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Brc233 system

traitmech:000518 · CLASS · PROPOSED

A phage defense system in which an organism possesses a gcu233/Brc233 bacteriophage-resistance integron cassette that supports growth during bacteriophage challenge.

Trait evidence (6)

  • DOI:10.1126/science.ads0915
    brc233 L 27.7 51.8 37.3 62.6 95.7 12.8

    Manual transcription of the Supplementary Fig. S2 Brc233 low-MOI E. coli IJ1862 heatmap row; the row shows reduced inhibition, including 27.7% for MS2, 51.8% for G4, 37.3% for T4, 62.6% for F1, and 12.8% for F13.

  • DOI:10.1126/science.ads0915
    brc233 H 34.0 52.2 89.4 39.4 98.2 41.3

    Manual transcription of the Supplementary Fig. S2 Brc233 high-MOI E. coli IJ1862 heatmap row; the row shows reduced inhibition, including 34.0% for MS2, 52.2% for G4, 39.4% for F1, and 41.3% for F13.

  • DOI:10.1126/science.ads0915
    defense profiles of identified Bacteriophage Resistance integron Cassettes (BRiCs) against a panel of different phages

    Kieffer et al. classify positive integron cassettes as BRiCs and compare their defense profiles across a phage panel.

  • DOI:10.1126/science.ads0915
    C807 pMBA brc233 E. coli IJ1862

    Kieffer et al.'s supplementary construct table lists pMBA brc233 in E. coli IJ1862 for high/low-MOI phage-panel testing.

  • DOI:10.1126/science.ads0915
    C817 pMBA brc233 K. pneumoniae

    Kieffer et al.'s supplementary construct table lists the pMBA brc233 plasmid introduced into Klebsiella pneumoniae KP5.

  • https://raw.githubusercontent.com/mdmparis/defense-finder-models/afb0e5a8b466be53586b13266f5d38d98c3ac268/List_system_article.md
    | gcu233 | 10\.1126/science\.ads0915 | Mobile integrons encode phage defense systems |

    The pinned DefenseFinder article registry maps the gcu233 source key to the Kieffer et al. mobile-integron BRiC paper.

Brc233 BRiCs confer phage resistance

Conservative system-level sketch linking a gcu233/Brc233 bacteriophage-resistance integron cassette to phage resistance and Brc233 system possession.

NONMECHANISTIC · The graph captures Brc233 at the integron-cassette and phage-resistance-output level without asserting the native host range, exact sensitive-phage breadth outside the reported panels, direct molecular substrate, or a DefenseFinder HMM/rules profile absent from the pinned snapshot.

Brc233 BRiCs confer phage resistance Interactive directed graph showing evidence-backed causal relationships for Brc233 system.

Edge evidence

  • Brc233 integron cassette contributes to Brc233 phage resistance RO:0002326

    The gcu233/Brc233 integron cassette contributes to phage resistance during phage-panel assays.

    • DOI:10.1126/science.ads0915 brc233 L 27.7 51.8 37.3 62.6 95.7 12.8 Manual transcription of the Supplementary Fig. S2 Brc233 low-MOI E. coli IJ1862 heatmap row; the row shows reduced inhibition, including 27.7% for MS2, 51.8% for G4, 37.3% for T4, 62.6% for F1, and 12.8% for F13.
    • DOI:10.1126/science.ads0915 brc233 H 34.0 52.2 89.4 39.4 98.2 41.3 Manual transcription of the Supplementary Fig. S2 Brc233 high-MOI E. coli IJ1862 heatmap row; the row shows reduced inhibition, including 34.0% for MS2, 52.2% for G4, 39.4% for F1, and 41.3% for F13.
  • Brc233 phage resistance confers Brc233 system METPO:2007700

    Brc233-linked phage resistance realizes the Brc233 system possession trait.

    • DOI:10.1126/science.ads0915 brc233 L 27.7 51.8 37.3 62.6 95.7 12.8 Manual transcription of the Supplementary Fig. S2 Brc233 low-MOI E. coli IJ1862 heatmap row; the row shows reduced inhibition, including 27.7% for MS2, 51.8% for G4, 37.3% for T4, 62.6% for F1, and 12.8% for F13.
    • DOI:10.1126/science.ads0915 brc233 H 34.0 52.2 89.4 39.4 98.2 41.3 Manual transcription of the Supplementary Fig. S2 Brc233 high-MOI E. coli IJ1862 heatmap row; the row shows reduced inhibition, including 34.0% for MS2, 52.2% for G4, 39.4% for F1, and 41.3% for F13.
    • DOI:10.1126/science.ads0915 defense profiles of identified Bacteriophage Resistance integron Cassettes (BRiCs) against a panel of different phages Kieffer et al. classify positive integron cassettes as BRiCs and compare their defense profiles across a phage panel.
  • Brc233 system is a phage defense system rdfs:subClassOf

    Brc233 system possession is a phage-defense-system trait.

Provenance

Identifier source
TraitMech local identifier
Definition source
DOI:10.1126/science.ads0915

Parent traits (1)

Synonyms (2)

  • gcu233 RELATED_SYNONYM · https://raw.githubusercontent.com/mdmparis/defense-finder-models/afb0e5a8b466be53586b13266f5d38d98c3ac268/List_system_article.md
  • brc233 RELATED_SYNONYM · DOI:10.1126/science.ads0915

kg-microbe context

No kg-microbe node embedding matched this record in the 2026-04-25 deepwalk.

Discussions and Knowledge Gaps (1)

Open questions attached to this trait. Seeded by just knowledge-gap-scan and curated; see the corpus-wide index.

Resolve Brc233 native host breadth, exact sensitive-phage breadth, molecular output, and DefenseFinder HMM/rules coverage before minting narrower Brc233 mechanism children.

KNOWLEDGE GAP OPEN brc233-defensefinder-model-gap · raised by codex · 2026-10-01

Attached to causal_graphs#brc233_integron_cassette_confers_phage_resistance

Kieffer et al. identify brc233 as a bacteriophage-resistance integron cassette, and the pinned DefenseFinder article registry maps the gcu233 source key to the same Science article. The pinned HMM inventory and rules table have no exact gcu233 or Brc233 row, so the first-pass record leaves native-host breadth, profile boundaries, exact phage target breadth, and direct molecular output unresolved.

Evidence

Curation history

  1. · MINTED_TRAITMECH_ID · codex

    Minted Brc233 system as a DOI- and DefenseFinder-backed GENOMICS TraitRecord under phage defense system after an ignored-and-hidden duplicate review found no exact live TraitMech, METPO, history, or prior proposal record; kept the graph at BRiC integron-cassette level because the pinned DefenseFinder article registry names gcu233 but the pinned HMM inventory and rules table have no exact Brc233 row; the replacement placeholder is reserved in proposals/metpo_traitmech_v395.

  2. · REVIEW_CANONICAL_EXAMPLE_EVIDENCE_GAP · codex

    Reviewed Brc233 system during initial curation and left canonical_examples empty because the sources support heterologous gcu233/Brc233 phage-resistance assays and a DefenseFinder gcu233 article row, but not a direct named native microbial isolate exemplar with experimentally verified endogenous Brc233 activity. No paid research was used.