cell width large

Edge evidence

• rich growth conditions shifts Rod-complex peptidoglycan synthesis

  Rich growth conditions shift Rod-complex activity toward a larger radius set-point.

  • DOI:10.1126/science.aaa1313 cell size scales with growth rate Supports growth-rate-dependent enlargement of cell radius.
• Rod-complex peptidoglycan synthesis produces cell width large METPO:2000202

  Elevated Rod-complex activity yields a wide rod radius.

  • DOI:10.1146/annurev-cellbio-101011-155745 MreB-directed peptidoglycan synthesis Supports lateral PG synthesis as the producer of the wide cell radius.
• cell width large is a cell width rdfs:subClassOf

  Large cell width is a quantitative bin of the cell-width phenotype.

  • DOI:10.1146/annurev-cellbio-101011-155745 MreB-directed peptidoglycan synthesis Supports large width as a value within the regulated cell-width distribution.
• RodA-PBP2 allosteric activation positively regulates peptidoglycan polymerization and crosslinking

  Allosteric structural opening of RodA-PBP2 activates coupled PG polymerization and crosslinking.

  • DOI:10.1038/s41467-023-39037-9 "Structural opening couples the activation of polymerization and crosslinking and is essential in vivo."
• RodA-PBP2 elongasome complex mediates lateral sidewall peptidoglycan insertion

  Activated RodA-PBP2 elongasome synthesizes and inserts new PG at lateral wall sites.

  • DOI:10.1073/pnas.2215237120 "The elongasome... synthesizing and inserting new PG material at dispersed sites in the lateral cell wall."
• lateral sidewall peptidoglycan insertion contributes to Rod-complex peptidoglycan synthesis RO:0002326

  Lateral sidewall PG insertion is the Rod-complex-driven wall-elongation process that sets rod radius.

  • DOI:10.1146/annurev-cellbio-101011-155745 Lateral PG insertion is the MreB/Rod-complex activity governing rod radius.
• MreB cytoskeletal filaments orients circumferential peptidoglycan insertion

  MreB filaments orient peptidoglycan insertion perpendicular to the long cell axis, controlling rod radius.

  • DOI:10.1073/pnas.2301987120 "MreB filaments are thought to orient it orthogonally to the long cell axis" guiding PG insertion perpendicular to the long axis.
• Rod-complex circumferential rotation enables even peptidoglycan distribution RO:0002327

  Rotation of the Rod complex around the circumference enables evenly distributed PG insertion.

  • DOI:10.1002/mbo3.1385 "the Rod complex rotates perpendicularly to the long axis of the cell... allowing the insertion of peptidoglycan... in an evenly distributed manner."
• MreC-MreD balance regulates PBP2 transpeptidase activity RO:0002211

  The balance between MreC and MreD determines PBP2 synthase activity.

  • DOI:10.1002/mbo3.1385 "the balance between MreC and MreD determines the activity of PBP2"
• Lipid II required for peptidoglycan polymerization and crosslinking

  Lipid II is the substrate required for peptidoglycan polymerization by Rod/elongasome synthases.

  • DOI:10.3390/biom13050720 "GTases and TPases utilize the disaccharide pentapeptide of Lipid-II as a substrate for PG polymerization and synthesis of the PG layer."