AbiV system

Edge evidence

• abiV locus contributes to AbiV antiphage activity RO:0002326

  The abiV locus encodes the protein required for the cloned phage-resistance phenotype.

  • DOI:10.1128/AEM.00780-08 A subsequent frameshift mutation in the ORF completely eliminated the phage resistance phenotype Haaber et al. abolish the phenotype with a frameshift in the AbiV open reading frame.
  • https://raw.githubusercontent.com/mdmparis/defense-finder-models/afb0e5a8b466be53586b13266f5d38d98c3ac268/Liste_hmm_system.md | AbiV__AbiV | AbiV__AbiV | AbiV | Custom | 20 | The DefenseFinder HMM inventory records the custom AbiV profile in the AbiV model namespace.
  • https://raw.githubusercontent.com/mdmparis/defense-finder-models/afb0e5a8b466be53586b13266f5d38d98c3ac268/DefenseFinder_rules.tsv AbiV AbiV 1 1 AbiV__AbiV The DefenseFinder rules table models AbiV as a single-profile system requiring the AbiV profile.
• sav phage gene contributes to AbiV-SaV complex formation RO:0002326

  The early phage sav gene encodes SaV, the phage protein needed for AbiV sensitivity.

  • DOI:10.1128/AEM.02093-08 we concluded that orf26 of phage p2 is involved in sensitivity to AbiV, and the gene was renamed sav Haaber et al. identify phage p2 orf26 as the AbiV sensitivity determinant.
  • DOI:10.1128/AEM.02093-08 Analyses of the sav regions in other AbiV-insensitive phage mutants from both the 936 and c2 groups revealed amino acid changes in the central region of the SaV protein Haaber et al. show that AbiV escape in multiple 936- and c2-like phages maps to sav homologues.
• AbiV antiphage activity contributes to AbiV-SaV complex formation RO:0002326

  AbiV directly and specifically interacts with SaV to form a stable AbiV-SaV complex.

  • DOI:10.1128/AEM.00093-10 they strongly and specifically interact with each other to form a stable protein complex Haaber et al. use SEC-MALS/UV/RI and fluorescence quenching to show direct AbiV-SaV complex formation.
• AbiV-SaV complex formation contributes to inhibited host translational machinery RO:0002326

  The AbiV-SaV complex inhibits host translational machinery after early sav expression.

  • DOI:10.1128/AEM.00093-10 A small amount of SaV is produced early and rapidly interacts with the host AbiV protein to form an active complex that inhibits the translational machinery of the cell Haaber et al. conclude that early SaV interacts with AbiV to form the active translation-inhibiting complex.
• inhibited host translational machinery contributes to blocked phage protein synthesis RO:0002326

  AbiV-SaV-mediated translation inhibition blocks early and late phage protein production.

  • DOI:10.1128/AEM.00093-10 Western blotting showed that translation of both early and late phage proteins was severely inhibited in the presence of AbiV Haaber et al. show that AbiV blocks translation of phage protein products including SaV, ORF11, and ORF16.
• blocked phage protein synthesis contributes to restricted phage propagation RO:0002326

  Blocked phage translation prevents completion of the AbiV-sensitive phage lytic program.

  • DOI:10.1128/AEM.00093-10 AbiV is an abortive infection protein that inhibits the lytic cycle of several virulent phages infecting Lactococcus lactis, while a mutation in the phage gene sav confers insensitivity to AbiV. In this study, we have further characterized the effects of the bacterial AbiV and its interaction with the phage p2 protein SaV. First, we showed that during phage infection of lactococcal AbiV⁺ cells, AbiV rapidly inhibited protein synthesis Haaber et al. frame the AbiV protein-synthesis block as part of AbiV inhibition of the phage lytic cycle.
• AbiV antiphage activity contributes to stalled phage DNA maturation RO:0002326

  AbiV activity prevents cleavage of replicated 936-like phage DNA into mature resolved DNA as a downstream infection-cycle defect.

  • DOI:10.1128/AEM.00780-08 The results described above showed that AbiV prevented cleavage of the replicated phage DNA Haaber et al. connect AbiV to failed cleavage of replicated phage DNA.
• stalled phage DNA maturation contributes to restricted phage propagation RO:0002326

  Accumulation of nonmature concatemeric phage DNA limits completion of AbiV-sensitive phage infection.

  • DOI:10.1128/AEM.00780-08 indicated that phage DNA accumulated in its concatemeric (nonmature) form in the resistant L. lactis cells Haaber et al. observed uncleaved concatemeric phage DNA in AbiV-containing resistant cells.
• restricted phage propagation confers AbiV system METPO:2007700

  Restriction of 936-like or c2-like phage propagation realizes the AbiV abortive-infection defense trait.

  • DOI:10.1128/AEM.00780-08 This ORF provided resistance against virulent lactococcal phages belonging to the 936 and c2 species with an efficiency of plaquing of 10−4 Haaber et al. support AbiV-mediated resistance to multiple 936- and c2-group lactococcal phages.
  • https://raw.githubusercontent.com/mdmparis/defense-finder-models/afb0e5a8b466be53586b13266f5d38d98c3ac268/List_system_article.md AbiV | 10\.1128/AEM\.00780-08 | AbiV, a novel antiphage abortive infection mechanism on the chromosome of Lactococcus lactis subsp\. cremoris MG1363 DefenseFinder records AbiV as a named antiphage abortive-infection system from the Haaber et al. paper.
• AbiV system is a abortive infection system rdfs:subClassOf

  AbiV system possession is an abortive-infection-system trait.

  • DOI:10.1128/AEM.00780-08 the gene was designated abiV and the Abi mechanism was designated AbiV Haaber et al. name the abiV-encoded phage resistance determinant as an Abi mechanism.