oxidase negative

Trait evidence (3)

• https://asm.org/protocols/oxidase-test-protocol

  The test can be used to distinguish Neisseria gonorrhoeae (oxidase positive) from Staphylococcus spp. and Streptococcus spp. (oxidase negative)

  The American Society for Microbiology protocol gives oxidase-negative Staphylococcus and Streptococcus spp. as contrasts to an oxidase-positive Neisseria gonorrhoeae result.

• DOI:10.5812/chbs-160199

  A positive result from the dry filter paper method is indicated by the appearance of a dark purple color within 5 to 10 seconds. A 'delayed positive' result is recognized when a color change occurs between 10 and 60 seconds, whereas a negative result is indicated by the paper remaining colorless or changing color after more than 60 seconds

  Hafezi and Khamar review the oxidase-test polarity and interpret a colorless dry filter paper, or a color change only after the cutoff, as a negative result.

• DOI:10.5812/chbs-160199

  Bacteria that are classified as oxidase-negative can be anaerobic, aerobic, or facultative. A negative oxidase result indicates merely the absence of cytochrome c oxidase, which is responsible for oxidizing the reactant, while these organisms may utilize alternative oxidases for electron transfer during respiration

  The review constrains interpretation of the negative assay outcome to cytochrome c oxidase under the oxidase-test readout, rather than absence of every terminal oxidase.