pH range high

Edge evidence

• extreme-alkaliphile Na+/H+ antiport enables pH range high RO:0002327

  Robust Na+/H+ antiport enables growth at pH > 10.

  • DOI:10.1016/j.tim.2007.02.005 proton motive force Supports antiporter-driven proton re-import as the extreme-alkaliphile mechanism.
• pH range high is a pH range rdfs:subClassOf

  pH range high is a quantitative bin of the pH-range phenotype.

  • DOI:10.1038/nrmicro2549 alkaliphiles Supports the pH 10–14 range as a value within the pH-range distribution.
• Mrp Na+/H+ antiporter complex enables pH range high RO:0002327

  The hetero-oligomeric Mrp antiporter has an indispensable role in growth at high pH.

  • DOI:10.1038/nrmicro2549 The unusual hetero-oligomeric Mrp antiporter has an indispensable role at high pH.
• mrpA-G operon encodes Mrp Na+/H+ antiporter complex biolink:encodes

  The mrpA-G operon encodes the seven proteins forming the hetero-oligomeric Mrp complex.

  • DOI:10.1038/nrmicro2549 Mrp antiporters are encoded in operons containing genes for seven hydrophobic proteins; all required to form the hetero-oligomeric complex.
• transmembrane potential (delta-psi) drives Mrp Na+/H+ antiporter complex

  A large transmembrane potential drives electrogenic Na+/H+ antiport that imports H+.

  • DOI:10.1038/nrmicro2549 A large transmembrane potential is essential to drive electrogenic Na+/H+ and K+/H+ antiporters that import H+.
• Na+/solute symporters supplies cytoplasmic Na+

  Na+/solute symporters supply the cytoplasmic Na+ required to sustain antiport activity.

  • DOI:10.1038/nrmicro2549 The ongoing requirement for cytoplasmic Na+ to support high levels of alkaliphile antiport activity is met by numerous Na+/solute symporters.
• cytoplasmic Na+ enables Mrp Na+/H+ antiporter complex RO:0002327

  Cytoplasmic Na+ is the substrate that sustains high levels of alkaliphile antiport activity.

  • DOI:10.1038/nrmicro2549 Cytoplasmic Na+ is required to support high levels of alkaliphile antiport activity.
• F1Fo-ATP synthase contributes to alkaline pH homeostasis RO:0002326

  Proton uptake accompanying ATP synthesis contributes to alkaliphile pH homeostasis.

  • DOI:10.1038/nrmicro2549 The proton uptake that accompanies ATP synthesis by the F1Fo-ATP synthase contributes to alkaliphile pH homeostasis.
• alkaline pH homeostasis enables pH range high RO:0002327

  Maintenance of cytoplasmic pH homeostasis enables growth at strongly alkaline external pH.

  • DOI:10.1038/nrmicro2549 Alkaline pH homeostasis underlies extreme-alkaliphile growth above pH 10.
• external pH > 10.3 shifts speciation toward carbonate (CO3 2-) speciation

  External pH above ~10.3 shifts dissolved inorganic carbon speciation toward carbonate.

  • DOI:10.1128/AEM.01557-23 DIC speciation shifts strongly with pH, toward CO3= above ~10.3.