urease test

METPO:1007082 · CLASS · SEEDED

A biochemical test that detects urease enzyme activity by observing urea hydrolysis (typically via a pH-indicator color change). The test outcome (positive or negative) is captured by its child classes; this class itself does not assert urease activity.

Trait evidence (2)

DOI:10.5812/chbs-160199
Urea is classified as a diamide, and certain bacteria possessing the enzyme urease are capable of utilizing urea to generate ammonia, carbon dioxide, and water

Hafezi and Khamar describe the urease-test chemistry linking bacterial urease to urea conversion into alkaline products.
DOI:10.5812/chbs-160199
Both types contain a phenol red reagent that changes color with pH variations

The review describes phenol red as the pH indicator for both liquid and solid urease-test media.

Provenance

Identifier source: METPO (2026-06-12)
Author: Marcin Joachimiak
Definition source: http://purl.obolibrary.org/obo/OMP_0000222

Parent traits (1)

phenotype METPO:1000059

Children (1)

urease negative METPO:1007088

Synonyms (2)

urea hydrolysis test EXACT_SYNONYM · metpo.owl
urease activity test EXACT_SYNONYM · metpo.owl

kg-microbe context

No kg-microbe node embedding matched this record in the 2026-04-25 deepwalk.

Curation history

2026-09-11T14:52:53Z · SEEDED_FROM_METPO · seed_from_metpo
imported from data/raw/metpo.owl (CLASS)
2026-09-11T14:53:47Z · ENRICHED_FROM_LITERATURE · codex
Enriched the seeded METPO urease test with OMP definition provenance plus DOI-backed urea-hydrolysis and pH-indicator evidence after a repository-wide duplicate review covering ignored and hidden files.