microaerophilic_thermus_medium

CultureMech:010357 bacterial COMPLEX LIQUID
Original name: Microaerophilic Thermus Medium

Overview

External ontology
TOGO:M935 — Microaerophilic Thermus Medium
Applications
  • Microbial cultivation
pH
8.0
Notes
JCM Medium 894 / Microaerophilic Thermus Medium uses JCM Medium 276, adjusts the final medium to pH 8.0, and cultivates under a 99:1 N2/O2 gas atmosphere. MediaDive J894 and TOGO M935 report the same JCM recipe.

Composition

flowchart LR
    CultureMech_010357["microaerophilic_thermus_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_010357 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
    CultureMech_010357 --> ing1_Yeast_extract__BD_Difco_
    ing2_Tryptone__BD_Difco_["Tryptone (BD-Difco)"]
    CultureMech_010357 --> ing2_Tryptone__BD_Difco_
    ing3_Nitrogen_gas["Nitrogen gas"]
    CultureMech_010357 --> ing3_Nitrogen_gas
    CHEBI_17997["CHEBI:17997"]
    ing3_Nitrogen_gas -.-> CHEBI_17997
    ing4_Oxygen_gas["Oxygen gas"]
    CultureMech_010357 --> ing4_Oxygen_gas
    CHEBI_15379["CHEBI:15379"]
    ing4_Oxygen_gas -.-> CHEBI_15379

Ingredients (5)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
900.0 ML_PER_L CHEBI:15377
water
 TOGO M935 / JCM Medium 894 lists 900.0 ml/L Distilled water.
2 Yeast extract (BD-Difco) 1.0 G_PER_L FOODON:03315426
yeast extract
 TOGO M935 / JCM Medium 894 lists 1.0 g/L Yeast extract (BD-Difco).
3 Tryptone (BD-Difco) 1.0 G_PER_L MICRO:0000182 (source link unavailable)
tryptone
 TOGO M935 / JCM Medium 894 lists 1.0 g/L Tryptone (BD-Difco).
4 Nitrogen gas
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 TOGO M935 / JCM Medium 894 uses Nitrogen gas in a 99:1 gas atmosphere.
5 Oxygen gas
O2 31.998 g/mol
variable VARIABLE CHEBI:15379
dioxygen
 TOGO M935 / JCM Medium 894 uses Oxygen gas in a 99:1 gas atmosphere.

Preparation

  1. Use the JCM Medium 276 Castenholz formulation with 900.0 ml/L distilled water, 1.0 g/L BD-Difco yeast extract, 1.0 g/L BD-Difco tryptone, and 100.0 ml/L Castenholz basal salt solution.
  2. Adjust pH to 8.0 with NaOH.

Solutions

Castenholz basal salt solution

Dose: 100.0 ML_PER_L

CultureMech:013022

Stock composition
IngredientConcentrationNotes
Distilled water 1000.0 ML_PER_L JCM Medium 273 lists 1000.0 ml/L Distilled water.
MgSO4 x 7 H2O 1.0 G_PER_L JCM Medium 273 lists 1.0 g/L MgSO4 x 7 H2O.
NaCl 0.08 G_PER_L JCM Medium 273 lists 0.08 g/L NaCl.
NaNO3 6.89 G_PER_L JCM Medium 273 lists 6.89 g/L NaNO3.
Nitrilotriacetic acid 1.0 G_PER_L JCM Medium 273 lists 1.0 g/L Nitrilotriacetic acid.
KNO3 1.03 G_PER_L JCM Medium 273 lists 1.03 g/L KNO3.
Na2HPO4 1.11 G_PER_L JCM Medium 273 lists 1.11 g/L Na2HPO4.
CaSO4 x 2 H2O 0.6 G_PER_L JCM Medium 273 lists 0.6 g/L CaSO4 x 2 H2O.
TOGO M935 / JCM Medium 894 adds 100.0 ml/L Castenholz basal salt solution defined by JCM Medium 273.

FeCl3 x 6 H2O solution (0.03%)

Dose: 10.0 ML_PER_L

Stock composition
IngredientConcentrationNotes
FeCl3 x 6 H2O 0.03 PERCENT_W_V JCM Medium 273 lists 0.03 % w/v FeCl3 x 6 H2O.
JCM Medium 273 adds 10.0 ml/L FeCl3 x 6 H2O solution at 0.03% w/v.

Nitsch's trace elements

Dose: 10.0 ML_PER_L

CultureMech:013023

Stock composition
IngredientConcentrationNotes
Distilled water 1000.0 ML_PER_L JCM Medium 273 lists 1000.0 ml/L Distilled water.
Na2MoO4 x 2 H2O 0.025 G_PER_L JCM Medium 273 lists 0.025 g/L Na2MoO4 x 2 H2O.
H3BO3 0.5 G_PER_L JCM Medium 273 lists 0.5 g/L H3BO3.
CoCl2 x 6 H2O 0.046 G_PER_L JCM Medium 273 lists 0.046 g/L CoCl2 x 6 H2O.
ZnSO4 x 7 H2O 0.5 G_PER_L JCM Medium 273 lists 0.5 g/L ZnSO4 x 7 H2O.
CuSO4 x 5 H2O 0.016 G_PER_L JCM Medium 273 lists 0.016 g/L CuSO4 x 5 H2O.
MnSO4 x n H2O 2.2 G_PER_L JCM Medium 273 lists 2.2 g/L MnSO4 x n H2O.
H2SO4 0.5 ML_PER_L JCM Medium 273 lists 0.5 ml/L H2SO4.
JCM Medium 273 adds 10.0 ml/L Nitsch's trace elements.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:55.990789Z togo-import Imported from TOGO Medium Source: JCM, ID: M935
2026-01-28T19:48:10.008481Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: NaOH; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: Oxygen gas
2026-03-09T08:04:16.290437+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T06:20:13.244379Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:010357
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T06:29:46.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:32.222682+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Microaerophilic Thermus Medium" to "microaerophilic_thermus_medium" for consistent matching
2026-05-16T09:03:22.147263+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:45.830977+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-11T00:00:00-07:00 repair_togo_m935_microaerophilic_thermus_score15.py RESOLVED_TOGO_M935_MICROAEROPHILIC_THERMUS Corrected water from 900 g/L to 900.0 ml/L, removed NaOH from the ingredient list because it is only the pH titrant, grounded yeast extract, tryptone, and the 99:1 N2/O2 gases, expanded the Castenholz basal salt stock from JCM Medium 273, and linked the MediaDive J894 source duplicate.