cultivation
• Phytoplankton culture
• Microalgae research

Synonyms

• {'name': 'dyiii_medium', 'source': 'unknown', 'original_category': 'algae'}
• {'name': 'modified_combo_medium', 'source': 'unknown', 'original_category': 'algae'}
• {'name': 'p49_medium', 'source': 'unknown', 'original_category': 'algae'}

Composition

flowchart LR
    CultureMech_000239["wc_medium"]
    ing0_1["1"]
    CultureMech_000239 --> ing0_1
    ing1_2["2"]
    CultureMech_000239 --> ing1_2
    ing2_3["3"]
    CultureMech_000239 --> ing2_3
    ing3_4["4"]
    CultureMech_000239 --> ing3_4
    ing4_5["5"]
    CultureMech_000239 --> ing4_5
    ing5_6["6"]
    CultureMech_000239 --> ing5_6
    ing6_7["7"]
    CultureMech_000239 --> ing6_7
    ing7_8["8"]
    CultureMech_000239 --> ing7_8
    ing8_9["9"]
    CultureMech_000239 --> ing8_9
    ing9_10["10"]
    CultureMech_000239 --> ing9_10
    ing10_11["11"]
    CultureMech_000239 --> ing10_11
    ing11_12["12"]
    CultureMech_000239 --> ing11_12

Ingredients (12)

Preparation

1. Important Notes To Consider: The quality of water, including natural seawater, is important.
2. The term 'dH2O' generally refers to distilled, deionized, distilled/deionized water, Milli-Q water (Millipore Corp.), etc.
3. Natural seawater and natural freshwater should be obtained from a non-polluted source. When dissolving chemicals, wait for the first component to dissolve before adding the second.
4. Stirring, and sometimes the addition of heat, is often required to dissolve the chemicals efficiently.
5. Preparation of stock solutions for frequently made algal culture media make preparing media convenient but also necessary to avoid errors from weighing very tiny amounts. Attention should be given to the pH of the final medium.
6. In most cases, if pH adjustment is required, this occurs before sterilization.
7. Please note that autoclaving removes carbon dioxide from media lacking carbonate stabilizing buffers.
8. This would make the medium very alkaline soon after removal from the autoclave.
9. In these cases, you should wait approximately 24 hours for gaseous equilibrium before inoculating the medium.

Target organisms

No target organisms are recorded.

Provenance

Merged from 4 source records.

• DYIII_Medium (CultureMech:000159)
• Modified_COMBO_Medium (CultureMech:000181)
• P49_Medium (CultureMech:000185)
• WC+_Medium (CultureMech:000239)