TraitMech

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Rst_HelicaseDUF2290 system

traitmech:000401 · CLASS · PROPOSED

A phage defense system in which an organism possesses a two-protein Rst_HelicaseDUF2290 locus represented by DefenseFinder as a two-profile model requiring Rst_HelicaseDUF2290__DUF2290 and Rst_HelicaseDUF2290__Helicase and experimentally linked to T7 protection when expressed in Escherichia coli.

Trait evidence (11)

Rst_HelicaseDUF2290 loci restrict T7

Conservative system-level sketch linking the named Rst_HelicaseDUF2290 two-gene locus model to T7 phage resistance.

NONMECHANISTIC · The graph captures Rst_HelicaseDUF2290 as a named DefenseFinder two-profile phage-defense system while leaving native host breadth, accession-level DUF2290 and helicase roles, the extra DUF2290_Pers profile, and the mechanism of T7 restriction unresolved.

Rst_HelicaseDUF2290 loci restrict T7 Interactive directed graph showing evidence-backed causal relationships for Rst_HelicaseDUF2290 system.

Edge evidence

  • Rst_HelicaseDUF2290 locus contributes to T7 phage resistance RO:0002326

    Rousset et al. linked an ATP-dependent helicase plus DUF2290 locus to T7 restriction, and DefenseFinder models Rst_HelicaseDUF2290 as a two-profile system.

  • T7 phage resistance confers Rst_HelicaseDUF2290 system METPO:2007700

    Rst_HelicaseDUF2290-linked T7 resistance realizes the Rst_HelicaseDUF2290 system trait.

    • DOI:10.1016/j.chom.2022.02.018 two systems that specifically inhibit the growth of phage T7: an ATP-dependent helicase associated with a DUF2290 protein and a HAD-like hydrolase associated with a transmembrane protein Rousset et al. experimentally linked an ATP-dependent helicase plus DUF2290 protein pair to specific inhibition of phage T7 growth.
    • https://gitlab.pasteur.fr/mdm-lab/wiki/-/raw/223cf269/content/3.defense-systems/rst_helicaseduf2290.md Rousset_2022[<a href='https://doi.org/10.1016/j.chom.2022.02.018'>Rousset et al., 2022</a>] --> Origin_0 Origin_0[Klebsiella pneumoniae P4 loci <a href='https://ncbi.nlm.nih.gov/protein/WP_046623503.1'>WP_046623503.1</a>, <a href='https://ncbi.nlm.nih.gov/protein/WP_046623504.1'>WP_046623504.1</a>] --> Expressed_0[Escherichia coli] Expressed_0[Escherichia coli] ----> T7 The DefenseFinder experimental-validation diagram links Rousset et al. to a Klebsiella pneumoniae P4-locus source expressed in Escherichia coli against T7.
    • https://gitlab.pasteur.fr/mdm-lab/wiki/-/raw/223cf269/content/3.defense-systems/rst_helicaseduf2290.md subgraph Title4[Protects against] T7 The DefenseFinder experimental-validation diagram lists T7 in the Rst_HelicaseDUF2290 protects-against subgraph.
  • Rst_HelicaseDUF2290 system is a phage defense system rdfs:subClassOf

    Rst_HelicaseDUF2290 system possession is a phage-defense-system trait.

Provenance

Identifier source
TraitMech local identifier
Definition source
https://gitlab.pasteur.fr/mdm-lab/wiki/-/raw/223cf269/content/3.defense-systems/rst_helicaseduf2290.md

Parent traits (1)

Synonyms (5)

  • Rst_HelicaseDUF2290 RELATED_SYNONYM · https://raw.githubusercontent.com/mdmparis/defense-finder-models/afb0e5a8b466be53586b13266f5d38d98c3ac268/List_system_article.md
  • Rst_HelicaseDUF2290__DUF2290 RELATED_SYNONYM · https://raw.githubusercontent.com/mdmparis/defense-finder-models/afb0e5a8b466be53586b13266f5d38d98c3ac268/Liste_hmm_system.md
  • Rst_HelicaseDUF2290__DUF2290_Pers RELATED_SYNONYM · https://raw.githubusercontent.com/mdmparis/defense-finder-models/afb0e5a8b466be53586b13266f5d38d98c3ac268/Liste_hmm_system.md
  • Rst_HelicaseDUF2290__Helicase RELATED_SYNONYM · https://raw.githubusercontent.com/mdmparis/defense-finder-models/afb0e5a8b466be53586b13266f5d38d98c3ac268/Liste_hmm_system.md
  • DUF2290_Pers RELATED_SYNONYM · https://gitlab.pasteur.fr/mdm-lab/wiki/-/raw/223cf269/content/3.defense-systems/rst_helicaseduf2290.md

kg-microbe context

No kg-microbe node embedding matched this record in the 2026-04-25 deepwalk.

Discussions and Knowledge Gaps (1)

Open questions attached to this trait. Seeded by just knowledge-gap-scan and curated; see the corpus-wide index.

Resolve Rst_HelicaseDUF2290 native host breadth, accession-level DUF2290/helicase roles, the DUF2290_Pers profile relationship, and the mechanism of T7 restriction before minting narrower Rst_HelicaseDUF2290 mechanism children.

KNOWLEDGE GAP OPEN rst-helicaseduf2290-host-mechanism-gap · raised by codex · 2026-09-27

Attached to causal_graphs#rst_helicaseduf2290_locus_restricts_t7

Rousset et al. support a two-gene ATP-dependent helicase plus DUF2290 P4-hotspot system that inhibits T7 growth, and DefenseFinder represents Rst_HelicaseDUF2290 as a required two-profile model. This first system-level record leaves native host breadth, profile-to-gene mapping, the role of the extra DUF2290_Pers HMM profile, the molecular activities of the DUF2290 and helicase components, and the T7 restriction mechanism unresolved.

Evidence

Curation history

  1. · MINTED_TRAITMECH_ID · codex

    Minted Rst_HelicaseDUF2290 system as a DOI/stable-URL-backed GENOMICS TraitRecord under phage defense system after an ignored-and-hidden duplicate review found no exact live TraitMech, METPO, history, or prior proposal record; the replacement placeholder is reserved in proposals/metpo_traitmech_v278.

  2. · REVIEW_CANONICAL_EXAMPLE_EVIDENCE_GAP · codex

    Reviewed Rst_HelicaseDUF2290 during canonical-example issue 444 enforcement and left canonical_examples empty because the sources support a cloned P4-hotspot helicase/DUF2290 locus assayed in Escherichia coli and a DefenseFinder RefSeq model example, but not a direct native microbial host exemplar with experimentally verified Rst_HelicaseDUF2290 activity. No paid research was used.

  3. · EDIT · codex

    Corrected the Rousset et al. replicated phage-resistance heatmap evidence for the P4-hotspot Rst_HelicaseDUF2290 system from the Figure 5 P2 median-fold-resistance legend to the Figure 2 P4 mean-fold-resistance legend.