cellulolysis

Edge evidence

• cellulase systems enables cellulolysis RO:0002327

  Cellulase systems hydrolyze cellulose extracellularly.

  • DOI:10.1128/MMBR.66.3.506-577.2002 Lynd et al. review microbial cellulose utilization and the cellulase systems involved.
• cellulolysis produces glucose METPO:2000202

  Cellulose hydrolysis yields cellodextrins and glucose.

  • DOI:10.1016/j.cbpa.2015.10.018 Cragg et al. place cellulose deconstruction within lignocellulose degradation.
• endoglucanase degrades cellulose METPO:2000007

  Endoglucanase participates in concerted hydrolysis of cellulose.

  • DOI:10.1093/jambio/lxac002 Complete degradation of cellulose to glucose requires concerted action of cellobiohydrolases, endoglucanases and beta-glucosidases.
• cellobiohydrolase / exoglucanase degrades cellulose METPO:2000007

  Cellobiohydrolase/exoglucanase participates in concerted hydrolysis of cellulose.

  • DOI:10.1093/jambio/lxac002 Complete degradation of cellulose to glucose requires concerted action of cellobiohydrolases, endoglucanases and beta-glucosidases.
• beta-glucosidase hydrolyzes cellobiose METPO:2000013

  Beta-glucosidase hydrolyzes cellobiose in the downstream saccharification step.

  • DOI:10.1186/s13568-023-01658-0 Beta-glucosidase activity converting cellobiose into glucose.
• beta-glucosidase produces glucose METPO:2000202

  Beta-glucosidase releases glucose from cellobiose.

  • DOI:10.1186/s13568-023-01658-0 Beta-glucosidase activity converting cellobiose into glucose; product edge directly stated.
• cellulosome has backbone component scaffoldin

  Cellulosomes are multienzyme complexes built on scaffoldins.

  • DOI:10.1093/jambio/lxac002 They are multienzyme complexes built on scaffoldins.
• scaffoldin contains cohesin domain

  Scaffoldins bear cohesin modules.

  • DOI:10.3389/fmicb.2023.1288286 Scaffoldins are the non-catalytic backbone and bear modules called cohesin.
• dockerin domain binds to cohesin domain

  Dockerin-cohesin interaction assembles enzymes onto the scaffoldin.

  • DOI:10.1093/jambio/lxac002 Dockerin modules interacting with scaffoldin cohesin modules; canonical assembly interaction.
• carbon catabolite repression represses cellulolytic / lignocellulose-catabolic genes

  Carbon catabolite repression represses cellulolytic/lignocellulose-catabolic genes when glucose signals are high.

  • DOI:10.1093/jambio/lxac002 CcpA recognizing CRE sites to prevent transcription of lignocellulose-catabolic genes when glucose-derived signals are high.