Overview
- External ontology
- komodo.medium:330 (source link unavailable) — RUMEN BACTERIA medium
- Applications
-
- Microbial cultivation
- Synonyms
-
- {'name': 'butyrivibrio_sp_medium', 'source': 'KOMODO', 'source_id': 'komodo.medium:704', 'original_category': 'bacterial'}
- {'name': 'butyrivibrio_sp_medium', 'source': 'MediaDive', 'source_id': 'mediadive.medium:704', 'original_category': 'bacterial'}
- {'name': 'medium_330_modified_for_dsm_17630', 'source': 'KOMODO', 'source_id': 'komodo.medium:330_17630', 'original_category': 'bacterial'}
- pH range
- {'min': 6.7, 'max': 6.8}
- Notes
- Source: KOMODO ModelSEED | ID: 330 | DSMZ Medium: 330 (mediadive.medium:330) | Aerobic: Yes Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_005024["rumen_bacteria_medium"]
ing0_K2HPO4["K2HPO4"]
CultureMech_005024 --> ing0_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing0_K2HPO4 -.-> CHEBI_131527
ing1_Trypticase_peptone["Trypticase peptone"]
CultureMech_005024 --> ing1_Trypticase_peptone
ing2_Yeast_extract["Yeast extract"]
CultureMech_005024 --> ing2_Yeast_extract
ing3_Glycerol["Glycerol"]
CultureMech_005024 --> ing3_Glycerol
CHEBI_17754["CHEBI:17754"]
ing3_Glycerol -.-> CHEBI_17754
ing4_Sodium_resazurin["Sodium resazurin"]
CultureMech_005024 --> ing4_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing4_Sodium_resazurin -.-> CHEBI_8806
ing5_Na2CO3["Na2CO3"]
CultureMech_005024 --> ing5_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing5_Na2CO3 -.-> CHEBI_29377
ing6_D_Glucose["D-Glucose"]
CultureMech_005024 --> ing6_D_Glucose
CHEBI_17634["CHEBI:17634"]
ing6_D_Glucose -.-> CHEBI_17634
ing7_Maltose["Maltose"]
CultureMech_005024 --> ing7_Maltose
CHEBI_17306["CHEBI:17306"]
ing7_Maltose -.-> CHEBI_17306
ing8_Cellobiose["Cellobiose"]
CultureMech_005024 --> ing8_Cellobiose
CHEBI_17057["CHEBI:17057"]
ing8_Cellobiose -.-> CHEBI_17057
ing9_Starch["Starch"]
CultureMech_005024 --> ing9_Starch
CHEBI_28017["CHEBI:28017"]
ing9_Starch -.-> CHEBI_28017
ing10_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_005024 --> ing10_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing10_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing11_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_005024 --> ing11_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing11_Na2S_x_9_H2O -.-> CHEBI_76209
ing12_KH2PO4["KH2PO4"]
CultureMech_005024 --> ing12_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing12_KH2PO4 -.-> CHEBI_63036
ing13_NaCl["NaCl"]
CultureMech_005024 --> ing13_NaCl
CHEBI_26710["CHEBI:26710"]
ing13_NaCl -.-> CHEBI_26710
ing14__NH4_2SO4["(NH4)2SO4"]
CultureMech_005024 --> ing14__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing14__NH4_2SO4 -.-> CHEBI_62946
ing15_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_005024 --> ing15_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing15_CaCl2_x_2_H2O -.-> CHEBI_86158
ing16_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_005024 --> ing16_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing16_MgSO4_x_7_H2O -.-> CHEBI_31795
ing17_Acetic_acid["Acetic acid"]
CultureMech_005024 --> ing17_Acetic_acid
CHEBI_15366["CHEBI:15366"]
ing17_Acetic_acid -.-> CHEBI_15366
ing18_Propionic_acid["Propionic acid"]
CultureMech_005024 --> ing18_Propionic_acid
CHEBI_30768["CHEBI:30768"]
ing18_Propionic_acid -.-> CHEBI_30768
ing19_Butyric_acid["Butyric acid"]
CultureMech_005024 --> ing19_Butyric_acid
CHEBI_30772["CHEBI:30772"]
ing19_Butyric_acid -.-> CHEBI_30772
ing20_n_Valeric_acid["n-Valeric acid"]
CultureMech_005024 --> ing20_n_Valeric_acid
CHEBI_17418["CHEBI:17418"]
ing20_n_Valeric_acid -.-> CHEBI_17418
ing21_iso_Butyric_acid["iso-Butyric acid"]
CultureMech_005024 --> ing21_iso_Butyric_acid
CHEBI_16135["CHEBI:16135"]
ing21_iso_Butyric_acid -.-> CHEBI_16135
ing22_Tryptone["Tryptone"]
CultureMech_005024 --> ing22_Tryptone
ing23_Yeast_extract["Yeast extract"]
CultureMech_005024 --> ing23_Yeast_extract
ing24_Sodium_chloride["Sodium chloride"]
CultureMech_005024 --> ing24_Sodium_chloride
ing24_Sodium_chloride -.-> CHEBI_26710
ing25_iso_Valeric_acid["iso-Valeric acid"]
CultureMech_005024 --> ing25_iso_Valeric_acid
CHEBI_28484["CHEBI:28484"]
ing25_iso_Valeric_acid -.-> CHEBI_28484
ing26_Haemin["Haemin"]
CultureMech_005024 --> ing26_Haemin
CHEBI_50385["CHEBI:50385"]
ing26_Haemin -.-> CHEBI_50385
ing27_NaOH["NaOH"]
CultureMech_005024 --> ing27_NaOH
CHEBI_32145["CHEBI:32145"]
ing27_NaOH -.-> CHEBI_32145
Ingredients (28)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.3 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 2 | Trypticase peptone | 2 G_PER_L | ||
| 3 | Yeast extract | 0.5 G_PER_L | ||
| 4 |
Glycerol
C3H8O3 92.094 g/mol
|
0.5 G_PER_L |
CHEBI:17754
glycerol
|
|
| 5 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.0005 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 6 |
Na2CO3
CO3.2Na 105.988 g/mol
|
4 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 7 |
D-Glucose
C6H12O6 180.156 g/mol
|
0.5 G_PER_L |
CHEBI:17634
D-glucose
|
|
| 8 |
Maltose
C12H22O11 342.297 g/mol
|
0.5 G_PER_L |
CHEBI:17306
maltose
|
|
| 9 |
Cellobiose
C12H22O11 342.297 g/mol
|
0.5 G_PER_L |
CHEBI:17057
cellobiose
|
|
| 10 | Starch | 0.5 G_PER_L |
CHEBI:28017
starch
|
(soluble) |
| 11 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.25 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 12 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.25 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 13 |
KH2PO4
H2O4P.K 136.084 g/mol
|
6 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 14 |
NaCl
Cl.Na 58.443 g/mol
|
12 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 15 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
6 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 16 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1.6 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 17 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
2.5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 18 |
Acetic acid
C2H4O2 60.052 g/mol
|
573.182 G_PER_L |
CHEBI:15366
acetic acid
|
|
| 19 |
Propionic acid
C3H6O2 74.079 g/mol
|
192.145 G_PER_L |
CHEBI:30768
propionic acid
|
|
| 20 |
Butyric acid
C4H8O2 88.106 g/mol
|
129 G_PER_L |
CHEBI:30772
butyric acid
|
|
| 21 |
n-Valeric acid
C5H10O2 102.133 g/mol
|
30.2827 G_PER_L |
CHEBI:17418
valeric acid
|
|
| 22 |
iso-Butyric acid
C4H8O2 88.106 g/mol
|
30.6375 G_PER_L |
CHEBI:16135
isobutyric acid
|
|
| 23 |
Tryptone
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
MICRO:0000182 (source link unavailable)
tryptone
|
Pancreatic digest of casein providing amino acids, peptides, and nitrogen; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 24 |
Yeast extract
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
5.0 G_PER_L |
FOODON:03315426
yeast extract
|
Autodigest of Saccharomyces cerevisiae providing vitamins, amino acids, and trace elements; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 25 |
Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium (Miller formulation); Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 26 |
iso-Valeric acid
C5H10O2 102.133 g/mol
|
30.0247 G_PER_L |
CHEBI:28484
isovaleric acid
|
|
| 27 |
Haemin
C34H32ClFeN4O4 651.952 g/mol
|
0.5 G_PER_L |
CHEBI:50385
hemin
|
|
| 28 |
NaOH
HO.Na 39.997 g/mol
|
1 G_PER_L |
CHEBI:32145
sodium hydroxide
|
Target organisms
No target organisms are recorded.
Provenance
Merged from 5 source records.
- KOMODO_330_RUMEN_BACTERIA_medium (CultureMech:005024)
- KOMODO_704_BUTYRIVIBRIO_SP._medium (CultureMech:006335)
- butyrivibrio_sp_medium (CultureMech:001843)
- medium_330_modified_for_dsm_17630 (CultureMech:005023)
- rumen_bacteria_medium (CultureMech:001429)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-14T05:58:58.561437+00:00 | idnf-grounding-fix-v1.0 | Fixed ID_NOT_FOUND term groundings | Absent/obsolete CHEBI term ids fixed - 1 re-grounded to the correct CHEBI, 0 de-grounded (mixture or unverifiable). |
| 2026-01-27T01:15:02.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 330 |
| 2026-02-03T11:19:19.385193 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 330 |
| 2026-03-13T06:19:29.615105Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:005024 |
| 2026-03-17T06:30:56.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:45.211102+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "RUMEN BACTERIA medium" to "rumen_bacteria_medium" for consistent matching |
| 2026-05-20T06:00:51.226666+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '6.7-6.8' -> {'min': 6.7, 'max': 6.8} |
| 2026-06-05T07:40:22.496588+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 21 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:44.602510+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:47.507011+00:00 | recipe-merger | Merged 5 duplicate recipes into canonical record | Sources: KOMODO_330_RUMEN_BACTERIA_medium, KOMODO_704_BUTYRIVIBRIO_SP._medium, butyrivibrio_sp_medium, medium_330_modified_for_dsm_17630, rumen_bacteria_medium |
| 2026-08-06T03:04:47.507100+00:00 | merge_recipes.py | MERGED_RECIPES | merged 5 source recipe(s) on fingerprint b6ddc39f7e7330d2a4ca2ac5546a92b958c579e8b4e1fee8bfc2869fc04d95a5 |