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Brc217 system

traitmech:000521 · CLASS · PROPOSED

A phage defense system in which an organism possesses a gcu217/Brc217 bacteriophage-resistance integron cassette that supports growth during bacteriophage challenge.

Trait evidence (6)

  • DOI:10.1126/science.ads0915
    brc217 L 97.6 44.2 96.6 56.5

    Manual transcription of the Supplementary Fig. S2 Brc217 low-MOI E. coli IJ1862 heatmap row; the visible cells show reduced inhibition, including 97.6% for P1, 44.2% for phi80, 96.6% for HK544, and 56.5% for F13.

  • DOI:10.1126/science.ads0915
    brc217 H 91.2 96.7

    Manual transcription of the Supplementary Fig. S2 Brc217 high-MOI E. coli IJ1862 heatmap row; the visible cells show reduced inhibition, including 91.2% for HK544 and 96.7% for F13.

  • DOI:10.1126/science.ads0915
    defense profiles of identified Bacteriophage Resistance integron Cassettes (BRiCs) against a panel of different phages

    Kieffer et al. classify positive integron cassettes as BRiCs and compare their defense profiles across a phage panel.

  • DOI:10.1126/science.ads0915
    brc113 brc217 brc76 brcWGS21 brc22 brc23

    Kieffer et al. include brc217 among identified BRiCs with predicted attC secondary structures.

  • https://www.science.org/doi/suppl/10.1126/science.ads0915/suppl_file/science.ads0915_sm.pdf
    C934 pMBA brc217 E. coli IJ1862

    Kieffer et al.'s supplementary construct table lists pMBA brc217 in E. coli IJ1862 for high/low-MOI phage-panel testing.

  • DOI:10.1126/science.ads0915
    C940 pMBA brc217 K. pneumoniae KP5

    Kieffer et al.'s supplementary construct table lists the pMBA brc217 plasmid introduced into Klebsiella pneumoniae KP5.

Brc217 BRiCs confer phage resistance

Conservative system-level sketch linking a gcu217/Brc217 bacteriophage-resistance integron cassette to phage resistance and Brc217 system possession.

NONMECHANISTIC · The graph captures Brc217 at the integron-cassette and phage-resistance-output level without asserting the native host range, exact sensitive-phage breadth outside the reported panels, direct molecular substrate, or a DefenseFinder article/HMM/rules profile absent from the pinned snapshot.

Brc217 BRiCs confer phage resistance Interactive directed graph showing evidence-backed causal relationships for Brc217 system.

Edge evidence

  • Brc217 integron cassette contributes to Brc217 phage resistance RO:0002326

    The gcu217/Brc217 integron cassette contributes to phage resistance during phage-panel assays.

    • DOI:10.1126/science.ads0915 brc217 L 97.6 44.2 96.6 56.5 Manual transcription of the Supplementary Fig. S2 Brc217 low-MOI E. coli IJ1862 heatmap row; the visible cells show reduced inhibition, including 97.6% for P1, 44.2% for phi80, 96.6% for HK544, and 56.5% for F13.
    • DOI:10.1126/science.ads0915 brc217 H 91.2 96.7 Manual transcription of the Supplementary Fig. S2 Brc217 high-MOI E. coli IJ1862 heatmap row; the visible cells show reduced inhibition, including 91.2% for HK544 and 96.7% for F13.
  • Brc217 phage resistance confers Brc217 system METPO:2007700

    Brc217-linked phage resistance realizes the Brc217 system possession trait.

    • DOI:10.1126/science.ads0915 brc217 L 97.6 44.2 96.6 56.5 Manual transcription of the Supplementary Fig. S2 Brc217 low-MOI E. coli IJ1862 heatmap row; the visible cells show reduced inhibition, including 97.6% for P1, 44.2% for phi80, 96.6% for HK544, and 56.5% for F13.
    • DOI:10.1126/science.ads0915 brc217 H 91.2 96.7 Manual transcription of the Supplementary Fig. S2 Brc217 high-MOI E. coli IJ1862 heatmap row; the visible cells show reduced inhibition, including 91.2% for HK544 and 96.7% for F13.
    • DOI:10.1126/science.ads0915 defense profiles of identified Bacteriophage Resistance integron Cassettes (BRiCs) against a panel of different phages Kieffer et al. classify positive integron cassettes as BRiCs and compare their defense profiles across a phage panel.
  • Brc217 system is a phage defense system rdfs:subClassOf

    Brc217 system possession is a phage-defense-system trait.

    • DOI:10.1126/science.ads0915 defense profiles of identified Bacteriophage Resistance integron Cassettes (BRiCs) against a panel of different phages Kieffer et al. classify positive integron cassettes as BRiCs and compare their defense profiles across a phage panel.
    • DOI:10.1126/science.ads0915 brc113 brc217 brc76 brcWGS21 brc22 brc23 Kieffer et al. include brc217 among identified BRiCs with predicted attC secondary structures.

Provenance

Identifier source
TraitMech local identifier
Definition source
DOI:10.1126/science.ads0915

Parent traits (1)

Synonyms (2)

  • gcu217 RELATED_SYNONYM · DOI:10.1126/science.ads0915
  • brc217 RELATED_SYNONYM · DOI:10.1126/science.ads0915

kg-microbe context

No kg-microbe node embedding matched this record in the 2026-04-25 deepwalk.

Discussions and Knowledge Gaps (1)

Open questions attached to this trait. Seeded by just knowledge-gap-scan and curated; see the corpus-wide index.

Resolve Brc217 native host breadth, exact sensitive-phage breadth, molecular output, and DefenseFinder article/HMM/rules coverage before minting narrower Brc217 mechanism children.

KNOWLEDGE GAP OPEN brc217-defensefinder-model-gap · raised by codex · 2026-10-01

Attached to causal_graphs#brc217_integron_cassette_confers_phage_resistance

Kieffer et al. identify brc217 as a bacteriophage-resistance integron cassette. The pinned DefenseFinder article registry, HMM inventory, and rules table have no exact gcu217 or Brc217 row, so the first-pass record leaves native-host breadth, profile boundaries, exact phage target breadth, and direct molecular output unresolved.

Evidence

Curation history

  1. · MINTED_TRAITMECH_ID · codex

    Minted Brc217 system as a DOI-backed GENOMICS TraitRecord under phage defense system after an ignored-and-hidden duplicate review found no exact live TraitMech, METPO, history, or prior proposal record; kept the graph at BRiC integron-cassette level because the pinned DefenseFinder article registry, HMM inventory, and rules table have no exact Brc217 row; the replacement placeholder is reserved in proposals/metpo_traitmech_v398.

  2. · REVIEW_CANONICAL_EXAMPLE_EVIDENCE_GAP · codex

    Reviewed Brc217 system during initial curation and left canonical_examples empty because the sources support heterologous gcu217/Brc217 phage-resistance assays but not a direct named native microbial isolate exemplar with experimentally verified endogenous Brc217 activity. No paid research was used.