DUFMech

All families / PF09324

PF09324: Sec7-like_HDS

Mon2/Sec7/BIG1-like, HDS

Curation status
SEEDED
Seed status
EX DUF
Characterization
UNSCORED

Grounding

PF09324: Mon2/Sec7/BIG1-like, HDS
IPR015403: integrated entry for Mon2/Sec7/BIG1-like, HDS

Source description

This entry represents a HDS (homology downstream of Sec7) domain found towards the C-terminal of guanine nucleotide exchange factors involved Golgi transport, such as budding yeast protein Sec7, protein Mon2 and BIG1-like proteins [InterPro reference PUB00101185,InterPro reference PUB00101184]. Sec7 is involved in the secretory pathway as a protein binding scaffold for the COPII-COPI protein switch for maturation of the VTC intermediate compartments for Golgi compartment biogenesis [InterPro reference PUB00076747]. Sec7 has four HDS1-4 domains, which act to integrate the signals from several small GTPases, including Arf1 itself, to switch Sec7 from a strongly autoinhibited to a strongly auto activated form [InterPro reference PUB00101184].

Evidence and references

Source descriptions and links are provenance, not independent functional validation.

Evidence counts: not scored.

Curated functional assertions

No curated functional assertions supplied.

Protein sequence examples

12 verified sequence examples. FASTA

UniProtProtein / organismSequenceProvenance
A2A5R2Brefeldin A-inhibited guanine nucleotide-exchange protein 2
Mus musculus		1792 aa; version 1
UniProt 2026_03		PTM REGISTRY MIRROR
2 sibling references
F4IXW2Brefeldin A-inhibited guanine nucleotide-exchange protein 5
Arabidopsis thaliana		1739 aa; version 2
UniProt 2026_03		PTM REGISTRY MIRROR
2 sibling references
G3X9K3Brefeldin A-inhibited guanine nucleotide-exchange protein 1
Mus musculus		1846 aa; version 1
UniProt 2026_03		UNIPROT FETCH
2 sibling references
P11075ADP-ribosylation factor guanine nucleotide-exchange factor SEC7
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)		2009 aa; version 2
UniProt 2026_03		UNIPROT FETCH
1 sibling references
Q3UGY8Brefeldin A-inhibited guanine nucleotide-exchange protein 3
Mus musculus		2170 aa; version 1
UniProt 2026_03		UNIPROT FETCH
1 sibling references
Q7Z3U7Protein MON2 homolog
Homo sapiens		1717 aa; version 3
UniProt 2026_03		UNIPROT FETCH
8 sibling references
Q80TL7Protein MON2 homolog
Mus musculus		1715 aa; version 2
UniProt 2026_03		UNIPROT FETCH
1 sibling references
Q9LPC5Brefeldin A-inhibited guanine nucleotide-exchange protein 3
Arabidopsis thaliana		1750 aa; version 1
UniProt 2026_03		UNIPROT FETCH
1 sibling references
Q9LZX8Brefeldin A-inhibited guanine nucleotide-exchange protein 2
Arabidopsis thaliana		1793 aa; version 1
UniProt 2026_03		UNIPROT FETCH
1 sibling references
Q9VLT1Protein MON2 homolog
Drosophila melanogaster		1684 aa; version 4
UniProt 2026_03		UNIPROT FETCH
5 sibling references

First 10 shown; the FASTA and family JSON contain all examples.

Pfam membership is not functional evidence. Whole-protein sequences do not establish domain boundaries. A coverage gap here is not absence from UniProt.

Sequences and metadata: UniProt Consortium, CC BY 4.0.

Domain matches

No residue-level domain match evidence is available in this build. Structure and AlphaFold counters do not supply coordinates or establish function.

Snapshot counters

proteins
12,400
matches
12,422
proteomes
3,349
taxa
8,869
structures
1
alphafold models
1,000
domain architectures
230

Classification basis

pfam previous unknown name

No scoring reasons supplied.

Related Mech records

This family joined the worklist after the frozen cross-Mech scan, so other Mechs were not searched for it. Missing links here are not evidence that other Mechs lack it; rows shown come from mentions of a former DUF/UPF name.

No cross-Mech examples are present in the supplied snapshot.

Curation history and reviews

No retained curation events supplied.

No retained review supplied.

Record source

data/families/PF09324.yaml
Family YAML in this build

Download this family (JSON)

Status and evidence

Seed status
A name-based classification of frozen InterPro/Pfam metadata. UNKNOWN CANDIDATE means the metadata explicitly says the function is unknown. KNOWN HISTORICAL DUF means a DUF name matched without that wording; this heuristic is not proof of a known function and does not establish experimental characterization. EX DUF means Pfam renamed the family away from a DUF/UPF name, as recorded in Pfam's previous identifiers; a rename usually follows published work on some members but is naming history, not proof of function for every member. Some EX DUF families' current names or descriptions still say the function is unknown; their classification basis lists this, and scoring does not treat them as characterized. FALSE POSITIVE TEXT HIT means the naming rules did not match.
Characterization
A separate evidence-scoring result. UNSCORED means no score has been calculated; it is not evidence that the family lacks a known function. Missing scores are not negative evidence. PARTIALLY CHARACTERIZED has partial support; KNOWN HISTORICAL DUF can inherit the seed label or reflect a known-function signal. Neither label implies experimental validation.
Evidence counts
Source-specific signals, not unique proteins, publications, or confidence scores. Known counts Rhea reactions and experimentally supported GO molecular-function annotations. Partial counts functional/domain assignments. Context counts structural, environmental, orthology, interaction and neighborhood signals; context alone does not assign function. Repeated annotations may not be independent.
Curation status
Reported separately from seed classification and characterization. SNAPSHOT ONLY means no curated metadata was supplied for this build. SEEDED denotes a source-derived record, not an expert-reviewed functional claim.
Missing counts
Not available means the source did not supply that counter. A zero is a reported count. Not scored means evidence counts are unavailable.