r3_a_medium

CultureMech:003863 bacterial COMPLEX SOLID_AGAR
Original name: R3 A medium

Overview

External ontology
komodo.medium:1153 (source link unavailable) — R3 A medium
Applications
  • Microbial cultivation
Synonyms
  • {'name': '1_10_r2a_medium', 'source': 'MediaDive', 'source_id': 'mediadive.medium:1578', 'original_category': 'bacterial'}
  • {'name': 'half_strength_r2a_medium_in_75_seawater', 'source': 'KOMODO', 'source_id': 'komodo.medium:1320', 'original_category': 'bacterial'}
  • {'name': 'limnobacter_medium', 'source': 'KOMODO', 'source_id': 'komodo.medium:919', 'original_category': 'bacterial'}
  • {'name': 'double_concentrated_830_0_06_sodium_pyruvate', 'source': 'MediaDive', 'source_id': 'mediadive.medium:1641', 'original_category': 'bacterial'}
  • {'name': 'half_strength_r2a', 'source': 'MediaDive', 'source_id': 'mediadive.medium:1492', 'original_category': 'bacterial'}
  • {'name': 'half_strength_r2a_medium_in_75_seawater', 'source': 'MediaDive', 'source_id': 'mediadive.medium:1320', 'original_category': 'bacterial'}
  • {'name': 'limnobacter_medium', 'source': 'MediaDive', 'source_id': 'mediadive.medium:919', 'original_category': 'bacterial'}
  • {'name': 'r2a_medium', 'source': 'MediaDive', 'source_id': 'mediadive.medium:830', 'original_category': 'bacterial'}
  • {'name': 'reactivation_with_liquid_medium_830', 'source': 'MediaDive', 'source_id': 'mediadive.medium:830c', 'original_category': 'bacterial'}
pH
7.2
Notes
Source: KOMODO ModelSEED | ID: 1153 | DSMZ Medium: 1153 (mediadive.medium:1153) | Aerobic: No

Composition

flowchart LR
    CultureMech_003863["r3_a_medium"]
    ing0_Yeast_extract["Yeast extract"]
    CultureMech_003863 --> ing0_Yeast_extract
    ing1_Proteose_peptone["Proteose peptone"]
    CultureMech_003863 --> ing1_Proteose_peptone
    ing2_Casamino_acids["Casamino acids"]
    CultureMech_003863 --> ing2_Casamino_acids
    ing3_Glucose["Glucose"]
    CultureMech_003863 --> ing3_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing3_Glucose -.-> CHEBI_17234
    ing4_Starch["Starch"]
    CultureMech_003863 --> ing4_Starch
    CHEBI_28017["CHEBI:28017"]
    ing4_Starch -.-> CHEBI_28017
    ing5_Na_pyruvate["Na-pyruvate"]
    CultureMech_003863 --> ing5_Na_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing5_Na_pyruvate -.-> CHEBI_50144
    ing6_K2HPO4["K2HPO4"]
    CultureMech_003863 --> ing6_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing6_K2HPO4 -.-> CHEBI_131527
    ing7_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_003863 --> ing7_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing7_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing8_Sodium_pyruvate["Sodium pyruvate"]
    CultureMech_003863 --> ing8_Sodium_pyruvate
    ing8_Sodium_pyruvate -.-> CHEBI_50144
    ing9_Agar["Agar"]
    CultureMech_003863 --> ing9_Agar
    CHEBI_2509["CHEBI:2509"]
    ing9_Agar -.-> CHEBI_2509

Ingredients (10)

# Ingredient Concentration Ontology Notes
1 Yeast extract 1 G_PER_L
2 Proteose peptone 1 G_PER_L
3 Casamino acids 1 G_PER_L
4 Glucose
C6H12O6 180.156 g/mol
1 G_PER_L CHEBI:17234
glucose
5 Starch 1 G_PER_L CHEBI:28017
starch
6 Na-pyruvate
C3H3O3.Na 110.044 g/mol
0.6 G_PER_L CHEBI:50144
sodium pyruvate
7 K2HPO4
HO4P.2K 174.174 g/mol
0.6 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
8 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.1 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
9 Sodium pyruvate
C3H3O3.Na 110.044 g/mol
0.6 G_PER_L CHEBI:50144
sodium pyruvate
10 Agar 15 G_PER_L CHEBI:2509
agar

Target organisms

No target organisms are recorded.

Provenance

Merged from 11 source records.

TimestampCuratorActionNotes
2026-01-27T01:15:01.fZ komodo-web-import Imported from KOMODO web table Source: KOMODO, ID: 1153
2026-02-03T11:19:18.010568 dsmz-resolver-v1.0 Enriched with DSMZ composition Copied ingredients from DSMZ Medium 1153
2026-03-13T06:19:22.805061Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003863
2026-03-17T06:30:51.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:37.248319+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "R3 A medium" to "r3_a_medium" for consistent matching
2026-06-05T07:40:19.431446+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:27.577447+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (audit G21/G22) Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).
2026-06-10T07:46:43.643271+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:04:33.572553+00:00 recipe-merger Merged 11 duplicate recipes into canonical record Sources: 1_10_r2a_medium, KOMODO_1153_R3_A_medium, KOMODO_1320_HALF_STRENGTH_R2A_MEDIUM_IN_75_SEAWATER, KOMODO_919_LIMNOBACTER_medium, double_concentrated_830_0_06_sodium_pyruvate, half_strength_r2a, half_strength_r2a_medium_in_75_seawater, limnobacter_medium, r2a_medium, r3_a_medium, reactivation_with_liquid_medium_830
2026-08-06T03:04:33.572631+00:00 merge_recipes.py MERGED_RECIPES merged 11 source recipe(s) on fingerprint 4804452e9f68ca5e7c9e0f5171d35b2ab3c2e1280f6695fbc0f67ac430965fd1