Brc113 system

traitmech:000522 · CLASS · PROPOSED

A phage defense system in which an organism possesses a gcu113/Brc113 bacteriophage-resistance integron cassette that supports growth during bacteriophage challenge.

Trait evidence (6)

DOI:10.1126/science.ads0915
brc113 L 95.1 70.8

Manual transcription of the Supplementary Fig. S2 Brc113 low-MOI E. coli IJ1862 heatmap row; the visible cells show reduced inhibition, including 95.1% for MS2 and 70.8% for F13.
DOI:10.1126/science.ads0915
defense profiles of identified Bacteriophage Resistance integron Cassettes (BRiCs) against a panel of different phages

Kieffer et al. classify positive integron cassettes as BRiCs and compare their defense profiles across a phage panel.
DOI:10.1126/science.ads0915
brc113 brc217 brc76 brcWGS21 brc22 brc23

Kieffer et al. include brc113 among identified BRiCs with predicted attC secondary structures.
https://www.science.org/doi/suppl/10.1126/science.ads0915/suppl_file/science.ads0915_sm.pdf
C936 pMBA brc113 E. coli IJ1862

Kieffer et al.'s supplementary construct table lists pMBA brc113 in E. coli IJ1862 for high/low-MOI phage-panel testing.
https://www.science.org/doi/suppl/10.1126/science.ads0915/suppl_file/science.ads0915_sm.pdf
C942 pMBA brc113 K. pneumoniae KP5

Kieffer et al.'s supplementary construct table lists the pMBA brc113 plasmid introduced into Klebsiella pneumoniae KP5.
https://www.science.org/doi/suppl/10.1126/science.ads0915/suppl_file/science.ads0915_sm.pdf
C974 pBTZ PcW brc113 P. aeruginosa PAO1

Kieffer et al.'s supplementary construct table lists the pBTZ PcW brc113 plasmid introduced into Pseudomonas aeruginosa PAO1.

Brc113 BRiCs confer phage resistance

Conservative system-level sketch linking a gcu113/Brc113 bacteriophage-resistance integron cassette to phage resistance and Brc113 system possession.

NONMECHANISTIC · The graph captures Brc113 at the integron-cassette and phage-resistance-output level without asserting the native host range, exact sensitive-phage breadth outside the reported panels, direct molecular substrate, or a DefenseFinder article/HMM/rules profile absent from the pinned snapshot.

Edge evidence

Brc113 integron cassette contributes to Brc113 phage resistance RO:0002326
The gcu113/Brc113 integron cassette contributes to phage resistance during phage-panel assays.
- DOI:10.1126/science.ads0915 brc113 L 95.1 70.8 Manual transcription of the Supplementary Fig. S2 Brc113 low-MOI E. coli IJ1862 heatmap row; the visible cells show reduced inhibition, including 95.1% for MS2 and 70.8% for F13.
Brc113 phage resistance confers Brc113 system METPO:2007700
Brc113-linked phage resistance realizes the Brc113 system possession trait.
- DOI:10.1126/science.ads0915 brc113 L 95.1 70.8 Manual transcription of the Supplementary Fig. S2 Brc113 low-MOI E. coli IJ1862 heatmap row; the visible cells show reduced inhibition, including 95.1% for MS2 and 70.8% for F13.
- DOI:10.1126/science.ads0915 defense profiles of identified Bacteriophage Resistance integron Cassettes (BRiCs) against a panel of different phages Kieffer et al. classify positive integron cassettes as BRiCs and compare their defense profiles across a phage panel.
Brc113 system is a phage defense system rdfs:subClassOf
Brc113 system possession is a phage-defense-system trait.
- DOI:10.1126/science.ads0915 defense profiles of identified Bacteriophage Resistance integron Cassettes (BRiCs) against a panel of different phages Kieffer et al. classify positive integron cassettes as BRiCs and compare their defense profiles across a phage panel.
- DOI:10.1126/science.ads0915 brc113 brc217 brc76 brcWGS21 brc22 brc23 Kieffer et al. include brc113 among identified BRiCs with predicted attC secondary structures.

Provenance

Identifier source: TraitMech local identifier
Definition source: DOI:10.1126/science.ads0915

Parent traits (1)

phage defense system traitmech:000209

Synonyms (2)

gcu113 RELATED_SYNONYM · DOI:10.1126/science.ads0915
brc113 RELATED_SYNONYM · DOI:10.1126/science.ads0915

kg-microbe context

No kg-microbe node embedding matched this record in the 2026-04-25 deepwalk.

Discussions and Knowledge Gaps (1)

Open questions attached to this trait. Seeded by just knowledge-gap-scan and curated; see the corpus-wide index.

Resolve Brc113 native host breadth, exact sensitive-phage breadth, molecular output, and DefenseFinder article/HMM/rules coverage before minting narrower Brc113 mechanism children.

KNOWLEDGE GAP OPEN brc113-defensefinder-model-gap · raised by codex · 2026-10-01

Attached to causal_graphs#brc113_integron_cassette_confers_phage_resistance

Kieffer et al. identify brc113 as a bacteriophage-resistance integron cassette. The pinned DefenseFinder article registry, HMM inventory, and rules table have no exact gcu113 or Brc113 row, so the first-pass record leaves native-host breadth, profile boundaries, exact phage target breadth, and direct molecular output unresolved.

Evidence

DOI:10.1126/science.ads0915
brc113 L 95.1 70.8
DOI:10.1126/science.ads0915
defense profiles of identified Bacteriophage Resistance integron Cassettes (BRiCs) against a panel of different phages
DOI:10.1126/science.ads0915
brc113 brc217 brc76 brcWGS21 brc22 brc23
https://www.science.org/doi/suppl/10.1126/science.ads0915/suppl_file/science.ads0915_sm.pdf
C942 pMBA brc113 K. pneumoniae KP5
https://www.science.org/doi/suppl/10.1126/science.ads0915/suppl_file/science.ads0915_sm.pdf
C974 pBTZ PcW brc113 P. aeruginosa PAO1
https://raw.githubusercontent.com/mdmparis/defense-finder-models/afb0e5a8b466be53586b13266f5d38d98c3ac268/List_system_article.md
https://raw.githubusercontent.com/mdmparis/defense-finder-models/afb0e5a8b466be53586b13266f5d38d98c3ac268/Liste_hmm_system.md
https://raw.githubusercontent.com/mdmparis/defense-finder-models/afb0e5a8b466be53586b13266f5d38d98c3ac268/DefenseFinder_rules.tsv

Curation history

2026-10-01T18:33:44Z · MINTED_TRAITMECH_ID · codex
Minted Brc113 system as a DOI-backed GENOMICS TraitRecord under phage defense system after an ignored-and-hidden duplicate review found no exact live TraitMech, METPO, history, or prior proposal record; kept the graph at BRiC integron-cassette level because the pinned DefenseFinder article registry, HMM inventory, and rules table have no exact Brc113 row; the replacement placeholder is reserved in proposals/metpo_traitmech_v399.
2026-10-01T18:33:45Z · REVIEW_CANONICAL_EXAMPLE_EVIDENCE_GAP · codex
Reviewed Brc113 system during initial curation and left canonical_examples empty because the sources support heterologous gcu113/Brc113 phage-resistance assays but not a direct named native microbial isolate exemplar with experimentally verified endogenous Brc113 activity. No paid research was used.