cell width small

Edge evidence

• MreB cytoskeleton enables Rod-complex peptidoglycan synthesis RO:0002327

  MreB organizes lateral PG synthesis along the cylinder.

  • DOI:10.1146/annurev-cellbio-101011-155745 MreB-directed peptidoglycan synthesis Supports MreB as the lateral PG organizer.
• Rod-complex peptidoglycan synthesis produces cell width small METPO:2000202

  Rod-complex PG synthesis maintains a narrow cylinder radius.

  • DOI:10.1038/nrmicro3088 rod-shape is maintained Supports lateral PG assembly as the producer of the narrow rod radius.
• cell width small is a cell width rdfs:subClassOf

  Small cell width is a quantitative bin of the cell-width phenotype.

  • DOI:10.1146/annurev-cellbio-101011-155745 MreB-directed peptidoglycan synthesis Supports small width as a value within the regulated cell-width distribution.
• MreC activates RodA-PBP2 open conformation RO:0002213

  MreC favors the open, active conformation of the RodA-PBP2 synthase.

  • DOI:10.1038/s41467-023-39037-9 MreC favors the open, active PBP2 state; PBP2 suppressor alleles bypass defective MreC and increase polymerization activity (conserved Rod complex).
• RodA-PBP2 open conformation increases peptidoglycan polymerization and crosslinking RO:0002213

  The open RodA-PBP2 state couples activation of polymerization and crosslinking.

  • DOI:10.1038/s41467-023-39037-9 Structural opening couples activation of polymerization and crosslinking; open-state mutants show stronger polymerization (conserved SEDS-bPBP systems).
• peptidoglycan polymerization and crosslinking contributes to Rod-complex peptidoglycan synthesis RO:0002326

  PG polymerization and crosslinking constitute the Rod-complex synthetic output.

  • DOI:10.1038/s41467-023-39037-9 Open-state activation links RodA-PBP2 conformation to peptidoglycan synthetic output of the Rod complex.
• MreB cytoskeleton required for rod width/diameter control

  MreB is a key determinant of rod shape and steady-state diameter/width control.

  • DOI:10.1016/j.cell.2018.02.050 MreB is a key determinant of rod shape and diameter; perturbing MreB changes steady-state diameter and width control (broad, foundational).
• rod width/diameter control produces cell width small METPO:2000202

  Stable diameter control maintains the narrow rod radius set-point.

  • DOI:10.1016/j.cell.2018.02.050 Width/diameter control governs the steady-state rod radius producing the narrow width phenotype.
• Mg2+ inhibits peptidoglycan hydrolase activity RO:0002212

  Exogenous Mg2+ inhibits autolysins/cell-wall hydrolases.

  • DOI:10.1038/s41598-021-04294-5 Exogenous Mg2+ inhibits autolysins/CW hydrolases and compensates increased DL-endopeptidase activity (clearest environmental modifier edge).