LYSP100-associated nuclear domain

GO:0016606 · Other · Other · Proposed

A nuclear body that is enriched in the lymphoid cell-specific protein LYSp100B; LANDs are globular, electron-dense structures and are morphologically distinct from the annular structures characteristic of PML bodies. [GO:0016606]

Synonyms: LANDs

Part of: nuclear lumen (GO:0031981)

UniProt location names: UniProt Consortium, CC BY 4.0. Reference label and route sources.

Components

Component	Type	Part of / acts on	Grounding	Genes	Stoichiometry	Essential	Role
LYSp100B/Sp140	Protein	part of	Reviewed label only (reviewed name; no accession asserted)	SP140		UNKNOWN	Lymphoid-cell SP100 homolog that concentrates in LANDs and can be redistributed by the Bright/ARID3A transcription factor. `PMID:8695863` Dent et al. 1996 cloned human LYSP100 and localized LYSP100 protein to LAND nuclear dots that were mostly nonoverlapping with PML nuclear bodies. `DOI:10.1093/emboj/19.15.4123` Zong, Das and Tucker 2000 analyzed the LYSp100B/Sp140 homolog of Sp100 and its redistribution from LANDs after interaction with Bright.

Taxonomic distribution

NCBITaxon:9606 Homo sapiens — VARIABLE: LANDs were defined around human lymphoid-cell LYSP100, with HeLa transfection used to show that LYSP100B can generate LAND-like nuclear dots. [PMID:8695863]

Canonical examples

NCBITaxon:9606 Homo sapiens — Human LYSP100/LYSp100B was cloned and localized to globular, electron-dense LAND nuclear domains distinct from PML nuclear bodies. [PMID:8695863]

Functions

Bright-dependent immunoglobulin heavy-chain enhancer regulation — LYSp100B/Sp140 can interact with the Bright/ARID3A matrix-attachment region-binding transcription factor and is redistributed from LANDs while stimulating Bright transactivation through the immunoglobulin heavy-chain intronic enhancer.
- DOI:10.1093/emboj/19.15.4123 Zong, Das and Tucker 2000 connected LYSp100B, Bright and LAND redistribution with matrix-attachment-region-dependent IgH enhancer transcription in lymphoid cells.

Mechanism graphs

LYSp100B marks LANDs and is redistributed by Bright (REGULATION)

LYSp100B/Sp140 concentrates in globular LAND nuclear domains distinct from PML bodies and can redistribute from LANDs when it interacts with the Bright/ARID3A immunoglobulin transcription factor.

Subject	Predicate	Object	Evidence
LYSp100B/Sp140	localizes to	LYSP100-associated nuclear domain	`PMID:8695863` Dent et al. 1996 used immunofluorescence and immunoelectron microscopy to localize LYSP100 to LANDs.
LYSP100-associated nuclear domain	is distinct from	PML body	`GO:0016606` GO:0016606 defines LANDs as morphologically distinct from PML bodies. `PMID:8695863` Dent et al. 1996 described the morphological and biochemical distinction between LANDs and PML bodies.
Bright/ARID3A	redistributes	LYSp100B/Sp140	`DOI:10.1093/emboj/19.15.4123` Zong, Das and Tucker 2000 reported Bright-dependent redistribution of LYSp100B from its associated LANDs.
LYSp100B/Sp140	modulates	IgH enhancer transactivation	`DOI:10.1093/emboj/19.15.4123` Zong, Das and Tucker 2000 found that LYSp100B strongly stimulated Bright transactivation through the E mu enhancer.

Discussions

CURATION_TODO (OPEN): Resolve exact LAND protein-family boundaries and the relationship between LYSp100B-only LANDs, PML-positive LANDs, SP100 and Bright/ARID3A. This first GO:0016606 record captures LYSp100B/Sp140 as the defining LAND-enriched protein while leaving the subset of LANDs that contain PML, the weaker LYSp100B-Bright interaction, exact SP140 isoform boundaries and the stable-resident status of PML, SP100 and Bright to a separate curation pass.

Evidence

GO:0016606 Gene Ontology cellular-component term for LYSP100-associated nuclear domains and the exact LANDs synonym.
GO:0016604 Gene Ontology cellular-component parent term for nuclear body.
GO:0031981 Gene Ontology cellular-component term for the nuclear lumen; LANDs are LYSP100-associated nuclear bodies.
PMID:8695863 Dent et al. 1996, LYSP100-associated nuclear domains (LANDs): description of a new class of subnuclear structures and their relationship to PML nuclear bodies, Blood.
DOI:10.1093/emboj/19.15.4123 Zong, Das and Tucker 2000, Regulation of matrix attachment region-dependent, lymphocyte-restricted transcription through differential localization within promyelocytic leukemia nuclear bodies, EMBO Journal.

Curation history

2026-09-28T05:26:18Z codex · CREATED_RECORD — Scaffolded by scripts/new_record.py
2026-09-28T05:27:30Z codex · EXPANDED_RECORD — Added GO LAND synonymy, nuclear-body parentage, nuclear-lumen parthood, LYSp100B/Sp140 component curation, human LAND scope, Bright-dependent IgH enhancer function evidence, a nonmechanistic LYSp100B redistribution graph and a LAND/PML/Bright boundary TODO.

Source: data/structures/other/lysp100_associated_nuclear_domain.yaml