pH optimum mid1

Edge evidence

• near-neutral external pH imposes minimal pH-homeostasis load

  Near-neutral external pH imposes minimal pH-homeostasis demand on the cell.

  • DOI:10.1038/nrmicro2549 cytoplasmic pH Supports near-neutral external pH as the regime with the smallest cytoplasmic-pH gradient to maintain.
• minimal pH-homeostasis load produces pH optimum mid1 METPO:2000202

  Minimal pH-homeostasis load yields a neutrophilic pH-optimum setpoint.

  • DOI:10.1038/nrmicro2549 pH homeostasis Supports a near-neutral optimum as the neutrophile outcome.
• pH optimum mid1 is a pH optimum rdfs:subClassOf

  pH optimum mid1 is a quantitative bin of the pH-optimum phenotype.

  • DOI:10.1016/j.tim.2007.02.005 proton motive force Supports a near-neutral optimum as a value within the pH-optimum distribution.
• F0F1-ATPase exports proton (H+) METPO:2000209

  The F0F1-ATPase actively exports protons to maintain cytoplasmic pH.

  • DOI:10.1371/journal.ppat.1011927 actively exporting protons via proton pumps such as the F0F1-ATPase; classic pH-homeostasis mechanism.
• amino-acid decarboxylation pathways consumes proton (H+) biolink:consumes

  Amino-acid decarboxylation pathways consume cytoplasmic protons, supporting pH homeostasis.

  • DOI:10.1371/journal.ppat.1011927 consume protons through amino-acid decarboxylation pathways (glutamate, lysine, arginine); general acid-resistance edge.
• glutamate decarboxylation / GABA pathway consumes proton (H+) biolink:consumes

  The glutamate decarboxylation / GABA pathway consumes intracellular H+.

  • DOI:10.1128/aem.00569-24 glutamate-based systems and the GABA metabolic pathway consume H+.
• ammonia (NH3) binds proton (H+)

  Ammonia binds free protons, buffering against acidification.

  • DOI:10.1128/aem.00569-24 generated NH3 bind with intracellular free H+ to form NH4+; direct acid-buffering chemistry, generalizable.
• ammonia (NH3) forms ammonium (NH4+) biolink:produces

  Ammonia combines with a proton to form ammonium, sequestering acid.

  • DOI:10.1128/aem.00569-24 generated NH3 bind with intracellular free H+ to form NH4+.
• F0F1-ATPase contributes to minimal pH-homeostasis load RO:0002326

  Proton export by the F0F1-ATPase keeps the cytoplasmic-pH gradient small near neutral, contributing to minimal homeostasis load.

  • DOI:10.1371/journal.ppat.1011927 Proton pumping via F0F1-ATPase maintains cytosolic pH; near-neutral external pH minimizes the pumping demand.