lb_medium

Overview

External ontology

TOGO:M2314 — LB medium

Applications

• Microbial cultivation

Synonyms

• {'name': 'lb', 'source': 'unknown', 'original_category': 'algae'}
• {'name': '2_x_yt_medium', 'source': 'MediaDive', 'source_id': 'mediadive.medium:J321', 'original_category': 'bacterial'}
• {'name': 'lb_broth', 'source': 'TOGO', 'source_id': 'TOGO:M3227', 'original_category': 'bacterial'}
• {'name': 'LB', 'source': 'unknown', 'original_category': 'bacterial'}
• {'name': 'LB_0.1x', 'source': 'unknown', 'original_category': 'bacterial'}
• {'name': 'lb_agar', 'source': 'TOGO', 'source_id': 'TOGO:M2829', 'original_category': 'bacterial'}
• {'name': 'lb_broth', 'source': 'TOGO', 'source_id': 'TOGO:M3205', 'original_category': 'bacterial'}
• {'name': 'lb_broth_medium', 'source': 'TOGO', 'source_id': 'TOGO:M3223', 'original_category': 'bacterial'}
• {'name': 'LB_highSalt', 'source': 'unknown', 'original_category': 'bacterial'}
• {'name': 'lb_luria_bertani_broth_with_1_mm_dtt', 'source': 'MediaDive', 'source_id': 'mediadive.medium:J813', 'original_category': 'bacterial'}
• {'name': 'lb_luria_bertani_medium_lennox', 'source': 'MediaDive', 'source_id': 'mediadive.medium:J1236', 'original_category': 'bacterial'}
• {'name': 'lb_medium_difco', 'source': 'TOGO', 'source_id': 'TOGO:M2270', 'original_category': 'bacterial'}
• {'name': 'lb_medium_lennox', 'source': 'TOGO', 'source_id': 'TOGO:M2910', 'original_category': 'bacterial'}
• {'name': 'LB_Miller', 'source': 'unknown', 'original_category': 'bacterial'}
• {'name': 'lb_with_addition_of_1_wt_vol_nacl', 'source': 'TOGO', 'source_id': 'TOGO:M2268', 'original_category': 'bacterial'}
• {'name': 'tryptone_yeast_extract_medium', 'source': 'MediaDive', 'source_id': 'mediadive.medium:578', 'original_category': 'fungal'}
• {'name': 'tryptone_yeast_extract_medium_modified', 'source': 'MediaDive', 'source_id': 'mediadive.medium:1725', 'original_category': 'fungal'}

Notes

Source: https://togomedium.org/medium/M2314 Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification.

Composition

flowchart LR
    CultureMech_008901["lb_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_008901 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_Tryptone["Tryptone"]
    CultureMech_008901 --> ing1_Tryptone
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_008901 --> ing2_Yeast_extract
    ing3_Sodium_chloride["Sodium chloride"]
    CultureMech_008901 --> ing3_Sodium_chloride
    ing3_Sodium_chloride -.-> CHEBI_26710

Ingredients (4)

Target organisms and growth evidence

These are curated observations and conditions for named organisms; they do not imply universal growth suitability.

• Preferred term

  Escherichia coli K-12 MG1655

  Term

  Id

  NCBITaxon:511145

  Label

  Escherichia coli str. K-12 substr. MG1655

  Genome assembly id

  • GCA_000005845.2
  • GCF_000005845.2

  Strain

  K-12 MG1655

  Growth metrics

  • Measurement conditions

    LB medium batch culture; source monitored utilization of sugars, alcohols, and organic acids with transcriptional microarrays and associated nutrient utilization with the highest growth rate phase.

    Evidence

    • Reference

      PMID:16628448

      Supports

      SUPPORT

      Snippet

      Utilization of these nutrients associated with the highest growth rate of the culture was followed by simultaneous induction of enzymes involved in assimilation of a large group of other carbon sources including D-mannose, melibiose, D-galactose, L-fucose, L-rhamnose, D-mannitol, amino sugars, trehalose, L-arabinose, glycerol, and lactate.

      Explanation

      The article title and abstract directly identify growth of Escherichia coli MG1655 on LB medium. The CultureMech record is LB Miller liquid medium, so this is modeled as exact-parent evidence rather than a variant.

    Is max attainment

    False

  Evidence

  • Reference

    PMID:16628448

    Supports

    SUPPORT

    Explanation

    The source title states growth of Escherichia coli MG1655 on LB medium and the abstract discusses the growth-rate phase of that culture.

  • Reference

    NCBI:GCF_000005845.2

    Supports

    SUPPORT

    Explanation

    NCBI lists RefSeq assembly GCF_000005845.2 and BioSample SAMN02604091 for Escherichia coli str. K-12 substr. MG1655.

Provenance

Merged from 19 source records.

• lb (normalized source unresolved)
• 2_x_yt_medium (CultureMech:002679)
• TOGO_M2314_LB_medium (CultureMech:008901)
• TOGO_M3196_LB_medium (CultureMech:009637)
• TOGO_M3227_LB_broth (CultureMech:009666)
• lb (normalized source unresolved)
• lb_0_1x (CultureMech:015465)
• lb_agar (CultureMech:009373)
• lb_broth (CultureMech:009646)
• lb_broth_medium (CultureMech:009663)
• lb_highsalt (CultureMech:015467)
• lb_luria_bertani_broth_with_1_mm_dtt (CultureMech:003157)
• lb_luria_bertani_medium_lennox (CultureMech:002403)
• lb_medium_difco (CultureMech:008857)
• lb_medium_lennox (CultureMech:009447)
• lb_miller (CultureMech:015468)
• lb_with_addition_of_1_wt_vol_nacl (CultureMech:008855)
• tryptone_yeast_extract_medium (CultureMech:010471)
• tryptone_yeast_extract_medium_modified (CultureMech:010460)