TraitMech

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MksBEFG system

traitmech:000526 · CLASS · PROPOSED

A Wadjet system in which an organism possesses an MksBEFG derivative SMC locus encoding an MksBEF ATPase core and an MksG nuclease that degrades plasmid DNA.

Trait evidence (5)

  • DOI:10.1093/nar/gkad130
    The MksG nuclease is the executing part of the bacterial plasmid defense system MksBEFG

    Weiss et al. describe MksBEFG as a bacterial plasmid-defense system and identify MksG as its executing nuclease component.

  • DOI:10.1093/nar/gkad130
    MksG is a nuclease that degrades plasmid DNA

    Weiss et al. support plasmid-DNA degradation by the MksG nuclease of the MksBEFG system.

  • DOI:10.1093/nar/gkad130
    Introduction of plasmids results in an increase in DNA bound MksG, indicating an activation of the system in vivo

    Weiss et al. support plasmid-responsive activation of the Corynebacterium glutamicum MksBEFG system in vivo.

  • DOI:10.1093/nar/gkad130
    The MksBEF subunits exhibit an ATPase cycle in vitro

    Weiss et al. support ATPase cycling by the C. glutamicum MksBEF core.

  • DOI:10.1016/j.molcel.2022.11.015
    Wadjet systems (JetABCD/MksBEFG/EptABCD) are derivative SMC complexes with roles in bacterial immunity against selfish DNA

    Liu et al. support MksBEFG as a Wadjet-family derivative SMC complex related to JetABCD and EptABCD.

MksBEFG systems degrade plasmid DNA

Conservative system-level sketch linking MksBEFG loci to MksG-dependent plasmid DNA degradation and Wadjet system possession.

NONMECHANISTIC · The graph captures the MksBEFG subfamily at locus and plasmid-degradation-output level without asserting the exact MksBEF ATPase-to-MksG coupling step, plasmid substrate breadth, cellular MksG loading path, or accession-level protein examples.

MksBEFG systems degrade plasmid DNA Interactive directed graph showing evidence-backed causal relationships for MksBEFG system.

Edge evidence

  • MksBEFG locus contributes to MksBEF ATPase cycle RO:0002326

    MksBEFG loci encode the MksBEF ATPase core.

    • DOI:10.1093/nar/gkad130 The MksBEF subunits exhibit an ATPase cycle in vitro Weiss et al. support ATPase cycling by the C. glutamicum MksBEF core.
  • MksBEFG locus contributes to MksG plasmid DNA degradation RO:0002326

    MksBEFG loci encode the MksG nuclease that degrades plasmid DNA.

    • DOI:10.1093/nar/gkad130 The MksG nuclease is the executing part of the bacterial plasmid defense system MksBEFG Weiss et al. describe MksBEFG as a bacterial plasmid-defense system and identify MksG as its executing nuclease component.
    • DOI:10.1093/nar/gkad130 MksG is a nuclease that degrades plasmid DNA Weiss et al. support plasmid-DNA degradation by the MksG nuclease of the MksBEFG system.
  • MksG plasmid DNA degradation mitigates plasmid transformation METPO:2007407

    MksG-dependent plasmid DNA degradation restricts incoming plasmid DNA.

    • DOI:10.1093/nar/gkad130 MksG is a nuclease that degrades plasmid DNA Weiss et al. support plasmid-DNA degradation by the MksG nuclease of the MksBEFG system.
    • DOI:10.1093/nar/gkad130 Introduction of plasmids results in an increase in DNA bound MksG, indicating an activation of the system in vivo Weiss et al. support plasmid-responsive activation of the Corynebacterium glutamicum MksBEFG system in vivo.
  • MksG plasmid DNA degradation confers MksBEFG system METPO:2007700

    Plasmid DNA degradation realizes the MksBEFG anti-plasmid defense trait.

    • DOI:10.1093/nar/gkad130 The MksG nuclease is the executing part of the bacterial plasmid defense system MksBEFG Weiss et al. describe MksBEFG as a bacterial plasmid-defense system and identify MksG as its executing nuclease component.
  • MksBEFG system is a Wadjet system rdfs:subClassOf

    MksBEFG system possession is a Wadjet system trait.

    • DOI:10.1016/j.molcel.2022.11.015 Wadjet systems (JetABCD/MksBEFG/EptABCD) are derivative SMC complexes with roles in bacterial immunity against selfish DNA Liu et al. support MksBEFG as a Wadjet-family derivative SMC complex related to JetABCD and EptABCD.

Provenance

Identifier source
TraitMech local identifier
Definition source
DOI:10.1093/nar/gkad130

Parent traits (1)

Synonyms (1)

  • MksBEFG RELATED_SYNONYM · DOI:10.1093/nar/gkad130

kg-microbe context

No kg-microbe node embedding matched this record in the 2026-04-25 deepwalk.

Canonical examples (1)

Organisms cited as exemplars of this trait. Taxon ids are NCBITaxon and link out to the NCBI record.

  • Corynebacterium glutamicum NCBITaxon:1718 DOI:10.1093/nar/gkad130 Weiss et al. investigated the Corynebacterium glutamicum MksBEFG complex and showed that plasmid introduction increased DNA-bound MksG in vivo.

Discussions and Knowledge Gaps (1)

Open questions attached to this trait. Seeded by just knowledge-gap-scan and curated; see the corpus-wide index.

Resolve MksBEFG plasmid substrate breadth, MksBEF ATPase-to-MksG coupling, MksG activation state, and accession-level protein examples before minting narrower MksBEFG mechanism or component traits.

KNOWLEDGE GAP OPEN mksbefg-activation-and-substrate-gap · raised by codex · 2026-10-01

Attached to causal_graphs#mksbefg_degrades_plasmid_dna

Weiss et al. support MksBEFG as a bacterial plasmid-defense system with an MksBEF ATPase core and MksG plasmid-DNA-degradation nuclease, and Liu et al. place MksBEFG in the broader Wadjet derivative-SMC family. This first-pass record leaves exact plasmid substrate breadth, MksBEF-to-MksG coupling, cellular MksG loading, and accession-level component examples unresolved.

Evidence

Curation history

  1. · MINTED_TRAITMECH_ID · codex

    Minted MksBEFG system as a DOI-backed GENOMICS TraitRecord under the Wadjet system parent after an ignored-and-hidden duplicate review found no exact same-scope live TraitMech, METPO, history, or prior proposal record; the replacement placeholder is reserved in proposals/metpo_traitmech_v403.