GC mid1

Edge evidence

• strong GC-biased gene conversion produces GC mid1 METPO:2000202

  Strong GC-biased gene conversion yields high genome-wide GC composition.

  • DOI:10.1186/1471-2148-10-374 GC-biased gene conversion Supports GC-biased gene conversion as the driver of high-GC composition.
• GC mid1 is a GC content rdfs:subClassOf

  GC mid1 is a quantitative bin of the GC-content phenotype.

  • DOI:10.1038/nrg2358 GC content Supports the >66.3% bin as a value within the GC-content distribution.
• NHEJ pathway (Ku, LigD) positively correlated with GC mid1

  Ku/NHEJ presence is strongly associated with elevated genomic GC content.

  • DOI:10.1371/journal.pgen.1008493 Strong association between Ku presence and elevated GC content; Pearson r=0.54 (p<2.2e-16). Correlational across prokaryotes.
• high double-strand break rate selects for GC mid1 METPO:2007401

  Higher DSB formation rate selects for increased GC content relative to genomic background.

  • DOI:10.1371/journal.pgen.1008493 Sites with higher DSB rates are under selection for increased GC content; proposed unifying driver of GC content across prokaryotes.
• DNA replication and repair (DRR) system composition strongly correlated with GC mid1

  DRR-system KEGG-ortholog composition explains a large fraction of genomic GC variance.

  • DOI:10.1128/spectrum.02145-22 Linear model using 217 DRR-related KEGG orthologs explains up to 88% of variance in genomic GC (multiple correlation 0.94).
• GC mid1 may increase NHEJ end-joining efficiency

  High GC may increase NHEJ repair efficiency by stabilizing short overhangs/microhomologies via extra hydrogen bonds.

  • DOI:10.1371/journal.pgen.1008493 High GC via increased hydrogen bonds may stabilize short overhang/microhomology pairing and increase NHEJ efficiency.