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Brc59 system

traitmech:000525 · CLASS · PROPOSED

A phage defense system in which an organism possesses a Brc59 bacteriophage-resistance integron cassette that supports growth during bacteriophage challenge.

Trait evidence (6)

  • DOI:10.1126/science.ads0915
    brc59 L 44.7 49.6 87.3 98.6 7.6

    Manual transcription of the Supplementary Fig. S2 Brc59 low-MOI E. coli IJ1862 heatmap row; the visible cells show reduced inhibition, including 44.7% for MS2, 49.6% for T4, 87.3% for F1, 98.6% for T7, and 7.6% for F13.

  • DOI:10.1126/science.ads0915
    brc59 H 12.8 75.8 80.3 60.9

    Manual transcription of the Supplementary Fig. S2 Brc59 high-MOI E. coli IJ1862 heatmap row; the visible cells show reduced inhibition, including 12.8% for MS2, 75.8% for F1, 80.3% for HK544, and 60.9% for F13.

  • DOI:10.1126/science.ads0915
    defense profiles of identified Bacteriophage Resistance integron Cassettes (BRiCs) against a panel of different phages

    Kieffer et al. classify positive integron cassettes as BRiCs and compare their defense profiles across a phage panel.

  • DOI:10.1126/science.ads0915
    brc59 brc128 brc135 brc142 brc167.2 brc167

    Kieffer et al. include brc59 among identified BRiCs with predicted attC secondary structures.

  • https://www.science.org/doi/suppl/10.1126/science.ads0915/suppl_file/science.ads0915_sm.pdf
    C800 pMBA brc59 E. coli IJ1862

    Kieffer et al.'s supplementary construct table lists pMBA brc59 in E. coli IJ1862 for high/low-MOI phage-panel testing.

  • https://www.science.org/doi/suppl/10.1126/science.ads0915/suppl_file/science.ads0915_sm.pdf
    C810 pMBA brc59 K. pneumoniae KP5

    Kieffer et al.'s supplementary construct table lists the pMBA brc59 plasmid introduced into Klebsiella pneumoniae KP5.

Brc59 BRiCs confer phage resistance

Conservative system-level sketch linking a Brc59 bacteriophage-resistance integron cassette to phage resistance and Brc59 system possession.

NONMECHANISTIC · The graph captures Brc59 at the integron-cassette and phage-resistance-output level without asserting the native host range, exact sensitive-phage breadth outside the reported panels, direct molecular substrate, a normalized gcu59 source key, or a DefenseFinder article/HMM/rules profile absent from the pinned snapshot.

Brc59 BRiCs confer phage resistance Interactive directed graph showing evidence-backed causal relationships for Brc59 system.

Edge evidence

  • Brc59 integron cassette contributes to Brc59 phage resistance RO:0002326

    The Brc59 integron cassette contributes to phage resistance during phage-panel assays.

    • DOI:10.1126/science.ads0915 brc59 L 44.7 49.6 87.3 98.6 7.6 Manual transcription of the Supplementary Fig. S2 Brc59 low-MOI E. coli IJ1862 heatmap row; the visible cells show reduced inhibition, including 44.7% for MS2, 49.6% for T4, 87.3% for F1, 98.6% for T7, and 7.6% for F13.
    • DOI:10.1126/science.ads0915 brc59 H 12.8 75.8 80.3 60.9 Manual transcription of the Supplementary Fig. S2 Brc59 high-MOI E. coli IJ1862 heatmap row; the visible cells show reduced inhibition, including 12.8% for MS2, 75.8% for F1, 80.3% for HK544, and 60.9% for F13.
  • Brc59 phage resistance confers Brc59 system METPO:2007700

    Brc59-linked phage resistance realizes the Brc59 system possession trait.

    • DOI:10.1126/science.ads0915 brc59 L 44.7 49.6 87.3 98.6 7.6 Manual transcription of the Supplementary Fig. S2 Brc59 low-MOI E. coli IJ1862 heatmap row; the visible cells show reduced inhibition, including 44.7% for MS2, 49.6% for T4, 87.3% for F1, 98.6% for T7, and 7.6% for F13.
    • DOI:10.1126/science.ads0915 brc59 H 12.8 75.8 80.3 60.9 Manual transcription of the Supplementary Fig. S2 Brc59 high-MOI E. coli IJ1862 heatmap row; the visible cells show reduced inhibition, including 12.8% for MS2, 75.8% for F1, 80.3% for HK544, and 60.9% for F13.
    • DOI:10.1126/science.ads0915 defense profiles of identified Bacteriophage Resistance integron Cassettes (BRiCs) against a panel of different phages Kieffer et al. classify positive integron cassettes as BRiCs and compare their defense profiles across a phage panel.
  • Brc59 system is a phage defense system rdfs:subClassOf

    Brc59 system possession is a phage-defense-system trait.

    • DOI:10.1126/science.ads0915 defense profiles of identified Bacteriophage Resistance integron Cassettes (BRiCs) against a panel of different phages Kieffer et al. classify positive integron cassettes as BRiCs and compare their defense profiles across a phage panel.
    • DOI:10.1126/science.ads0915 brc59 brc128 brc135 brc142 brc167.2 brc167 Kieffer et al. include brc59 among identified BRiCs with predicted attC secondary structures.

Provenance

Identifier source
TraitMech local identifier
Definition source
DOI:10.1126/science.ads0915

Parent traits (1)

Synonyms (1)

  • brc59 RELATED_SYNONYM · DOI:10.1126/science.ads0915

kg-microbe context

No kg-microbe node embedding matched this record in the 2026-04-25 deepwalk.

Discussions and Knowledge Gaps (1)

Open questions attached to this trait. Seeded by just knowledge-gap-scan and curated; see the corpus-wide index.

Resolve Brc59 native host breadth, exact sensitive-phage breadth, molecular output, normalized source-key naming, and DefenseFinder article/HMM/rules coverage before minting narrower Brc59 mechanism children.

KNOWLEDGE GAP OPEN brc59-defensefinder-model-gap · raised by codex · 2026-10-01

Attached to causal_graphs#brc59_integron_cassette_confers_phage_resistance

Kieffer et al. identify brc59 as a bacteriophage-resistance integron cassette. The pinned DefenseFinder article registry, HMM inventory, and rules table have no exact gcu59 or Brc59 row, so the first-pass record leaves native-host breadth, profile boundaries, exact phage target breadth, direct molecular output, and normalized gcu59 source-key status unresolved.

Evidence

Curation history

  1. · MINTED_TRAITMECH_ID · codex

    Minted Brc59 system as a DOI-backed GENOMICS TraitRecord under phage defense system after an ignored-and-hidden duplicate review ruled out same-scope live TraitMech, METPO, history, and prior proposal collisions after reviewing exact Brc59/brc59/gcu59 hits; kept the graph at BRiC integron-cassette level because the pinned DefenseFinder article registry, HMM inventory, and rules table have no exact Brc59 row; the replacement placeholder is reserved in proposals/metpo_traitmech_v402.

  2. · REVIEW_CANONICAL_EXAMPLE_EVIDENCE_GAP · codex

    Reviewed Brc59 system during initial curation and left canonical_examples empty because the sources support heterologous brc59 phage-resistance assays but not a direct named native microbial isolate exemplar with experimentally verified endogenous Brc59 activity. No paid research was used.