Overview
- External ontology
- mediadive.medium:993 (source link unavailable) — ISP 5 MEDIUM
- Applications
-
- Microbial cultivation
- Synonyms
-
- {'name': 'acidic_glycerol_asparagine_agar_ph_5_0', 'source': 'MediaDive', 'source_id': 'mediadive.medium:J422', 'original_category': 'bacterial'}
- {'name': 'glycerol_asparagine_agar_isp_5', 'source': 'MediaDive', 'source_id': 'mediadive.medium:J60', 'original_category': 'bacterial'}
- pH range
- {'min': 7.0, 'max': 7.4}
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium993.pdf
Composition
flowchart LR
CultureMech_002175["isp_5_medium"]
ing0_L_Asparagine["L-Asparagine"]
CultureMech_002175 --> ing0_L_Asparagine
CHEBI_17196["CHEBI:17196"]
ing0_L_Asparagine -.-> CHEBI_17196
ing1_Glycerol["Glycerol"]
CultureMech_002175 --> ing1_Glycerol
CHEBI_17754["CHEBI:17754"]
ing1_Glycerol -.-> CHEBI_17754
ing2_K2HPO4["K2HPO4"]
CultureMech_002175 --> ing2_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing2_K2HPO4 -.-> CHEBI_131527
ing3_Agar["Agar"]
CultureMech_002175 --> ing3_Agar
CHEBI_2509["CHEBI:2509"]
ing3_Agar -.-> CHEBI_2509
ing4_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002175 --> ing4_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing4_FeSO4_x_7_H2O -.-> CHEBI_75836
ing5_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_002175 --> ing5_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing5_MnCl2_x_4_H2O -.-> CHEBI_86368
ing6_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_002175 --> ing6_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing6_ZnSO4_x_7_H2O -.-> CHEBI_32312
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
L-Asparagine
C4H8N2O3 132.119 g/mol
|
0.999001 G_PER_L |
CHEBI:17196
L-asparagine
|
Copied from referenced JCM Medium 60 |
| 2 |
Glycerol
C3H8O3 92.094 g/mol
|
9.99001 G_PER_L |
CHEBI:17754
glycerol
|
Copied from referenced JCM Medium 60 |
| 3 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.999001 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Copied from referenced JCM Medium 60 |
| 4 | Agar | 14.985 G_PER_L |
CHEBI:2509
agar
|
Copied from referenced JCM Medium 60 |
| 5 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Copied from referenced JCM Medium 60 |
| 6 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Copied from referenced JCM Medium 60 |
| 7 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Copied from referenced JCM Medium 60 |
Preparation
- The pH of trace salts solution is about 7.0-7.4. Do not adjust if it is within this range. Liquefy agar by steaming at 100°C for 15-20 minutes.
- Sterilize in flasks for pouring into Petri dishes. Seven standard 90 mm dishes containing 25 ml per dish.
Target organisms
No target organisms are recorded.
Provenance
Merged from 3 source records.
- acidic_glycerol_asparagine_agar_ph_5_0 (CultureMech:002775)
- glycerol_asparagine_agar_isp_5 (CultureMech:002957)
- isp_5_medium (CultureMech:002175)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:13.012522Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 993 |
| 2026-03-13T06:19:10.974837Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002175 |
| 2026-03-17T06:30:04.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:23.711712+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ISP 5 MEDIUM" to "isp_5_medium" for consistent matching |
| 2026-05-20T06:01:23.374367+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '7-7.4' -> {'min': 7.0, 'max': 7.4} |
| 2026-06-05T07:41:09.687617+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:00.151739+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:21.724535+00:00 | recipe-merger | Merged 3 duplicate recipes into canonical record | Sources: acidic_glycerol_asparagine_agar_ph_5_0, glycerol_asparagine_agar_isp_5, isp_5_medium |
| 2026-08-06T03:05:21.724581+00:00 | merge_recipes.py | MERGED_RECIPES | merged 3 source recipe(s) on fingerprint 7dc2930b3c4e05f17cb24459d1983f115e77e4b515d3421876d819174e2fa35e |