All families / PF04016
PF04016: DUF364
Putative heavy-metal chelation
- Curation status
- SEEDED
- Seed status
- UNKNOWN CANDIDATE
- Characterization
- UNSCORED
Grounding
PF04016: Putative heavy-metal chelation
IPR007161: integrated entry for Putative heavy-metal chelation
Source description
This domain of unknown function has a PLP-dependent transferase-like fold. Its genomic context suggests that it may have a role in anaerobic vitamin B12 biosynthesis. This domain is often found at the C-terminus of proteins containing DUF4213, PF13938 (Putative heavy-metal chelation). The structure of UnioProtKB:B8FUJ5, PDB:3l5o, suggests that the protein has an enolase N-terminal-like fold and this Rossmann-like C-terminal domain. Structural and bioinformatic analyses reveal partial similarities to Rossmann-like methyltransferases, with residues from the enolase-like fold combining to form a unique active site that is likely to be involved in the condensation or hydrolysis of molecules implicated in the synthesis of flavins, pterins or other siderophores. The protein may be playing a role in heavy-metal chelation [InterPro reference PUB00075371].
Evidence and references
- InterPro entry and its literature references. PUB identifiers are internal InterPro references, not PubMed identifiers. Unresolved citations link to the originating entry.
- Frozen description source: InterPro/Pfam API
Source descriptions and links are provenance, not independent functional validation.
Evidence counts: not scored.
Curated functional assertions
No curated functional assertions supplied.
Protein sequence examples
1 verified sequence example. FASTA
| UniProt | Protein / organism | Sequence | Provenance |
|---|---|---|---|
| Q57923 | Uncharacterized protein MJ0500 Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) | 259 aa; version 1 UniProt 2026_03 | UNIPROT FETCH |
Pfam membership is not functional evidence. Whole-protein sequences do not establish domain boundaries. A coverage gap here is not absence from UniProt.
Sequences and metadata: UniProt Consortium, CC BY 4.0.
Domain matches
No residue-level domain match evidence is available in this build. Structure and AlphaFold counters do not supply coordinates or establish function.
Snapshot counters
- proteins
- 1,766
- matches
- 1,766
- proteomes
- 1,491
- taxa
- 2,706
- structures
- 2
- alphafold models
- 1,247
- domain architectures
- 6
Classification basis
short name matches duf; description says unknown function; domain of unknown function
No scoring reasons supplied.
Related Mech records
| Family | Mech | Record | Protein | Link basis |
|---|---|---|---|---|
| DUF364 (PF04016) | ProteinTraitsMech | Domain of unknown function DUF4213 | Q57923 Uncharacterized protein MJ0500 | canonical example family classification |
| DUF364 (PF04016) | ProteinTraitsMech | Putative heavy-metal chelation domain | Q57923 Uncharacterized protein MJ0500 | canonical example family classification |
| DUF364 (PF04016) | ProteinTraitsMech | Putative heavy-metal chelation domain | trait identifier | |
| DUF364 (PF04016) | ProteinTraitsMech | Putative heavy-metal chelation | Q57923 Uncharacterized protein MJ0500 | canonical example family classification |
| DUF364 (PF04016) | ProteinTraitsMech | Putative heavy-metal chelation | trait identifier | |
| DUF364 (PF04016) | ProteinTraitsMech | Putative heavy-metal chelation | Q57923 Uncharacterized protein MJ0500 | canonical example family classification |
| DUF364 (PF04016) | ProteinTraitsMech | CATH homologous superfamily 3.40.50.11590 | Q57923 Uncharacterized protein MJ0500 | canonical example family classification |
Curation history and reviews
No retained curation events supplied.
No retained review supplied.
Record source
Status and evidence
- Seed status
- A name-based classification of frozen InterPro/Pfam metadata. UNKNOWN CANDIDATE means the metadata explicitly says the function is unknown. KNOWN HISTORICAL DUF means a DUF name matched without that wording; this heuristic is not proof of a known function and does not establish experimental characterization. EX DUF means Pfam renamed the family away from a DUF/UPF name, as recorded in Pfam's previous identifiers; a rename usually follows published work on some members but is naming history, not proof of function for every member. Some EX DUF families' current names or descriptions still say the function is unknown; their classification basis lists this, and scoring does not treat them as characterized. FALSE POSITIVE TEXT HIT means the naming rules did not match.
- Characterization
- A separate evidence-scoring result. UNSCORED means no score has been calculated; it is not evidence that the family lacks a known function. Missing scores are not negative evidence. PARTIALLY CHARACTERIZED has partial support; KNOWN HISTORICAL DUF can inherit the seed label or reflect a known-function signal. Neither label implies experimental validation.
- Evidence counts
- Source-specific signals, not unique proteins, publications, or confidence scores. Known counts Rhea reactions and experimentally supported GO molecular-function annotations. Partial counts functional/domain assignments. Context counts structural, environmental, orthology, interaction and neighborhood signals; context alone does not assign function. Repeated annotations may not be independent.
- Curation status
- Reported separately from seed classification and characterization. SNAPSHOT ONLY means no curated metadata was supplied for this build. SEEDED denotes a source-derived record, not an expert-reviewed functional claim.
- Missing counts
- Not available means the source did not supply that counter. A zero is a reported count. Not scored means evidence counts are unavailable.