middlebrook_medium

Overview

External ontology

mediadive.medium:645 (source link unavailable) — MIDDLEBROOK MEDIUM

Applications

• Microbial cultivation

Synonyms

• {'name': 'hemin_medium_for_mycobacterium', 'source': 'KOMODO', 'source_id': 'komodo.medium:645a', 'original_category': 'bacterial'}
• {'name': 'medium_645_modified_for_dsm_44338', 'source': 'KOMODO', 'source_id': 'komodo.medium:645_44338', 'original_category': 'bacterial'}
• {'name': 'medium_645_modified_for_dsm_44339', 'source': 'KOMODO', 'source_id': 'komodo.medium:645_44339', 'original_category': 'bacterial'}
• {'name': 'medium_645_modified_for_dsm_44482', 'source': 'KOMODO', 'source_id': 'komodo.medium:645_44482', 'original_category': 'bacterial'}
• {'name': 'medium_645_modified_for_dsm_44483', 'source': 'KOMODO', 'source_id': 'komodo.medium:645_44483', 'original_category': 'bacterial'}
• {'name': 'medium_645_modified_for_dsm_44680', 'source': 'KOMODO', 'source_id': 'komodo.medium:645_44680', 'original_category': 'bacterial'}
• {'name': 'middelbrook_medium', 'source': 'KOMODO', 'source_id': 'komodo.medium:645', 'original_category': 'bacterial'}

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium645.pdf Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification.

Composition

flowchart LR
    CultureMech_001782["middlebrook_medium"]
    ing0_Bacto_Middlebrook_7H10_agar["Bacto Middlebrook 7H10 agar"]
    CultureMech_001782 --> ing0_Bacto_Middlebrook_7H10_agar
    CHEBI_2509["CHEBI:2509"]
    ing0_Bacto_Middlebrook_7H10_agar -.-> CHEBI_2509
    ing1_Glycerol["Glycerol"]
    CultureMech_001782 --> ing1_Glycerol
    CHEBI_17754["CHEBI:17754"]
    ing1_Glycerol -.-> CHEBI_17754
    ing2__NH4_2SO4["(NH4)2SO4"]
    CultureMech_001782 --> ing2__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing2__NH4_2SO4 -.-> CHEBI_62946
    ing3_KH2PO4["KH2PO4"]
    CultureMech_001782 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_Na2HPO4["Na2HPO4"]
    CultureMech_001782 --> ing4_Na2HPO4
    CHEBI_34683["CHEBI:34683"]
    ing4_Na2HPO4 -.-> CHEBI_34683
    ing5_Sodium_citrate["Sodium citrate"]
    CultureMech_001782 --> ing5_Sodium_citrate
    CHEBI_53258["CHEBI:53258"]
    ing5_Sodium_citrate -.-> CHEBI_53258
    ing6_MgSO4["MgSO4"]
    CultureMech_001782 --> ing6_MgSO4
    CHEBI_32599["CHEBI:32599"]
    ing6_MgSO4 -.-> CHEBI_32599
    ing7_CaCl2["CaCl2"]
    CultureMech_001782 --> ing7_CaCl2
    CHEBI_3312["CHEBI:3312"]
    ing7_CaCl2 -.-> CHEBI_3312
    ing8_ZnSO4["ZnSO4"]
    CultureMech_001782 --> ing8_ZnSO4
    CHEBI_35176["CHEBI:35176"]
    ing8_ZnSO4 -.-> CHEBI_35176
    ing9_CuSO4["CuSO4"]
    CultureMech_001782 --> ing9_CuSO4
    CHEBI_23414["CHEBI:23414"]
    ing9_CuSO4 -.-> CHEBI_23414
    ing10_L_Glutamic_acid["L-Glutamic acid"]
    CultureMech_001782 --> ing10_L_Glutamic_acid
    CHEBI_16015["CHEBI:16015"]
    ing10_L_Glutamic_acid -.-> CHEBI_16015
    ing11_Ferric_ammonium_citrate["Ferric ammonium citrate"]
    CultureMech_001782 --> ing11_Ferric_ammonium_citrate
    CHEBI_31604["CHEBI:31604"]
    ing11_Ferric_ammonium_citrate -.-> CHEBI_31604
    ing12_Pyridoxine["Pyridoxine"]
    CultureMech_001782 --> ing12_Pyridoxine
    CHEBI_16709["CHEBI:16709"]
    ing12_Pyridoxine -.-> CHEBI_16709
    ing13_Biotin["Biotin"]
    CultureMech_001782 --> ing13_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing13_Biotin -.-> CHEBI_15956
    ing14_Malachite_green["Malachite green"]
    CultureMech_001782 --> ing14_Malachite_green
    CHEBI_72449["CHEBI:72449"]
    ing14_Malachite_green -.-> CHEBI_72449
    ing15_Agar["Agar"]
    CultureMech_001782 --> ing15_Agar
    ing15_Agar -.-> CHEBI_2509
    ing16_Oleic_acid["Oleic acid"]
    CultureMech_001782 --> ing16_Oleic_acid
    CHEBI_16196["CHEBI:16196"]
    ing16_Oleic_acid -.-> CHEBI_16196
    ing17_Tryptone["Tryptone"]
    CultureMech_001782 --> ing17_Tryptone
    ing18_Yeast_extract["Yeast extract"]
    CultureMech_001782 --> ing18_Yeast_extract
    ing19_Sodium_chloride["Sodium chloride"]
    CultureMech_001782 --> ing19_Sodium_chloride
    CHEBI_26710["CHEBI:26710"]
    ing19_Sodium_chloride -.-> CHEBI_26710
    ing20_Dextrose["Dextrose"]
    CultureMech_001782 --> ing20_Dextrose
    CHEBI_17634["CHEBI:17634"]
    ing20_Dextrose -.-> CHEBI_17634
    ing21_Catalase["Catalase"]
    CultureMech_001782 --> ing21_Catalase
    ing22_NaCl["NaCl"]
    CultureMech_001782 --> ing22_NaCl
    ing22_NaCl -.-> CHEBI_26710

Ingredients (23)

Preparation

1. Sterilize for 15 min by 121°C cool to 50 - 55°C. Add 100 ml Middlebrook OADC enrichment (Becton Dickinson 212240) under aseptic conditions and mix thoroughly.
2. If you can´t order the commercial media then prepare the media in this way:
3. Final pH at 25°C 6.6 ± 0.2.

Target organisms

No target organisms are recorded.

Provenance

Merged from 8 source records.

• KOMODO_645a_HEMIN_medium_FOR_MYCOBACTERIUM (CultureMech:006191)
• medium_645_modified_for_dsm_44338 (CultureMech:006185)
• medium_645_modified_for_dsm_44339 (CultureMech:006186)
• medium_645_modified_for_dsm_44482 (CultureMech:006187)
• medium_645_modified_for_dsm_44483 (CultureMech:006188)
• medium_645_modified_for_dsm_44680 (CultureMech:006189)
• middelbrook_medium (CultureMech:006190)
• middlebrook_medium (CultureMech:001782)