desulfovirga_medium

CultureMech:001289 bacterial DEFINED LIQUID
Original name: DESULFOVIRGA MEDIUM

Overview

External ontology
mediadive.medium:194a (source link unavailable) — DESULFOVIRGA MEDIUM
Applications
  • Microbial cultivation
Synonyms
  • {'name': 'desulfobulbus_medium', 'source': 'KOMODO', 'source_id': 'komodo.medium:194', 'original_category': 'bacterial'}
  • {'name': 'for_dsm_14880', 'source': 'KOMODO', 'source_id': 'komodo.medium:194.2', 'original_category': 'bacterial'}
  • {'name': 'for_dsm_1744', 'source': 'KOMODO', 'source_id': 'komodo.medium:194.1', 'original_category': 'bacterial'}
  • {'name': 'for_dsm_21556', 'source': 'KOMODO', 'source_id': 'komodo.medium:194.3', 'original_category': 'bacterial'}
  • {'name': 'medium_194_modified_for_dsm_13527', 'source': 'KOMODO', 'source_id': 'komodo.medium:194_13527', 'original_category': 'bacterial'}
  • {'name': 'medium_194_modified_for_dsm_5092', 'source': 'KOMODO', 'source_id': 'komodo.medium:194_5092', 'original_category': 'bacterial'}
  • {'name': 'medium_194_modified_for_dsm_6523', 'source': 'KOMODO', 'source_id': 'komodo.medium:194_6523', 'original_category': 'bacterial'}
pH range
{'min': 7.2, 'max': 7.4}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium194a.pdf

Composition

flowchart LR
    CultureMech_001289["desulfovirga_medium"]
    ing0_Na2SO4["Na2SO4"]
    CultureMech_001289 --> ing0_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing0_Na2SO4 -.-> CHEBI_32149
    ing1_KH2PO4["KH2PO4"]
    CultureMech_001289 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_NH4Cl["NH4Cl"]
    CultureMech_001289 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_NaCl["NaCl"]
    CultureMech_001289 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_001289 --> ing4_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing4_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing5_KCl["KCl"]
    CultureMech_001289 --> ing5_KCl
    CHEBI_32588["CHEBI:32588"]
    ing5_KCl -.-> CHEBI_32588
    ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001289 --> ing6_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing7_Sodium_resazurin["Sodium resazurin"]
    CultureMech_001289 --> ing7_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing7_Sodium_resazurin -.-> CHEBI_8806
    ing8_Na2CO3["Na2CO3"]
    CultureMech_001289 --> ing8_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing8_Na2CO3 -.-> CHEBI_29377
    ing9_m_Xylene["m-Xylene"]
    CultureMech_001289 --> ing9_m_Xylene
    CHEBI_28488["CHEBI:28488"]
    ing9_m_Xylene -.-> CHEBI_28488
    ing10_2_2_4_4_6_8_8_Heptamethylnonane["2,2,4,4,6,8,8-Heptamethylnonane"]
    CultureMech_001289 --> ing10_2_2_4_4_6_8_8_Heptamethylnonane
    CHEBI_131383["CHEBI:131383"]
    ing10_2_2_4_4_6_8_8_Heptamethylnonane -.-> CHEBI_131383
    ing11_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001289 --> ing11_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing11_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing12_H2SO4["H2SO4"]
    CultureMech_001289 --> ing12_H2SO4
    CHEBI_26836["CHEBI:26836"]
    ing12_H2SO4 -.-> CHEBI_26836
    ing13_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_001289 --> ing13_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing13_Na2S_x_9_H2O -.-> CHEBI_76209
    ing14_NaOH["NaOH"]
    CultureMech_001289 --> ing14_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing14_NaOH -.-> CHEBI_32145
    ing15_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_001289 --> ing15_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing15_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing16_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_001289 --> ing16_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing16_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing17_HCl["HCl"]
    CultureMech_001289 --> ing17_HCl
    CHEBI_17883["CHEBI:17883"]
    ing17_HCl -.-> CHEBI_17883
    ing18_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_001289 --> ing18_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing18_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing19_ZnCl2["ZnCl2"]
    CultureMech_001289 --> ing19_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing19_ZnCl2 -.-> CHEBI_49976
    ing20_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_001289 --> ing20_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing20_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing21_H3BO3["H3BO3"]
    CultureMech_001289 --> ing21_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing21_H3BO3 -.-> CHEBI_33118
    ing22_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_001289 --> ing22_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing22_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing23_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_001289 --> ing23_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing23_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing24_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001289 --> ing24_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing24_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing25_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001289 --> ing25_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing25_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing26_Vitamin_B12["Vitamin B12"]
    CultureMech_001289 --> ing26_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing26_Vitamin_B12 -.-> CHEBI_176843
    ing27_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_001289 --> ing27_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing27_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing28_D_____biotin["D-(+)-biotin"]
    CultureMech_001289 --> ing28_D_____biotin
    CHEBI_15956["CHEBI:15956"]
    ing28_D_____biotin -.-> CHEBI_15956
    ing29_Nicotinic_acid["Nicotinic acid"]
    CultureMech_001289 --> ing29_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing29_Nicotinic_acid -.-> CHEBI_15940
    CultureMech_001289 --> more["...3 more"]

Ingredients (33)

# Ingredient Concentration Ontology Notes
1 Na2SO4
2Na.O4S 142.043 g/mol
1.50215 G_PER_L CHEBI:32149
sodium sulfate
2 KH2PO4
H2O4P.K 136.084 g/mol
0.214592 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 NH4Cl
Cl.H4N 53.492 g/mol
0.321888 G_PER_L CHEBI:31206
ammonium chloride
4 NaCl
Cl.Na 58.443 g/mol
1.07296 G_PER_L CHEBI:26710
sodium chloride
5 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.429185 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
6 KCl
Cl.K 74.551 g/mol
0.536481 G_PER_L CHEBI:32588
potassium chloride
7 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.160944 G_PER_L CHEBI:86158
calcium chloride dihydrate
8 Sodium resazurin
C12H7NO4 229.191 g/mol
0.000536481 G_PER_L CHEBI:8806
Resazurin
9 Na2CO3
CO3.2Na 105.988 g/mol
50 G_PER_L CHEBI:29377
sodium carbonate
10 m-Xylene
C8H10 106.168 g/mol
0.3 G_PER_L CHEBI:28488
m-xylene
11 2,2,4,4,6,8,8-Heptamethylnonane
C16H34 226.448 g/mol
20 G_PER_L CHEBI:131383
2,2,4,4,6,8,8-heptamethylnonane
12 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
80 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
13 H2SO4
H2O4S 98.079 g/mol
10 G_PER_L CHEBI:26836
sulfuric acid
14 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
40 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
15 NaOH
HO.Na 39.997 g/mol
0.5 G_PER_L CHEBI:32145
sodium hydroxide
16 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.003 G_PER_L CHEBI:131361
disodium selenite pentahydrate
17 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.004 G_PER_L CHEBI:63939
sodium tungstate dihydrate
18 HCl
HCl 36.461 g/mol
2.5 G_PER_L CHEBI:17883
hydrogen chloride
19 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.5 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
20 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
21 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
22 H3BO3
H3BO3 61.833 g/mol
0.006 G_PER_L CHEBI:33118
boric acid
23 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
24 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
25 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
26 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate
27 Vitamin B12 0.1 G_PER_L CHEBI:176843
vitamin B12
28 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.08 G_PER_L CHEBI:30753
4-aminobenzoic acid
29 D-(+)-biotin
C10H16N2O3S 244.316 g/mol
0.02 G_PER_L CHEBI:15956
biotin
30 Nicotinic acid
C6H5NO2 123.111 g/mol
0.2 G_PER_L CHEBI:15940
nicotinic acid
31 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.1 G_PER_L CHEBI:31345
Calcium pantothenate
32 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.3 G_PER_L CHEBI:30961
pyridoxine hydrochloride
33 Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
0.2 G_PER_L CHEBI:132751
thiamine hydrochloride dihydrate

Preparation

  1. Solution A is sparged with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min ), then distributed under the same gas atmosphere in anoxic serum vials (e.g., 50 ml medium in 100 ml serum bottles) and autoclaved. Solutions B, D and G are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solutions E and F are prepared under 100% N2 gas atmosphere and sterilized by filtration. Solutions B to G are added to the sterile, cooled solution A in appropriate amounts in the sequence as indicated. Final pH of the medium should be 7.2 - 7.4.
  2. Note: For transfers use 5 - 10% (v/v) inoculum. Incubate tubes in a slanted position.
  3. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Target organisms

No target organisms are recorded.

Provenance

Merged from 8 source records.

TimestampCuratorActionNotes
2026-01-26T17:53:10.444052Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 194a
2026-03-13T06:19:02.875204Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001289
2026-03-17T06:30:57.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:14.735998+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "DESULFOVIRGA MEDIUM" to "desulfovirga_medium" for consistent matching
2026-05-20T06:01:15.045997+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '7.2-7.4' -> {'min': 7.2, 'max': 7.4}
2026-06-05T07:40:58.076520+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 32 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:56.140103+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:04:44.298925+00:00 recipe-merger Merged 8 duplicate recipes into canonical record Sources: KOMODO_194_DESULFOBULBUS_MEDIUM, desulfovirga_medium, for_dsm_14880, for_dsm_1744, for_dsm_21556, medium_194_modified_for_dsm_13527, medium_194_modified_for_dsm_5092, medium_194_modified_for_dsm_6523
2026-08-06T03:04:44.299049+00:00 merge_recipes.py MERGED_RECIPES merged 8 source recipe(s) on fingerprint 142b1f4d3f92f33f7fbbbe5d8a3f6cf9bc49d64adb02ab55c34dec6899098386