cell width medium

Edge evidence

• standard growth conditions enables Rod-complex peptidoglycan synthesis RO:0002327

  Standard growth regimes support typical Rod-complex activity.

  • DOI:10.1146/annurev-cellbio-101011-155745 MreB-directed peptidoglycan synthesis Supports MreB-organized PG synthesis under standard conditions.
• Rod-complex peptidoglycan synthesis produces cell width medium METPO:2000202

  Rod-complex PG synthesis sustains a typical rod radius.

  • DOI:10.1038/nrmicro3088 rod-shape is maintained Supports lateral PG assembly as the producer of typical rod radius.
• cell width medium is a cell width rdfs:subClassOf

  Medium cell width is a quantitative bin of the cell-width phenotype.

  • DOI:10.1146/annurev-cellbio-101011-155745 MreB-directed peptidoglycan synthesis Supports medium width as a value within the regulated cell-width distribution.
• RodA-PBP2 complex positively regulates peptidoglycan polymerization and crosslinking

  Structural opening of the RodA-PBP2 complex couples and activates PG polymerization and crosslinking during elongation.

  • DOI:10.1038/s41467-023-39037-9 RodA-PBP2 undergoes dynamic exchange between closed and open states; structural opening couples activation of polymerization and crosslinking and is essential in vivo.
• peptidoglycan polymerization and crosslinking produces Rod-complex peptidoglycan synthesis METPO:2000202

  Elongasome polymerization/crosslinking constitutes the Rod-complex lateral peptidoglycan synthesis that sets rod radius.

  • DOI:10.1038/s41467-023-39037-9 Activation of polymerization and crosslinking is the elongation activity underlying Rod-complex lateral wall synthesis.
• MreC activates PBP2 RO:0002213

  MreC interacts with PBP2 and stimulates its peptidoglycan polymerization and crosslinking activity.

  • DOI:10.1002/mbo3.1385 MreC interacts with PBP2 and is thought to cause a structural change in PBP2 and stimulate peptidoglycan polymerization and crosslinking.
• MreD modulates activity of PBP2

  MreD modulates PBP2 activity; the balance between MreC and MreD determines PBP2 activity.

  • DOI:10.1002/mbo3.1385 The balance between MreC and MreD determines the activity of PBP2 (curated as modulatory).
• PBP2 enables Rod-complex peptidoglycan synthesis RO:0002327

  Activated PBP2 transpeptidation supports Rod-complex lateral peptidoglycan synthesis.

  • DOI:10.1002/mbo3.1385 PBP2 activity drives peptidoglycan polymerization and crosslinking of the Rod complex.
• class A penicillin-binding proteins (aPBPs) regulates cell width RO:0002211

  Class A PBPs regulate cell diameter; loss of aPBPs yields shorter, wider cells.

  • DOI:10.1038/s41467-023-41082-3 aPBPs regulate cell diameter; strains lacking aPBPs were moderately but significantly shorter and wider.