Rst_DUF4238 system

traitmech:000374 · CLASS · PROPOSED

A phage defense system in which an organism possesses a single-gene Rst_DUF4238 locus represented by DefenseFinder as an Rst_DUF4238__DUF4238_Pers single-profile model and experimentally linked to strong resistance against phage T7.

Trait evidence (8)

DOI:10.1016/j.chom.2022.02.018
The first system consists of a single protein with a DUF4238 domain (whose molecular function is currently unknown) that provides strong resistance against T7.

Rousset et al. describe the one-protein DUF4238 candidate from the P2-encoded hotspot and connect it to strong T7 resistance.
DOI:10.1016/j.chom.2022.02.018
Phage resistance heatmaps of the validated defense systems show the median fold resistance of three independent replicates against a panel of eight phages

The Figure 5 legend frames the cloned P2-hotspot hits, including the DUF4238 system, as validated defense systems in a replicated phage-resistance heatmap.
https://gitlab.pasteur.fr/mdm-lab/wiki/-/raw/ee7647d8/content/3.defense-systems/rst_duf4238.md
Rst_DUF4238 is a single gene system found in a screen of phage and phage-satellites antiviral hotspots :ref{doi=10.1016/j.chom.2022.02.018}. It was shown to provide *E.coli* with a strong resistance against phage T7.

The pinned DefenseFinder wiki page names Rst_DUF4238 as a single-gene system and links it to strong T7 resistance.
https://gitlab.pasteur.fr/mdm-lab/wiki/-/raw/ee7647d8/content/3.defense-systems/rst_duf4238.md
As far as we are aware, the molecular mechanism is unknown.

The pinned DefenseFinder wiki page leaves the Rst_DUF4238 molecular mechanism unresolved.
https://gitlab.pasteur.fr/mdm-lab/wiki/-/raw/ee7647d8/content/3.defense-systems/rst_duf4238.md
The Rst_DUF4238 is composed of 1 protein: DUF4238.

The pinned DefenseFinder wiki page describes Rst_DUF4238 as a one-component DUF4238 system.
https://raw.githubusercontent.com/mdmparis/defense-finder-models/afb0e5a8b466be53586b13266f5d38d98c3ac268/List_system_article.md
Rst_DUF4238 | 10\.1101/2021\.01\.21\.427644 | Prophage-encoded hotspots of bacterial immune systems

The DefenseFinder article registry maps the named Rst_DUF4238 system to the Rousset et al. preprint.
https://raw.githubusercontent.com/mdmparis/defense-finder-models/afb0e5a8b466be53586b13266f5d38d98c3ac268/DefenseFinder_rules.tsv
Rst_DUF4238 Rst_DUF4238 1 1 Rst_DUF4238__DUF4238_Pers

The DefenseFinder rules table models Rst_DUF4238 as a single-profile system requiring Rst_DUF4238__DUF4238_Pers.
https://raw.githubusercontent.com/mdmparis/defense-finder-models/afb0e5a8b466be53586b13266f5d38d98c3ac268/Liste_hmm_system.md
| Rst_DUF4238__DUF4238_Pers | Rst_DUF4238__DUF4238_Pers | Rst_DUF4238 | Custom | 100 |

The DefenseFinder HMM inventory records Rst_DUF4238__DUF4238_Pers under Rst_DUF4238.

Rst_DUF4238 loci restrict T7 phage

Conservative system-level sketch linking the named Rst_DUF4238 single-gene locus model to strong T7 phage resistance.

NONMECHANISTIC · The graph captures Rst_DUF4238 as a named DefenseFinder single-profile phage-defense system while leaving native host breadth, the molecular activity of the DUF4238 component, exact DUF4238_Pers profile grounding, and the mechanism of T7 restriction unresolved.

Edge evidence

Rst_DUF4238 locus contributes to T7 phage resistance RO:0002326
Rousset et al. linked a cloned one-protein DUF4238 locus to strong T7 resistance, and DefenseFinder models Rst_DUF4238 as a single-profile DUF4238_Pers system.
- DOI:10.1016/j.chom.2022.02.018 The first system consists of a single protein with a DUF4238 domain (whose molecular function is currently unknown) that provides strong resistance against T7. Rousset et al. describe the one-protein DUF4238 candidate from the P2-encoded hotspot and connect it to strong T7 resistance.
- https://raw.githubusercontent.com/mdmparis/defense-finder-models/afb0e5a8b466be53586b13266f5d38d98c3ac268/DefenseFinder_rules.tsv Rst_DUF4238 Rst_DUF4238 1 1 Rst_DUF4238__DUF4238_Pers The DefenseFinder rules table models Rst_DUF4238 as a single-profile system requiring Rst_DUF4238__DUF4238_Pers.
- https://raw.githubusercontent.com/mdmparis/defense-finder-models/afb0e5a8b466be53586b13266f5d38d98c3ac268/Liste_hmm_system.md | Rst_DUF4238__DUF4238_Pers | Rst_DUF4238__DUF4238_Pers | Rst_DUF4238 | Custom | 100 | The DefenseFinder HMM inventory records Rst_DUF4238__DUF4238_Pers under Rst_DUF4238.
T7 phage resistance confers Rst_DUF4238 system METPO:2007700
Strong T7 resistance realizes the Rst_DUF4238 system trait.
- DOI:10.1016/j.chom.2022.02.018 The first system consists of a single protein with a DUF4238 domain (whose molecular function is currently unknown) that provides strong resistance against T7. Rousset et al. describe the one-protein DUF4238 candidate from the P2-encoded hotspot and connect it to strong T7 resistance.
- https://gitlab.pasteur.fr/mdm-lab/wiki/-/raw/ee7647d8/content/3.defense-systems/rst_duf4238.md Rst_DUF4238 is a single gene system found in a screen of phage and phage-satellites antiviral hotspots :ref{doi=10.1016/j.chom.2022.02.018}. It was shown to provide *E.coli* with a strong resistance against phage T7. The pinned DefenseFinder wiki page names Rst_DUF4238 as a single-gene system and links it to strong T7 resistance.
Rst_DUF4238 system is a phage defense system rdfs:subClassOf
Rst_DUF4238 system possession is a phage-defense-system trait.
- DOI:10.1016/j.chom.2022.02.018 Phage resistance heatmaps of the validated defense systems show the median fold resistance of three independent replicates against a panel of eight phages The Figure 5 legend frames the cloned P2-hotspot hits, including the DUF4238 system, as validated defense systems in a replicated phage-resistance heatmap.
- https://raw.githubusercontent.com/mdmparis/defense-finder-models/afb0e5a8b466be53586b13266f5d38d98c3ac268/List_system_article.md Rst_DUF4238 | 10\.1101/2021\.01\.21\.427644 | Prophage-encoded hotspots of bacterial immune systems The DefenseFinder article registry maps the named Rst_DUF4238 system to the Rousset et al. preprint.

Provenance

Identifier source: TraitMech local identifier
Definition source: DOI:10.1016/j.chom.2022.02.018

Parent traits (1)

phage defense system traitmech:000209

Synonyms (3)

Rst_DUF4238 RELATED_SYNONYM · https://raw.githubusercontent.com/mdmparis/defense-finder-models/afb0e5a8b466be53586b13266f5d38d98c3ac268/List_system_article.md
Rst_DUF4238__DUF4238_Pers RELATED_SYNONYM · https://raw.githubusercontent.com/mdmparis/defense-finder-models/afb0e5a8b466be53586b13266f5d38d98c3ac268/Liste_hmm_system.md
DUF4238_Pers RELATED_SYNONYM · https://gitlab.pasteur.fr/mdm-lab/wiki/-/raw/ee7647d8/content/3.defense-systems/rst_duf4238.md

kg-microbe context

No kg-microbe node embedding matched this record in the 2026-04-25 deepwalk.

Discussions and Knowledge Gaps (1)

Open questions attached to this trait. Seeded by just knowledge-gap-scan and curated; see the corpus-wide index.

Resolve Rst_DUF4238 native host breadth, accession-level DUF4238 proteins, and the molecular mechanism of T7 restriction before minting narrower Rst_DUF4238 mechanism children.

KNOWLEDGE GAP OPEN rst-duf4238-host-mechanism-gap · raised by codex · 2026-09-26

Attached to causal_graphs#rst_duf4238_locus_restricts_t7

Rousset et al. support a one-protein DUF4238 P2-hotspot system that provides strong T7 resistance when expressed in Escherichia coli, and DefenseFinder represents Rst_DUF4238 as a single-profile Rst_DUF4238__DUF4238_Pers model. This first system-level record leaves native host breadth, accession-level component grounding, the profile-to-activity mapping, and the T7 restriction mechanism unresolved.

Evidence

DOI:10.1016/j.chom.2022.02.018
The first system consists of a single protein with a DUF4238 domain (whose molecular function is currently unknown) that provides strong resistance against T7.
https://gitlab.pasteur.fr/mdm-lab/wiki/-/raw/ee7647d8/content/3.defense-systems/rst_duf4238.md
As far as we are aware, the molecular mechanism is unknown.
https://gitlab.pasteur.fr/mdm-lab/wiki/-/raw/ee7647d8/content/3.defense-systems/rst_duf4238.md
The Rst_DUF4238 is composed of 1 protein: DUF4238.
https://raw.githubusercontent.com/mdmparis/defense-finder-models/afb0e5a8b466be53586b13266f5d38d98c3ac268/DefenseFinder_rules.tsv
Rst_DUF4238 Rst_DUF4238 1 1 Rst_DUF4238__DUF4238_Pers

Curation history

2026-09-26T07:22:10Z · MINTED_TRAITMECH_ID · codex
Minted Rst_DUF4238 system as a DOI-backed GENOMICS TraitRecord under phage defense system after an ignored-and-hidden duplicate review found no exact live TraitMech, METPO, history, or prior proposal record; the replacement placeholder is reserved in proposals/metpo_traitmech_v251.
2026-09-26T07:32:10Z · REVIEW_CANONICAL_EXAMPLE_EVIDENCE_GAP · codex
Reviewed Rst_DUF4238 during canonical-example issue 444 enforcement and left canonical_examples empty because the sources support a cloned P2-hotspot DUF4238 locus assayed in Escherichia coli and a DefenseFinder RefSeq model example, but not a direct native host exemplar with experimental Rst_DUF4238 activity. No paid research was used.