Rst_Hydrolase-3Tm system

Edge evidence

• Rst_Hydrolase-3Tm locus contributes to T7 phage resistance RO:0002326

  Rousset et al. linked a HAD-like hydrolase plus transmembrane-protein locus to T7 restriction, and DefenseFinder models Rst_Hydrolase-3Tm as a two-profile Rst_Hydrolase-Tm system.

  • DOI:10.1016/j.chom.2022.02.018 two systems that specifically inhibit the growth of phage T7: an ATP-dependent helicase associated with a DUF2290 protein and a HAD-like hydrolase associated with a transmembrane protein Rousset et al. experimentally linked a HAD-like hydrolase plus transmembrane-protein pair to specific inhibition of phage T7 growth.
  • https://gitlab.pasteur.fr/mdm-lab/wiki/-/raw/223cf269/content/3.defense-systems/rst_hydrolase-3tm.md The Rst_Hydrolase-3Tm is composed of 2 proteins: Hydrolase and Hydrolase-Tm. The pinned DefenseFinder wiki page names Rst_Hydrolase-3Tm as a two-component Hydrolase/Hydrolase-Tm system.
  • https://raw.githubusercontent.com/mdmparis/defense-finder-models/afb0e5a8b466be53586b13266f5d38d98c3ac268/DefenseFinder_rules.tsv Rst_Hydrolase-3Tm Rst_Hydrolase-Tm 2 2 Rst_Hydrolase-Tm__Hydrolase, Rst_Hydrolase-Tm__Hydrolase-Tm The DefenseFinder rules table maps Rst_Hydrolase-3Tm to a two-profile Rst_Hydrolase-Tm model requiring Hydrolase and Hydrolase-Tm profiles.
  • https://raw.githubusercontent.com/mdmparis/defense-finder-models/afb0e5a8b466be53586b13266f5d38d98c3ac268/Liste_hmm_system.md | Rst_Hydrolase-Tm__Hydrolase | Rst_Hydrolase-Tm__Hydrolase | Rst_Hydrolase-Tm | Custom | 20 | | Rst_Hydrolase-Tm__Hydrolase-Tm | Rst_Hydrolase-Tm__Hydrolase-Tm | Rst_Hydrolase-Tm | Custom | 20 | The DefenseFinder HMM inventory records Hydrolase and Hydrolase-Tm profiles under the normalized Rst_Hydrolase-Tm system namespace.
• T7 phage resistance confers Rst_Hydrolase-3Tm system METPO:2007700

  Rst_Hydrolase-3Tm-linked T7 resistance realizes the Rst_Hydrolase-3Tm system trait.

  • DOI:10.1016/j.chom.2022.02.018 two systems that specifically inhibit the growth of phage T7: an ATP-dependent helicase associated with a DUF2290 protein and a HAD-like hydrolase associated with a transmembrane protein Rousset et al. experimentally linked a HAD-like hydrolase plus transmembrane-protein pair to specific inhibition of phage T7 growth.
  • https://gitlab.pasteur.fr/mdm-lab/wiki/-/raw/223cf269/content/3.defense-systems/rst_hydrolase-3tm.md Rousset_2022[<a href='https://doi.org/10.1016/j.chom.2022.02.018'>Rousset et al., 2022</a>] --> Origin_0 Origin_0[Escherichia coli P4 loci <a href='https://ncbi.nlm.nih.gov/protein/WP_000754434.1'>WP_000754434.1</a>, <a href='https://ncbi.nlm.nih.gov/protein/WP_001401335.1'>WP_001401335.1</a>] --> Expressed_0[Escherichia coli] Expressed_0[Escherichia coli] ----> T7 The DefenseFinder experimental-validation diagram links Rousset et al. to an Escherichia coli P4-locus source expressed in Escherichia coli against T7.
  • https://gitlab.pasteur.fr/mdm-lab/wiki/-/raw/223cf269/content/3.defense-systems/rst_hydrolase-3tm.md subgraph Title4[Protects against] T7 The DefenseFinder experimental-validation diagram lists T7 in the Rst_Hydrolase-3Tm protects-against subgraph.
• Rst_Hydrolase-3Tm system is a phage defense system rdfs:subClassOf

  Rst_Hydrolase-3Tm system possession is a phage-defense-system trait.

  • DOI:10.1016/j.chom.2022.02.018 Phage resistance heatmap of the validated defense systems shows the mean fold resistance of three independent replicates against a panel of eight phages The Figure 2 legend frames the cloned P4-hotspot hits, including the hydrolase plus transmembrane-protein system, as validated defense systems in a replicated phage-resistance heatmap.
  • https://raw.githubusercontent.com/mdmparis/defense-finder-models/afb0e5a8b466be53586b13266f5d38d98c3ac268/List_system_article.md Rst_Hydrolase-3Tm | 10\.1101/2021\.01\.21\.427644 | Prophage-encoded hotspots of bacterial immune systems The DefenseFinder article registry maps the named Rst_Hydrolase-3Tm system to the Rousset et al. preprint.