All families / PF16016
PF16016: VASt
VAD1 Analog of StAR-related lipid transfer domain
- Curation status
- SEEDED
- Seed status
- EX DUF
- Characterization
- UNSCORED
Grounding
PF16016: VAD1 Analog of StAR-related lipid transfer domain
IPR031968: integrated entry for VAD1 Analog of StAR-related lipid transfer domain
Source description
The VASt (VAD1 Analog of StAR-related lipid transfer) domain is conserved across eukaryotes and is structurally related to Bet v1-like domains, including START lipid-binding domains. The 190-amino acid VASt domain is predominantly associated with lipid binding domains such as GRAM PF02893 (Pfam entry), C2 and PH domains. The VASt domain is likely to have a function in binding large hydrophobic ligands and may be specific for sterol [InterPro reference PUB00084279,InterPro reference PUB00084280]. The predicted structure of the VASt domain is a two-layer sandwich alpha beta fold, also called 'helix grip fold', containing three alpha helices (alpha1 to 3), six beta-sheets (beta1 to 6) and two loops (omega1 and 2) numbered from N to C terminus [InterPro reference PUB00084279]. Some proteins known to contain a VASt domain are : Plant vascular associated death1 (VAD1), a regulator of programmed cell death (PCD) harboring a GRAM putative lipid-binding domain. Yeast SNF1 Interacting Protein 3 (SIP3), may be involved in sterol transfer between intracellular membranes. Yeast Suicide Protein 1 (YSP1), a mitochondrial protein specifically required for the mitochondrial thread-grain transition, de-energization, and the cell death. May be involved in sterol transfer between intracellular membranes. Yeast Suicide Protein 2 (YSP2), a mitochondrial membrane protein involved in mitochondrial fragmentation and may be involved in sterol transfer between intracellular membranes.It is also found in human GramD1a-c.
Evidence and references
- InterPro entry and its literature references. PUB identifiers are internal InterPro references, not PubMed identifiers. Unresolved citations link to the originating entry.
- Frozen description source: InterPro/Pfam API
Source descriptions and links are provenance, not independent functional validation.
Evidence counts: not scored.
Curated functional assertions
No curated functional assertions supplied.
Protein sequence examples
8 verified sequence examples. FASTA
| UniProt | Protein / organism | Sequence | Provenance |
|---|---|---|---|
| F4HVW5 | Protein VASCULAR ASSOCIATED DEATH 1, chloroplastic Arabidopsis thaliana | 598 aa; version 1 UniProt 2026_03 | UNIPROT FETCH |
| P43560 | Membrane-anchored lipid-binding protein LAM5 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) | 674 aa; version 2 UniProt 2026_03 | PTM REGISTRY MIRROR |
| Q06681 | Membrane-anchored lipid-binding protein YSP2 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) | 1438 aa; version 1 UniProt 2026_03 | UNIPROT FETCH |
| Q08001 | Membrane-anchored lipid-binding protein LAM6 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) | 693 aa; version 1 UniProt 2026_03 | UNIPROT FETCH |
| Q3KR37 | Protein Aster-B Homo sapiens | 738 aa; version 1 UniProt 2026_03 | UNIPROT FETCH |
| Q80TI0 | Protein Aster-B Mus musculus | 738 aa; version 2 UniProt 2026_03 | UNIPROT FETCH |
| Q8VEF1 | Protein Aster-A Mus musculus | 722 aa; version 2 UniProt 2026_03 | UNIPROT FETCH |
| Q96CP6 | Protein Aster-A Homo sapiens | 724 aa; version 2 UniProt 2026_03 | UNIPROT FETCH |
Pfam membership is not functional evidence. Whole-protein sequences do not establish domain boundaries. A coverage gap here is not absence from UniProt.
Sequences and metadata: UniProt Consortium, CC BY 4.0.
Domain matches
No residue-level domain match evidence is available in this build. Structure and AlphaFold counters do not supply coordinates or establish function.
Snapshot counters
- proteins
- 12,938
- matches
- 14,430
- proteomes
- 3,299
- taxa
- 8,680
- structures
- 12
- alphafold models
- 9,903
- domain architectures
- 194
Classification basis
pfam previous unknown name
No scoring reasons supplied.
Related Mech records
This family joined the worklist after the frozen cross-Mech scan, so other Mechs were not searched for it. Missing links here are not evidence that other Mechs lack it; rows shown come from mentions of a former DUF/UPF name.
No cross-Mech examples are present in the supplied snapshot.
Curation history and reviews
No retained curation events supplied.
No retained review supplied.
Record source
Status and evidence
- Seed status
- A name-based classification of frozen InterPro/Pfam metadata. UNKNOWN CANDIDATE means the metadata explicitly says the function is unknown. KNOWN HISTORICAL DUF means a DUF name matched without that wording; this heuristic is not proof of a known function and does not establish experimental characterization. EX DUF means Pfam renamed the family away from a DUF/UPF name, as recorded in Pfam's previous identifiers; a rename usually follows published work on some members but is naming history, not proof of function for every member. Some EX DUF families' current names or descriptions still say the function is unknown; their classification basis lists this, and scoring does not treat them as characterized. FALSE POSITIVE TEXT HIT means the naming rules did not match.
- Characterization
- A separate evidence-scoring result. UNSCORED means no score has been calculated; it is not evidence that the family lacks a known function. Missing scores are not negative evidence. PARTIALLY CHARACTERIZED has partial support; KNOWN HISTORICAL DUF can inherit the seed label or reflect a known-function signal. Neither label implies experimental validation.
- Evidence counts
- Source-specific signals, not unique proteins, publications, or confidence scores. Known counts Rhea reactions and experimentally supported GO molecular-function annotations. Partial counts functional/domain assignments. Context counts structural, environmental, orthology, interaction and neighborhood signals; context alone does not assign function. Repeated annotations may not be independent.
- Curation status
- Reported separately from seed classification and characterization. SNAPSHOT ONLY means no curated metadata was supplied for this build. SEEDED denotes a source-derived record, not an expert-reviewed functional claim.
- Missing counts
- Not available means the source did not supply that counter. A zero is a reported count. Not scored means evidence counts are unavailable.