HEC-08 system

traitmech:000479 · CLASS · PROPOSED

A phage defense system in which an organism possesses the single-gene HEC-08 Hma-embedded candidate locus, encoding a higher eukaryotes and prokaryotes nucleotide-binding domain-containing protein, that can reduce bacteriophage plaquing.

Trait evidence (4)

DOI:10.1101/2024.01.29.577857
RelE (HEC-07), higher eukaryotes and prokaryotes nucleotide-binding (HEPN) (HEC-08) and Nedd4-BP1/YacP nuclease (NYN) (HEC-09) domain-containing proteins

Payne et al. identify HEC-08 as a HEPN-domain Hma-embedded candidate system.
DOI:10.1101/2024.01.29.577857
these were able to reduce the EOP of at least two phages

Payne et al. support reduced plaquing for the active Hma-embedded candidate systems after excluding inactive HEC-01 and HEC-09 candidates.
https://raw.githubusercontent.com/mdmparis/defense-finder-models/afb0e5a8b466be53586b13266f5d38d98c3ac268/List_system_article.md
| HEC-08 | 10\.1101/2024\.01\.29\.577857 | New antiviral defences are genetically embedded within prokaryotic immune systems |

The pinned DefenseFinder article registry maps the HEC-08 source key to the Payne et al. Hma-embedded-candidate preprint.
https://raw.githubusercontent.com/mdmparis/defense-finder-models/afb0e5a8b466be53586b13266f5d38d98c3ac268/Liste_hmm_system.md
| HEC-08__HEC-08 | | HEC-08 | Custom | 200 |

The pinned DefenseFinder HMM inventory records HEC-08__HEC-08 as a custom HEC-08 profile.

HEC-08 loci reduce bacteriophage plaquing

Conservative system-level sketch linking the single-gene HEC-08 locus to reduced bacteriophage plaquing and HEC-08 system possession.

NONMECHANISTIC · The graph captures HEC-08 at candidate locus and anti-phage-output level without asserting native host breadth, the direct viral trigger or substrate, exact HEPN-domain molecular output, or a DefenseFinder detection rule absent from the pinned rules table.

Edge evidence

HEC-08 locus contributes to reduced bacteriophage plaquing RO:0002326
The HEC-08 locus contributes to reduced bacteriophage plaquing as a HEPN-domain Hma-embedded candidate.
- DOI:10.1101/2024.01.29.577857 RelE (HEC-07), higher eukaryotes and prokaryotes nucleotide-binding (HEPN) (HEC-08) and Nedd4-BP1/YacP nuclease (NYN) (HEC-09) domain-containing proteins Payne et al. identify HEC-08 among the HEPN-domain Hma-embedded candidates.
- https://raw.githubusercontent.com/mdmparis/defense-finder-models/afb0e5a8b466be53586b13266f5d38d98c3ac268/Liste_hmm_system.md | HEC-08__HEC-08 | | HEC-08 | Custom | 200 | The pinned DefenseFinder HMM inventory records HEC-08__HEC-08 as a custom HEC-08 profile.
reduced bacteriophage plaquing confers HEC-08 system METPO:2007700
HEC-08-mediated plaquing reduction realizes the HEC-08 system trait.
- DOI:10.1101/2024.01.29.577857 these were able to reduce the EOP of at least two phages Payne et al. support reduced efficiency of plaquing for the active HEC systems.
HEC-08 system is a phage defense system rdfs:subClassOf
HEC-08 system possession is a phage-defense-system trait.
- https://raw.githubusercontent.com/mdmparis/defense-finder-models/afb0e5a8b466be53586b13266f5d38d98c3ac268/List_system_article.md | HEC-08 | 10\.1101/2024\.01\.29\.577857 | New antiviral defences are genetically embedded within prokaryotic immune systems | The pinned DefenseFinder article registry maps the HEC-08 source key to the Payne et al. Hma-embedded-candidate preprint.

Provenance

Identifier source: TraitMech local identifier
Definition source: DOI:10.1101/2024.01.29.577857

Parent traits (1)

phage defense system traitmech:000209

Synonyms (2)

HEC-08 EXACT_SYNONYM · DOI:10.1101/2024.01.29.577857
HEC-08__HEC-08 RELATED_SYNONYM · https://raw.githubusercontent.com/mdmparis/defense-finder-models/afb0e5a8b466be53586b13266f5d38d98c3ac268/Liste_hmm_system.md

kg-microbe context

No kg-microbe node embedding matched this record in the 2026-04-25 deepwalk.

Discussions and Knowledge Gaps (1)

Open questions attached to this trait. Seeded by just knowledge-gap-scan and curated; see the corpus-wide index.

Resolve HEC-08 native host breadth, sensitive-phage breadth, HEPN-domain molecular output, and rule-level detection criteria before minting narrower HEC-08 mechanism children.

KNOWLEDGE GAP OPEN hec-08-defensefinder-model-gap · raised by codex · 2026-09-30

Attached to causal_graphs#hec_08_locus_reduces_phage_plaquing

Payne et al. support HEC-08 as a single-gene Hma-embedded candidate anti-phage system encoding a HEPN-domain protein, and the pinned DefenseFinder HMM inventory records one HEC-08 profile row. The pinned rules table has no HEC-08 row, and the first-pass record does not yet resolve natural host breadth, phage target breadth, or the direct molecular output.

Evidence

DOI:10.1101/2024.01.29.577857
RelE (HEC-07), higher eukaryotes and prokaryotes nucleotide-binding (HEPN) (HEC-08) and Nedd4-BP1/YacP nuclease (NYN) (HEC-09) domain-containing proteins
https://raw.githubusercontent.com/mdmparis/defense-finder-models/afb0e5a8b466be53586b13266f5d38d98c3ac268/List_system_article.md
| HEC-08 | 10\.1101/2024\.01\.29\.577857 | New antiviral defences are genetically embedded within prokaryotic immune systems |
https://raw.githubusercontent.com/mdmparis/defense-finder-models/afb0e5a8b466be53586b13266f5d38d98c3ac268/Liste_hmm_system.md
| HEC-08__HEC-08 | | HEC-08 | Custom | 200 |
https://raw.githubusercontent.com/mdmparis/defense-finder-models/afb0e5a8b466be53586b13266f5d38d98c3ac268/DefenseFinder_rules.tsv

Curation history

2026-09-30T01:34:07Z · MINTED_TRAITMECH_ID · codex
Minted HEC-08 system as a DOI-backed GENOMICS TraitRecord under phage defense system after an ignored-and-hidden duplicate review found no exact live TraitMech, METPO, history, or prior proposal record; kept the graph at single-gene candidate-locus level because the pinned DefenseFinder HEC-08 HMM row is not backed by a pinned rules row; the replacement placeholder is reserved in proposals/metpo_traitmech_v356.
2026-09-30T01:34:08Z · REVIEW_CANONICAL_EXAMPLE_EVIDENCE_GAP · codex
Reviewed HEC-08 system during canonical-example issue 444 enforcement and left canonical_examples empty because the sources support heterologous efficiency-of-plaquing assays and a DefenseFinder HEC-08 HMM row, but not a direct named native microbial isolate exemplar with experimentally verified endogenous HEC-08 activity. No paid research was used.