NixI system

Edge evidence

• NixI locus contributes to ICP1 genome cleavage RO:0002326

  The PLE-encoded NixI nicking endonuclease is necessary for cleavage of ICP1 DNA in vivo, and DefenseFinder models NixI with the mandatory NixI__NixI profile.

  • DOI:10.1093/nar/gkac002 Together, these data show that PLE encodes a nicking endonuclease, NixI, that is necessary for cleavage of ICP1’s genome in vivo and that shows in vitro specificity for sequences found in ICP1’s genome LeGault et al. support NixI as the PLE-encoded nicking endonuclease responsible for ICP1 genome cleavage.
  • https://raw.githubusercontent.com/mdmparis/defense-finder-models/afb0e5a8b466be53586b13266f5d38d98c3ac268/DefenseFinder_rules.tsv NixI NixI 1 1 NixI__NixI NixI__Stix The DefenseFinder rules table models NixI with the NixI__NixI profile as its mandatory component and an optional NixI__Stix profile.
  • https://raw.githubusercontent.com/mdmparis/defense-finder-models/afb0e5a8b466be53586b13266f5d38d98c3ac268/Liste_hmm_system.md | NixI__NixI | NixI__NixI | NixI | Custom | 20 | The DefenseFinder HMM inventory records NixI__NixI under the NixI model namespace.
• NixI locus contributes to ICP1 replication inhibition RO:0002326

  NixI inhibits ICP1 replication and decreases ICP1 virion production.

  • DOI:10.1093/nar/gkac002 Together, these data demonstrate that nixI is a potent inhibitor of ICP1 replication, resulting in decreased virion production. LeGault et al. show that NixI is a potent inhibitor of ICP1 replication.
  • DOI:10.1093/nar/gkac002 NixI, however, is sufficient to limit ICP1 progeny production in a single round of infection LeGault et al. show that NixI is sufficient to limit ICP1 progeny production.
• ICP1 genome cleavage contributes to ICP1 replication inhibition RO:0002326

  NixI-dependent cleavage of the ICP1 genome contributes to inhibition of ICP1 replication.

  • DOI:10.1093/nar/gkac002 Here, we characterize a PLE-encoded nuclease, NixI, that blocks phage development likely by nicking ICP1’s genome as it transitions to RCR LeGault et al. connect NixI's nicking of ICP1 DNA to blocked phage development during the transition to rolling-circle replication.
  • DOI:10.1093/nar/gkac002 Together, these data show that PLE encodes a nicking endonuclease, NixI, that is necessary for cleavage of ICP1’s genome in vivo and that shows in vitro specificity for sequences found in ICP1’s genome LeGault et al. support NixI as the PLE-encoded nicking endonuclease responsible for ICP1 genome cleavage.
• ICP1 replication inhibition confers NixI system METPO:2007700

  NixI-dependent ICP1 replication inhibition realizes the NixI system trait.

  • DOI:10.1093/nar/gkac002 Here, we characterize a PLE-encoded nuclease, NixI, that blocks phage development likely by nicking ICP1’s genome as it transitions to RCR LeGault et al. characterize NixI as a PLE-encoded nuclease blocking ICP1 phage development.
  • https://raw.githubusercontent.com/mdmparis/defense-finder-models/afb0e5a8b466be53586b13266f5d38d98c3ac268/List_system_article.md NixI | 10\.1101/2021\.07\.12\.452122 | A phage parasite deploys a nicking nuclease effector to inhibit replication of its viral host The DefenseFinder article registry maps the named NixI model namespace to the LeGault et al. PLE-encoded nicking-nuclease preprint, later published under DOI:10.1093/nar/gkac002.
• NixI system is a phage defense system rdfs:subClassOf

  NixI system possession is a phage-defense-system trait.

  • DOI:10.1093/nar/gkac002 Phage parasites can be considered host defense systems, as the parasite inhibits production of its viral host, benefiting the bacterial population LeGault et al. place phage parasites such as NixI-encoding PLE among host defense systems because they inhibit their viral hosts.