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BrcWGS21 system

traitmech:000520 · CLASS · PROPOSED

A phage defense system in which an organism possesses a gcuWGS21/BrcWGS21 bacteriophage-resistance integron cassette that supports growth during bacteriophage challenge.

Trait evidence (7)

  • DOI:10.1126/science.ads0915
    brcWGS21 L 88.6 60.5 93.4 77.2 25.6 0 93.4

    Manual transcription of the Supplementary Fig. S2 BrcWGS21 low-MOI heatmap row; the visible cells show reduced inhibition, including 88.6% for MS2, 60.5% for T7, 93.4% for HK544, 77.2% for F13, 25.6% for Vs1, 0% for Px4, and 93.4% for Px5.

  • DOI:10.1126/science.ads0915
    brcWGS21 H 95.6 98.2 84.9 98.0 24.9 78.4

    Manual transcription of the Supplementary Fig. S2 BrcWGS21 high-MOI heatmap row; the visible cells show reduced inhibition, including 95.6% for MS2, 98.2% for phi80, 84.9% for HK544, 98.0% for F13, 24.9% for Vs1, and 78.4% for Px4.

  • DOI:10.1126/science.ads0915
    defense profiles of identified Bacteriophage Resistance integron Cassettes (BRiCs) against a panel of different phages

    Kieffer et al. classify positive integron cassettes as BRiCs and compare their defense profiles across a phage panel.

  • DOI:10.1126/science.ads0915
    brc113 brc217 brc76 brcWGS21 brc22 brc23

    Kieffer et al. include brcWGS21 among identified BRiCs with predicted attC secondary structures.

  • DOI:10.1126/science.ads0915
    C935 pMBA brcWGS21 E. coli IJ1862

    Kieffer et al.'s supplementary construct table lists pMBA brcWGS21 in E. coli IJ1862 for high/low-MOI phage-panel testing.

  • DOI:10.1126/science.ads0915
    C941 pMBA brcWGS21 K. pneumoniae KP5

    Kieffer et al.'s supplementary construct table lists the pMBA brcWGS21 plasmid introduced into Klebsiella pneumoniae KP5.

  • https://raw.githubusercontent.com/mdmparis/defense-finder-models/afb0e5a8b466be53586b13266f5d38d98c3ac268/List_system_article.md
    | gcuWGS21 | 10\.1126/science\.ads0915 | Mobile integrons encode phage defense systems |

    The pinned DefenseFinder article registry maps the gcuWGS21 source key to the Kieffer et al. mobile-integron BRiC paper.

BrcWGS21 BRiCs confer phage resistance

Conservative system-level sketch linking a gcuWGS21/BrcWGS21 bacteriophage-resistance integron cassette to phage resistance and BrcWGS21 system possession.

NONMECHANISTIC · The graph captures BrcWGS21 at the integron-cassette and phage-resistance-output level without asserting the native host range, exact sensitive-phage breadth outside the reported panels, direct molecular substrate, or a DefenseFinder HMM/rules profile absent from the pinned snapshot.

BrcWGS21 BRiCs confer phage resistance Interactive directed graph showing evidence-backed causal relationships for BrcWGS21 system.

Edge evidence

  • BrcWGS21 integron cassette contributes to BrcWGS21 phage resistance RO:0002326

    The gcuWGS21/BrcWGS21 integron cassette contributes to phage resistance during phage-panel assays.

    • DOI:10.1126/science.ads0915 brcWGS21 L 88.6 60.5 93.4 77.2 25.6 0 93.4 Manual transcription of the Supplementary Fig. S2 BrcWGS21 low-MOI heatmap row; the visible cells show reduced inhibition, including 88.6% for MS2, 60.5% for T7, 93.4% for HK544, 77.2% for F13, 25.6% for Vs1, 0% for Px4, and 93.4% for Px5.
    • DOI:10.1126/science.ads0915 brcWGS21 H 95.6 98.2 84.9 98.0 24.9 78.4 Manual transcription of the Supplementary Fig. S2 BrcWGS21 high-MOI heatmap row; the visible cells show reduced inhibition, including 95.6% for MS2, 98.2% for phi80, 84.9% for HK544, 98.0% for F13, 24.9% for Vs1, and 78.4% for Px4.
  • BrcWGS21 phage resistance confers BrcWGS21 system METPO:2007700

    BrcWGS21-linked phage resistance realizes the BrcWGS21 system possession trait.

    • DOI:10.1126/science.ads0915 brcWGS21 L 88.6 60.5 93.4 77.2 25.6 0 93.4 Manual transcription of the Supplementary Fig. S2 BrcWGS21 low-MOI heatmap row; the visible cells show reduced inhibition, including 88.6% for MS2, 60.5% for T7, 93.4% for HK544, 77.2% for F13, 25.6% for Vs1, 0% for Px4, and 93.4% for Px5.
    • DOI:10.1126/science.ads0915 brcWGS21 H 95.6 98.2 84.9 98.0 24.9 78.4 Manual transcription of the Supplementary Fig. S2 BrcWGS21 high-MOI heatmap row; the visible cells show reduced inhibition, including 95.6% for MS2, 98.2% for phi80, 84.9% for HK544, 98.0% for F13, 24.9% for Vs1, and 78.4% for Px4.
    • DOI:10.1126/science.ads0915 defense profiles of identified Bacteriophage Resistance integron Cassettes (BRiCs) against a panel of different phages Kieffer et al. classify positive integron cassettes as BRiCs and compare their defense profiles across a phage panel.
  • BrcWGS21 system is a phage defense system rdfs:subClassOf

    BrcWGS21 system possession is a phage-defense-system trait.

Provenance

Identifier source
TraitMech local identifier
Definition source
DOI:10.1126/science.ads0915

Parent traits (1)

Synonyms (2)

  • gcuWGS21 RELATED_SYNONYM · https://raw.githubusercontent.com/mdmparis/defense-finder-models/afb0e5a8b466be53586b13266f5d38d98c3ac268/List_system_article.md
  • brcWGS21 RELATED_SYNONYM · DOI:10.1126/science.ads0915

kg-microbe context

No kg-microbe node embedding matched this record in the 2026-04-25 deepwalk.

Discussions and Knowledge Gaps (1)

Open questions attached to this trait. Seeded by just knowledge-gap-scan and curated; see the corpus-wide index.

Resolve BrcWGS21 native host breadth, exact sensitive-phage breadth, molecular output, and DefenseFinder HMM/rules coverage before minting narrower BrcWGS21 mechanism children.

KNOWLEDGE GAP OPEN brcwgs21-defensefinder-model-gap · raised by codex · 2026-10-01

Attached to causal_graphs#brcwgs21_integron_cassette_confers_phage_resistance

Kieffer et al. identify brcWGS21 as a bacteriophage-resistance integron cassette, and the pinned DefenseFinder article registry maps the gcuWGS21 source key to the same Science article. The pinned HMM inventory and rules table have no exact gcuWGS21 or BrcWGS21 row, so the first-pass record leaves native-host breadth, profile boundaries, exact phage target breadth, and direct molecular output unresolved.

Evidence

Curation history

  1. · MINTED_TRAITMECH_ID · codex

    Minted BrcWGS21 system as a DOI- and DefenseFinder-backed GENOMICS TraitRecord under phage defense system after an ignored-and-hidden duplicate review found no exact live TraitMech, METPO, history, or prior proposal record; kept the graph at BRiC integron-cassette level because the pinned DefenseFinder article registry names gcuWGS21 but the pinned HMM inventory and rules table have no exact BrcWGS21 row; the replacement placeholder is reserved in proposals/metpo_traitmech_v397.

  2. · REVIEW_CANONICAL_EXAMPLE_EVIDENCE_GAP · codex

    Reviewed BrcWGS21 system during initial curation and left canonical_examples empty because the sources support heterologous gcuWGS21/BrcWGS21 phage-resistance assays and a DefenseFinder gcuWGS21 article row, but not a direct named native microbial isolate exemplar with experimentally verified endogenous BrcWGS21 activity. No paid research was used.