Rst_RT-nitrilase-Tm system

Edge evidence

• Rst_RT-nitrilase-Tm locus contributes to AL505_P2 phage resistance RO:0002326

  Rousset et al. linked a group-5 reverse transcriptase/nitrilase plus transmembrane effector locus to phage resistance, and DefenseFinder models Rst_RT-nitrilase-Tm as a two-profile Rst_RT-Tm system.

  • DOI:10.1016/j.chom.2022.02.018 with a C-terminal nitrilase domain (Table S3) that is associated with a transmembrane effector Rousset et al. described a validated P4-hotspot system containing a group-5 reverse transcriptase with a C-terminal nitrilase domain associated with a transmembrane effector.
  • https://gitlab.pasteur.fr/mdm-lab/wiki/-/raw/ee7647d8/content/3.defense-systems/rst_rt-nitrilase-tm.md The first protein is a reverse transcriptase (RT) fussed with C-N hydrolase domain (nitrilase); the second protein is transmembrane protein :ref{doi=10.1093/nar/gkac467}. The pinned DefenseFinder wiki page describes the reverse-transcriptase/nitrilase plus transmembrane-protein architecture.
  • https://gitlab.pasteur.fr/mdm-lab/wiki/-/raw/ee7647d8/content/3.defense-systems/rst_rt-nitrilase-tm.md The Rst_RT-nitrilase-Tm is composed of 2 proteins: RT and RT-Tm. The pinned DefenseFinder wiki page maps the Rst-prefixed name to a two-component RT/RT-Tm structure.
  • https://raw.githubusercontent.com/mdmparis/defense-finder-models/afb0e5a8b466be53586b13266f5d38d98c3ac268/DefenseFinder_rules.tsv Rst_RT-nitrilase-Tm Rst_RT-Tm 2 2 Rst_RT-Tm__RT, Rst_RT-Tm__RT-Tm The DefenseFinder rules table maps Rst_RT-nitrilase-Tm to a two-profile Rst_RT-Tm model requiring RT and RT-Tm profiles.
  • https://raw.githubusercontent.com/mdmparis/defense-finder-models/afb0e5a8b466be53586b13266f5d38d98c3ac268/Liste_hmm_system.md | Rst_RT-Tm__RT | Rst_RT-Tm__RT | Rst_RT-Tm | Custom | 500 | | Rst_RT-Tm__RT2 | | Rst_RT-Tm | Custom | 100 | | Rst_RT-Tm__RT-Tm | Rst_RT-Tm__RT-Tm | Rst_RT-Tm | Custom | 100 | The DefenseFinder HMM inventory records the RT, alternate RT2, and RT-Tm profiles under the normalized Rst_RT-Tm system namespace.
• AL505_P2 phage resistance confers Rst_RT-nitrilase-Tm system METPO:2007700

  Rst_RT-nitrilase-Tm-linked AL505_P2 resistance realizes the Rst_RT-nitrilase-Tm system trait.

  • DOI:10.1016/j.chom.2022.02.018 Phage resistance heatmap of the validated defense systems shows the mean fold resistance of three independent replicates against a panel of eight phages The Figure 2 legend frames the cloned P4-hotspot hits, including the RT-nitrilase plus one-TM system, as validated defense systems in a replicated phage-resistance heatmap.
  • https://gitlab.pasteur.fr/mdm-lab/wiki/-/raw/ee7647d8/content/3.defense-systems/rst_rt-nitrilase-tm.md RT-nitrilase-Tm (also named UG5-large) is a two-gene defense system. It was discovered from P4-like satellites in *E. coli* genomes :ref{doi=10.1016/j.chom.2022.02.018} :ref{doi=10.1093/nar/gkac467}. Its antiphage activity was shown in *E. coli* against phage AL505_P2 (Myoviridae). The pinned DefenseFinder wiki page names RT-nitrilase-Tm, also called UG5-large, as a two-gene system and records AL505_P2 antiphage activity in Escherichia coli.
  • https://gitlab.pasteur.fr/mdm-lab/wiki/-/raw/ee7647d8/content/3.defense-systems/rst_rt-nitrilase-tm.md Expressed_0[Escherichia coli] ----> Al505_P2 The DefenseFinder experimental-validation diagram links Escherichia coli expression of the Rst_RT-nitrilase-Tm system to protection against AL505_P2.
• Rst_RT-nitrilase-Tm system is a phage defense system rdfs:subClassOf

  Rst_RT-nitrilase-Tm system possession is a phage-defense-system trait.

  • DOI:10.1016/j.chom.2022.02.018 Phage resistance heatmap of the validated defense systems shows the mean fold resistance of three independent replicates against a panel of eight phages The Figure 2 legend frames the cloned P4-hotspot hits, including the RT-nitrilase plus one-TM system, as validated defense systems in a replicated phage-resistance heatmap.
  • https://raw.githubusercontent.com/mdmparis/defense-finder-models/afb0e5a8b466be53586b13266f5d38d98c3ac268/List_system_article.md Rst_RT-nitrilase-Tm | 10\.1101/2021\.01\.21\.427644 | Prophage-encoded hotspots of bacterial immune systems The DefenseFinder article registry maps the named Rst_RT-nitrilase-Tm system to the Rousset et al. preprint.