Overview
- External ontology
- TOGO:M1151 — Halalkaliarchaeum Medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- JCM Medium 1082 defines Halalkaliarchaeum Medium as sterilized JCM Medium 1079 Basal mineral NaCl medium plus JCM Medium 1081 Soda based mineral medium, 2 M sodium pyruvate, Trace vitamins from JCM Medium 197, and 10% yeast extract solution.
Ingredients (0)
No ingredients recorded.
Preparation
- Prepare and sterilize the JCM Medium 1079 basal mineral NaCl medium and JCM Medium 1081 soda based mineral medium.
- Mix 937.5 ml basal mineral NaCl medium with 62.5 ml soda based mineral medium per liter.
- Aseptically add the sodium pyruvate, Trace vitamins, and yeast extract solutions after autoclaving or filter sterilization.
Solutions
Basal mineral NaCl medium
Dose: 937.5 ML_PER_L
JCM Medium 1082 lists 937.5 ml/L sterilized Basal mineral NaCl medium from JCM Medium 1079.
Soda based mineral medium
Dose: 62.5 ML_PER_L
JCM Medium 1082 lists 62.5 ml/L sterilized Soda based mineral medium from JCM Medium 1081.
10% Yeast extract solution
Dose: 0.2 ML_PER_L
JCM Medium 1082 adds 0.2 ml/L 10% yeast extract solution after the basal media are sterilized.
2 M Sodium pyruvate solution
Dose: 5.0 ML_PER_L
JCM Medium 1082 adds 5.0 ml/L 2 M sodium pyruvate solution after the basal media are sterilized.
Trace vitamins
Dose: 10.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Biotin | 2.0 MG_PER_L | JCM Medium 197 prints this component in the Trace vitamins subrecipe. |
| Folic acid | 2.0 MG_PER_L | JCM Medium 197 prints this component in the Trace vitamins subrecipe. |
| Pyridoxine HCl | 10.0 MG_PER_L | JCM Medium 197 prints this component in the Trace vitamins subrecipe. |
| Thiamine HCl | 5.0 MG_PER_L | JCM Medium 197 prints this component in the Trace vitamins subrecipe. |
| Riboflavin | 5.0 MG_PER_L | JCM Medium 197 prints this component in the Trace vitamins subrecipe. |
| Nicotinic acid | 5.0 MG_PER_L | JCM Medium 197 prints this component in the Trace vitamins subrecipe. |
| Calcium pantothenate | 5.0 MG_PER_L | JCM Medium 197 prints this component in the Trace vitamins subrecipe. |
| Vitamin B12 | 0.1 MG_PER_L | JCM Medium 197 prints this component in the Trace vitamins subrecipe. |
| p-Aminobenzoic acid | 5.0 MG_PER_L | JCM Medium 197 prints this component in the Trace vitamins subrecipe. |
| Lipoic acid | 5.0 MG_PER_L | JCM Medium 197 prints this component in the Trace vitamins subrecipe. |
JCM Medium 1082 adds 10.0 ml/L Trace vitamins from JCM Medium 197.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.018558Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1151 |
| 2026-03-09T08:04:54.878915+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 5 solution(s) from ingredients |
| 2026-03-13T06:19:50.530056Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007675 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T19:46:08.911260+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Halalkaliarchaeum Medium" to "halalkaliarchaeum_medium" for consistent matching |
| 2026-05-16T09:03:07.953179+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-09-07T00:00:00-07:00 | repair_cross_reference_score25.py | RESOLVED_SCORE25_CROSS_REFERENCE | JCM Medium 1082 defines Halalkaliarchaeum Medium as sterilized JCM Medium 1079 Basal mineral NaCl medium plus JCM Medium 1081 Soda based mineral medium, 2 M sodium pyruvate, Trace vitamins from JCM Medium 197, and 10% yeast extract solution. |