Rst gop-beta-cII system

Edge evidence

• Rst gop-beta-cII locus contributes to lambda and P1 phage restriction RO:0002326

  Rousset et al. linked gop-beta-cII to lambda and P1 phage protection, and DefenseFinder models Rst_gop_beta_cll as a three-profile system.

  • DOI:10.1016/j.chom.2022.02.018 The well-characterized P4 satellite (NC_001609) carries at this position the non-essential genes gop, β, and cII, with gop and β likely acting as a toxin-antitoxin pair Rousset et al. describe the canonical P4 satellite gop, beta, and cII locus before testing it for anti-phage activity.
  • DOI:10.1016/j.chom.2022.02.018 When compared with a control vector encoding a green fluorescent protein (GFP), seven systems provided robust and reproducible resistance to at least one phage Rousset et al. cloned P4/P2 hotspot systems under native promoters and measured reproducible phage resistance against their coliphage panel.
  • https://raw.githubusercontent.com/mdmparis/defense-finder-models/afb0e5a8b466be53586b13266f5d38d98c3ac268/DefenseFinder_rules.tsv Rst_gop_beta_cll Rst_gop_beta_cll 3 3 Rst_gop_beta_cll__beta, Rst_gop_beta_cll__cll, Rst_gop_beta_cll__gop The DefenseFinder rules table models Rst_gop_beta_cll as a three-profile system requiring beta, cll, and gop profiles.
  • https://raw.githubusercontent.com/mdmparis/defense-finder-models/afb0e5a8b466be53586b13266f5d38d98c3ac268/Liste_hmm_system.md | Rst_gop_beta_cll__beta | Rst_gop_beta_cll__beta | Rst_gop_beta_cll | Custom | 20 | | Rst_gop_beta_cll__cll | Rst_gop_beta_cll__cll | Rst_gop_beta_cll | Custom | 20 | | Rst_gop_beta_cll__gop | Rst_gop_beta_cll__gop | Rst_gop_beta_cll | Custom | 20 | The DefenseFinder HMM inventory records custom beta, cll, and gop profiles under the Rst_gop_beta_cll system namespace.
• lambda and P1 phage restriction confers Rst gop-beta-cII system METPO:2007700

  Restriction of lambda and P1 phages realizes the Rst gop-beta-cII system trait.

  • DOI:10.1016/j.chom.2022.02.018 We report that gop-β-cII forms an anti-phage system that protects against λ and P1. Rousset et al. directly identify gop-beta-cII as an anti-phage system that protects against lambda and P1.
  • DOI:10.1016/j.chom.2022.02.018 When compared with a control vector encoding a green fluorescent protein (GFP), seven systems provided robust and reproducible resistance to at least one phage Rousset et al. cloned P4/P2 hotspot systems under native promoters and measured reproducible phage resistance against their coliphage panel.
• Rst gop-beta-cII system is a phage defense system rdfs:subClassOf

  Rst gop-beta-cII system possession is a phage-defense-system trait.

  • DOI:10.1016/j.chom.2022.02.018 P2-like phages and their parasitic P4-like satellites carry hotspots of genetic variation containing reservoirs of anti-phage systems Rousset et al. frame the P4-like satellite hotspot that contains gop-beta-cII as a reservoir of anti-phage systems.
  • https://raw.githubusercontent.com/mdmparis/defense-finder-models/afb0e5a8b466be53586b13266f5d38d98c3ac268/List_system_article.md Rst_gop_beta_cll | 10\.1101/2021\.01\.21\.427644 | Prophage-encoded hotspots of bacterial immune systems The DefenseFinder article registry maps the named Rst_gop_beta_cll system to the Rousset et al. preprint.