acidiferrobacter_ms_medium

CultureMech:001811 bacterial DEFINED LIQUID
Original name: ACIDIFERROBACTER (MS) MEDIUM

Overview

External ontology
mediadive.medium:670 (source link unavailable) — ACIDIFERROBACTER (MS) MEDIUM
Applications
  • Microbial cultivation
Synonyms
  • {'name': 'ms_medium', 'source': 'KOMODO', 'source_id': 'komodo.medium:670', 'original_category': 'bacterial'}
  • {'name': 'for_dsm_2392_dsm_9464_and_dsm_9465', 'source': 'KOMODO', 'source_id': 'komodo.medium:670.2', 'original_category': 'bacterial'}
  • {'name': 'for_dsm_9468', 'source': 'KOMODO', 'source_id': 'komodo.medium:670.5', 'original_category': 'bacterial'}
pH
2.2
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium670.pdf

Composition

flowchart LR
    CultureMech_001811["acidiferrobacter_ms_medium"]
    ing0__NH4_2SO4["(NH4)2SO4"]
    CultureMech_001811 --> ing0__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing0__NH4_2SO4 -.-> CHEBI_62946
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001811 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2_K2HPO4["K2HPO4"]
    CultureMech_001811 --> ing2_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing2_K2HPO4 -.-> CHEBI_131527
    ing3_KCl["KCl"]
    CultureMech_001811 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001811 --> ing4_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing4_FeSO4_x_7_H2O -.-> CHEBI_75836

Ingredients (5)

# Ingredient Concentration Ontology Notes
1 (NH4)2SO4
2H4N.O4S 132.141 g/mol
2 G_PER_L CHEBI:62946
ammonium sulfate
2 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.25 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
3 K2HPO4
HO4P.2K 174.174 g/mol
0.1 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
4 KCl
Cl.K 74.551 g/mol
0.1 G_PER_L CHEBI:32588
potassium chloride
5 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
20 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate

Preparation

  1. Dissolve ingredients except ferrous sulfate and adjust pH to 2.2 with 4 N H2SO4. Sterilize medium by autoclaving for 15 min at 121°C. Before use, add FeSO4 x 7 H2O from a 40% (w/v) stock solution prepared with 0.2 N H2SO4 and filter-sterilized under 100% N2 gas atmosphere.
  2. Note: Incubate statically without shaking.

Target organisms

No target organisms are recorded.

Provenance

Merged from 4 source records.

TimestampCuratorActionNotes
2026-01-26T17:53:11.893371Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 670
2026-03-13T06:19:07.389283Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001811
2026-03-17T06:30:53.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:19.693487+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "ACIDIFERROBACTER (MS) MEDIUM" to "acidiferrobacter_ms_medium" for consistent matching
2026-06-05T07:40:47.192280+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:52.555678+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:04:57.178000+00:00 recipe-merger Merged 4 duplicate recipes into canonical record Sources: KOMODO_670_MS-MEDIUM, acidiferrobacter_ms_medium, for_dsm_2392_dsm_9464_and_dsm_9465, for_dsm_9468
2026-08-06T03:04:57.178048+00:00 merge_recipes.py MERGED_RECIPES merged 4 source recipe(s) on fingerprint 6505c14ef6ee4c34d1f3e91df87c6d87ec8b6b5291a77e19216d4cc89f4fcef1