Rst_3HP system

Edge evidence

• Rst_3HP locus contributes to P1 phage resistance RO:0002326

  Rousset et al. linked a cloned three-gene P2-hotspot system to P1 protection, and DefenseFinder models Rst_3HP as a three-profile Hp1/Hp2/Hp3 system.

  • DOI:10.1016/j.chom.2022.02.018 The second system comprises three genes that have no clear predicted domain and protect against P1. Rousset et al. experimentally describe the unnamed three-gene P2-hotspot system that DefenseFinder tracks as Rst_3HP.
  • https://gitlab.pasteur.fr/mdm-lab/wiki/-/raw/ee7647d8/content/3.defense-systems/rst_3hp.md The Rst_3HP system is composed of 3 proteins: Hp1, Hp2 and, Hp3. These proteins do not have clear predicted domains but they confer resistance against the phage P1 in *Escherichia coli* E1114 :ref{doi=10.1016/j.chom.2022.02.018}. The pinned DefenseFinder wiki page names Rst_3HP as a three-protein system and links Hp1, Hp2, and Hp3 to P1 resistance in Escherichia coli E1114.
  • https://raw.githubusercontent.com/mdmparis/defense-finder-models/afb0e5a8b466be53586b13266f5d38d98c3ac268/DefenseFinder_rules.tsv Rst_3HP Rst_3HP 3 3 Rst_3HP__Hp1, Rst_3HP__Hp2, Rst_3HP__Hp3 The DefenseFinder rules table models Rst_3HP as a three-profile system requiring Rst_3HP__Hp1, Rst_3HP__Hp2, and Rst_3HP__Hp3.
  • https://raw.githubusercontent.com/mdmparis/defense-finder-models/afb0e5a8b466be53586b13266f5d38d98c3ac268/Liste_hmm_system.md | Rst_3HP__Hp1 | Rst_3HP__Hp1 | Rst_3HP | Custom | 67 | | Rst_3HP__Hp2 | Rst_3HP__Hp2 | Rst_3HP | Custom | 26 | | Rst_3HP__Hp3 | Rst_3HP__Hp3 | Rst_3HP | Custom | 20 | The DefenseFinder HMM inventory records Hp1, Hp2, and Hp3 custom profiles under Rst_3HP.
• P1 phage resistance confers Rst_3HP system METPO:2007700

  Rst_3HP-linked P1 resistance realizes the Rst_3HP system trait.

  • DOI:10.1016/j.chom.2022.02.018 The second system comprises three genes that have no clear predicted domain and protect against P1. Rousset et al. experimentally describe the unnamed three-gene P2-hotspot system that DefenseFinder tracks as Rst_3HP.
  • https://gitlab.pasteur.fr/mdm-lab/wiki/-/raw/ee7647d8/content/3.defense-systems/rst_3hp.md Rousset_2022[<a href='https://doi.org/10.1016/j.chom.2022.02.018'>Rousset et al., 2022</a>] --> Origin_0 Origin_0[Escherichia coli P2 loci <a href='https://ncbi.nlm.nih.gov/protein/WP_000508501.1'>WP_000508501.1</a>, <a href='https://ncbi.nlm.nih.gov/protein/WP_112026686.1'>WP_112026686.1</a>, <a href='https://ncbi.nlm.nih.gov/protein/WP_000756244.1'>WP_000756244.1</a>] --> Expressed_0[Escherichia coli] Expressed_0[Escherichia coli] ----> P1 The DefenseFinder experimental-validation diagram links Rousset et al. to an Escherichia coli P2-locus source expressed in Escherichia coli against P1.
  • https://gitlab.pasteur.fr/mdm-lab/wiki/-/raw/ee7647d8/content/3.defense-systems/rst_3hp.md subgraph Title4[Protects against] P1 The DefenseFinder experimental-validation diagram lists P1 in the Rst_3HP protects-against subgraph.
• Rst_3HP system is a phage defense system rdfs:subClassOf

  Rst_3HP system possession is a phage-defense-system trait.

  • DOI:10.1016/j.chom.2022.02.018 Phage resistance heatmaps of the validated defense systems show the median fold resistance of three independent replicates against a panel of eight phages The Figure 5 legend frames the cloned P2-hotspot hits, including the three-gene P1-restricting system, as validated defense systems in a replicated phage-resistance heatmap.
  • https://raw.githubusercontent.com/mdmparis/defense-finder-models/afb0e5a8b466be53586b13266f5d38d98c3ac268/List_system_article.md Rst_3HP | 10\.1101/2021\.01\.21\.427644 | Prophage-encoded hotspots of bacterial immune systems The DefenseFinder article registry maps the named Rst_3HP system to the Rousset et al. preprint.