cell width

Edge evidence

• MreB cytoskeleton enables Rod-complex peptidoglycan synthesis RO:0002327

  MreB filaments organize Rod-complex peptidoglycan synthesis along the cylinder.

  • DOI:10.1146/annurev-cellbio-101011-155745 MreB-directed peptidoglycan synthesis Supports MreB as the organizer of lateral PG synthesis.
• Rod-complex peptidoglycan synthesis builds peptidoglycan cell wall biolink:produces

  Rod-complex peptidoglycan synthesis builds the lateral cylindrical wall.

  • DOI:10.1038/nrmicro3088 rod-shape is maintained Supports lateral PG assembly as the wall-building activity.
• peptidoglycan cell wall regulates cell width RO:0002211

  The peptidoglycan sacculus constrains cell width against turgor.

  • DOI:10.1038/nrmicro3088 rod-shape is maintained Supports the cell wall as the structural constraint on width.
• turgor pressure balances peptidoglycan cell wall

  Turgor pressure is mechanically balanced by the peptidoglycan wall, determining the steady-state radius.

  • DOI:10.1146/annurev-cellbio-101011-155745 MreB-directed peptidoglycan synthesis Supports wall mechanics as the determinant of steady-state cell width.
• class A PBP (aPBP) cell-wall synthesis system regulates cell width RO:0002211

  Rod and aPBP systems act oppositely to set cell diameter; MreB-directed lateral PG synthesis is the control point governing width.

  • DOI:10.3390/biom13050720 Review states Rod and aPBP systems "act oppositely to set B. subtilis cell diameter"; MreB-directed PG synthesis identified as the control point governing cell width.
• RodA-PBP2 core synthase enables Rod-complex peptidoglycan synthesis RO:0002327

  Activation/open state of the RodA-PBP2 core synthase couples PG polymerization and crosslinking required for elongation-based lateral wall synthesis.

  • DOI:10.1038/s41467-023-39037-9 RodA-PBP2 undergoes dynamic exchange between closed and open states; structural opening couples activation of polymerization and crosslinking and is essential in vivo.
• Rod-complex accessory proteins MreC/MreD/RodZ regulates RodA-PBP2 core synthase RO:0002211

  Rod-complex accessory components MreC/MreD/RodZ regulate RodA-PBP2 activity and thereby lateral wall synthesis affecting width.

  • DOI:10.1038/s41467-023-39037-9 Rod complex has accessory components MreCD and RodZ; genetic studies implicated MreC as a potential regulator of enzymatic activity.
• cell envelope bending rigidity regulates cell width RO:0002211

  Cell envelope bending rigidity constrains cell width homeostasis; width increases and saturates primarily under the control of envelope bending rigidity.

  • DOI:10.1101/2024.11.22.624946 Width increases and saturates primarily under the control of envelope bending rigidity, which constrains width homeostasis.