bhi_medium_with_additional_glucose

CultureMech:001316 bacterial COMPLEX LIQUID
Original name: BHI MEDIUM WITH ADDITIONAL GLUCOSE

Overview

External ontology
mediadive.medium:215b (source link unavailable) — BHI MEDIUM WITH ADDITIONAL GLUCOSE
Applications
  • Microbial cultivation
Synonyms
  • {'name': 'bhi_medium', 'source': 'KOMODO', 'source_id': 'komodo.medium:215', 'original_category': 'bacterial'}
  • {'name': 'bhi_medium_modified', 'source': 'KOMODO', 'source_id': 'komodo.medium:215a', 'original_category': 'bacterial'}
  • {'name': 'bhi_medium_for_strict_anaerobes', 'source': 'KOMODO', 'source_id': 'komodo.medium:215c', 'original_category': 'bacterial'}
  • {'name': '1245', 'source': 'unknown', 'original_category': 'bacterial'}
  • {'name': 'brain_heart_infusion_broth', 'source': 'TOGO', 'source_id': 'TOGO:M2511', 'original_category': 'bacterial'}
  • {'name': 'brain_heart_infusion_agar', 'source': 'TOGO', 'source_id': 'TOGO:M2971', 'original_category': 'bacterial'}
  • {'name': 'bhi_agar', 'source': 'TOGO', 'source_id': 'TOGO:M2982', 'original_category': 'bacterial'}
  • {'name': 'bhi_broth', 'source': 'TOGO', 'source_id': 'TOGO:M2485', 'original_category': 'bacterial'}
  • {'name': 'bhi_medium', 'source': 'MediaDive', 'source_id': 'mediadive.medium:215', 'original_category': 'bacterial'}
  • {'name': 'brain_heart_agar_bhi_luqiao_beijing', 'source': 'TOGO', 'source_id': 'TOGO:M2898', 'original_category': 'bacterial'}
  • {'name': 'brain_heart_infusion_bhi_broth', 'source': 'TOGO', 'source_id': 'TOGO:M2507', 'original_category': 'bacterial'}
  • {'name': 'brain_heart_infusion_bhi_difco_agar', 'source': 'TOGO', 'source_id': 'TOGO:M2956', 'original_category': 'bacterial'}
  • {'name': 'brain_heart_infusion_broth_bhi_oxoid', 'source': 'TOGO', 'source_id': 'TOGO:M2905', 'original_category': 'bacterial'}
  • {'name': 'brain_heart_infusion_broth_himedia', 'source': 'TOGO', 'source_id': 'TOGO:M2881', 'original_category': 'bacterial'}
  • {'name': 'brain_heart_infusion_media', 'source': 'TOGO', 'source_id': 'TOGO:M2837', 'original_category': 'bacterial'}
  • {'name': 'brain_heart_infusion_with_5_nacl', 'source': 'MediaDive', 'source_id': 'mediadive.medium:J72', 'original_category': 'bacterial'}
  • {'name': 'for_dsm_10643', 'source': 'KOMODO', 'source_id': 'komodo.medium:215c.1', 'original_category': 'bacterial'}
  • {'name': 'for_dsm_19851', 'source': 'KOMODO', 'source_id': 'komodo.medium:215c.3', 'original_category': 'bacterial'}
  • {'name': 'medium_215_modified_for_dsm_10548', 'source': 'KOMODO', 'source_id': 'komodo.medium:215_10548', 'original_category': 'bacterial'}
  • {'name': 'medium_215_modified_for_dsm_10599', 'source': 'KOMODO', 'source_id': 'komodo.medium:215_10599', 'original_category': 'bacterial'}
  • {'name': 'medium_215_modified_for_dsm_13140', 'source': 'KOMODO', 'source_id': 'komodo.medium:215_13140', 'original_category': 'bacterial'}
  • {'name': 'medium_215_modified_for_dsm_13191', 'source': 'KOMODO', 'source_id': 'komodo.medium:215_13191', 'original_category': 'bacterial'}
  • {'name': 'medium_215_modified_for_dsm_13666', 'source': 'KOMODO', 'source_id': 'komodo.medium:215_13666', 'original_category': 'bacterial'}
  • {'name': 'medium_215_modified_for_dsm_13667', 'source': 'KOMODO', 'source_id': 'komodo.medium:215_13667', 'original_category': 'bacterial'}
  • {'name': 'medium_215_modified_for_dsm_21511', 'source': 'KOMODO', 'source_id': 'komodo.medium:215_21511', 'original_category': 'bacterial'}
  • {'name': 'medium_215_modified_for_dsm_22834', 'source': 'KOMODO', 'source_id': 'komodo.medium:215_22834', 'original_category': 'bacterial'}
  • {'name': 'medium_215_modified_for_dsm_23150', 'source': 'KOMODO', 'source_id': 'komodo.medium:215_23150', 'original_category': 'bacterial'}
  • {'name': 'medium_215_modified_for_dsm_25050', 'source': 'KOMODO', 'source_id': 'komodo.medium:215_25050', 'original_category': 'bacterial'}
  • {'name': 'medium_215_modified_for_dsm_8629', 'source': 'KOMODO', 'source_id': 'komodo.medium:215_8629', 'original_category': 'bacterial'}
  • {'name': 'medium_215b_modified_for_dsm_41834', 'source': 'KOMODO', 'source_id': 'komodo.medium:215b_41834', 'original_category': 'bacterial'}
  • {'name': 'modified_bhi', 'source': 'MediaDive', 'source_id': 'mediadive.medium:J290', 'original_category': 'bacterial'}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium215b.pdf Commercial Product: Brain Heart Infusion Supplier: Difco/BD Catalog: 237500 Source: https://microbenotes.com/brain-heart-infusion-bhi-agar/ Accessed: 2026-03-28 Constituent composition researched from product specification.

Composition

flowchart LR
    CultureMech_001316["bhi_medium_with_additional_glucose"]
    ing0_Calf_brains["Calf brains"]
    CultureMech_001316 --> ing0_Calf_brains
    ing1_Beef_heart["Beef heart"]
    CultureMech_001316 --> ing1_Beef_heart
    ing2_Proteose_peptone["Proteose peptone"]
    CultureMech_001316 --> ing2_Proteose_peptone
    ing3_Dextrose["Dextrose"]
    CultureMech_001316 --> ing3_Dextrose
    CHEBI_17634["CHEBI:17634"]
    ing3_Dextrose -.-> CHEBI_17634
    ing4_Sodium_chloride["Sodium chloride"]
    CultureMech_001316 --> ing4_Sodium_chloride
    CHEBI_26710["CHEBI:26710"]
    ing4_Sodium_chloride -.-> CHEBI_26710
    ing5_Disodium_phosphate["Disodium phosphate"]
    CultureMech_001316 --> ing5_Disodium_phosphate
    CHEBI_34683["CHEBI:34683"]
    ing5_Disodium_phosphate -.-> CHEBI_34683
    ing6_Glucose["Glucose"]
    CultureMech_001316 --> ing6_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing6_Glucose -.-> CHEBI_17234

Ingredients (7)

# Ingredient Concentration Ontology Notes
1 Calf brains
Difco/BD Brain Heart Infusion 237500
variable VARIABLE UBERON:0000955
brain
 infusion from 200 g/L; Tissue infusion - source material is calf brain tissue; Constituent of Brain Heart Infusion commercial product
2 Beef heart
Difco/BD Brain Heart Infusion 237500
variable VARIABLE UBERON:0000948
heart
 infusion from 250 g/L; Tissue infusion - source material is beef/ox heart tissue; Constituent of Brain Heart Infusion commercial product
3 Proteose peptone
Difco/BD Brain Heart Infusion 237500
10.0 G_PER_L MICRO:0000180 (source link unavailable)
proteose peptone
 Enzymatic digest of animal protein providing amino acids and peptides; Constituent of Brain Heart Infusion commercial product
4 Dextrose
C6H12O6 180.156 g/mol
Difco/BD Brain Heart Infusion 237500
2.0 G_PER_L CHEBI:17634
D-glucose
 Carbon/energy source; Constituent of Brain Heart Infusion commercial product
5 Sodium chloride
Cl.Na 58.443 g/mol
Difco/BD Brain Heart Infusion 237500
5.0 G_PER_L CHEBI:26710
sodium chloride
 Maintains osmotic equilibrium; Constituent of Brain Heart Infusion commercial product
6 Disodium phosphate
HO4P.2Na 141.958 g/mol
Difco/BD Brain Heart Infusion 237500
2.5 G_PER_L CHEBI:34683
disodium hydrogenphosphate
 pH buffer (final pH 7.4 ± 0.2); Constituent of Brain Heart Infusion commercial product
7 Glucose
C6H12O6 180.156 g/mol
5 G_PER_L CHEBI:17234
glucose

Target organisms

No target organisms are recorded.

Provenance

Merged from 33 source records.

TimestampCuratorActionNotes
2026-01-26T17:53:10.537874Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 215b
2026-03-13T06:19:03.175911Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001316
2026-03-17T06:29:49.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:15.069591+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "BHI MEDIUM WITH ADDITIONAL GLUCOSE" to "bhi_medium_with_additional_glucose" for consistent matching
2026-06-05T07:40:50.918334+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-08-06T03:04:45.215905+00:00 recipe-merger Merged 33 duplicate recipes into canonical record Sources: KOMODO_215_BHI_medium, KOMODO_215a_BHI_medium_modified, KOMODO_215b_BHI_medium_WITH_ADDITIONAL_GLUCOSE, KOMODO_215c_BHI_medium_FOR_STRICT_ANAEROBES, NBRC_1245, TOGO_M2511_brain_heart_infusion_broth, TOGO_M2971_Brain_Heart_Infusion_Agar, bhi_agar, bhi_broth, bhi_medium, bhi_medium_with_additional_glucose, brain_heart_agar_bhi_luqiao_beijing, brain_heart_infusion_bhi_broth, brain_heart_infusion_bhi_difco_agar, brain_heart_infusion_broth_bhi_oxoid, brain_heart_infusion_broth_himedia, brain_heart_infusion_media, brain_heart_infusion_with_5_nacl, for_dsm_10643, for_dsm_19851, medium_215_modified_for_dsm_10548, medium_215_modified_for_dsm_10599, medium_215_modified_for_dsm_13140, medium_215_modified_for_dsm_13191, medium_215_modified_for_dsm_13666, medium_215_modified_for_dsm_13667, medium_215_modified_for_dsm_21511, medium_215_modified_for_dsm_22834, medium_215_modified_for_dsm_23150, medium_215_modified_for_dsm_25050, medium_215_modified_for_dsm_8629, medium_215b_modified_for_dsm_41834, modified_bhi
2026-08-06T03:04:45.216102+00:00 merge_recipes.py MERGED_RECIPES merged 33 source recipe(s) on fingerprint c9f479eb46e904ba5f4f951ddac3fa23ee2c06f09ce437f640c578c867514d28