bioluminescence

Edge evidence

• luciferase enables bioluminescence RO:0002327

  Luciferase carries out the light-emitting reaction.

  • DOI:10.1016/j.csbj.2018.11.003 Brodl et al. review the bacterial luciferase mechanism.
• bioluminescence consumes molecular oxygen biolink:consumes

  Light emission consumes molecular oxygen.

  • DOI:10.1016/j.csbj.2018.11.003 Supports O2 as a luciferase co-substrate.
• bioluminescence enables bioluminescence RO:0002327

  The luciferase reaction realizes the bioluminescence trait.

  • DOI:10.1146/annurev.cellbio.21.012704.131001 Waters & Bassler support quorum-sensing control of luminescence.
• luciferase catalyzes long-chain aldehyde biolink:catalyzes

  Luciferase catalyzes the monooxygenation of long-chain aliphatic aldehydes.

  • DOI:10.1016/j.csbj.2018.11.003 The heterodimeric enzyme luciferase (LuxAB) catalyzes the monooxygenation of aliphatic aldehydes to the corresponding acids.
• reduced flavin mononucleotide (FMNH2) is substrate for bioluminescence

  FMNH2 is a required substrate consumed in the light-emitting luciferase reaction.

  • DOI:10.1016/j.csbj.2018.11.003 Long chain aldehydes, reduced flavin mononucleotide (FMNH2) and molecular oxygen (O2) are converted by the enzyme luciferase (LuxAB).
• long-chain aldehyde is substrate for bioluminescence

  Long-chain aldehyde is a required substrate of the light-emitting reaction.

  • DOI:10.1016/j.csbj.2018.11.003 Long chain aldehydes, reduced flavin mononucleotide (FMNH2) and molecular oxygen (O2) are converted by the enzyme luciferase (LuxAB).
• LuxG flavin reductase converts flavin mononucleotide (FMN)

  LuxG flavin reductase reduces free FMN to FMNH2 supplying the luciferase reaction.

  • DOI:10.1016/j.csbj.2018.11.003 LuxG converts free flavin (FMN) to reduced flavin (FMNH2); LuxG is a NAD(P)H-dependent flavin reductase.
• LuxG flavin reductase produces reduced flavin mononucleotide (FMNH2) METPO:2000202

  LuxG generates the reduced flavin substrate FMNH2.

  • DOI:10.1016/j.csbj.2018.11.003 LuxG converts free flavin (FMN) to reduced flavin (FMNH2).
• LuxCDE fatty acid reductase complex supplies long-chain aldehyde

  The LuxCDE fatty acid reductase complex supplies the long-chain aldehyde substrate.

  • DOI:10.1016/j.csbj.2018.11.003 To supply the long-chain aldehyde substrates to the luciferase, the proteins LuxC, LuxD, and LuxE constitute a fatty acid reductase complex.
• LuxI autoinducer synthase synthesizes 3-oxo-C6-HSL autoinducer

  LuxI autoinducer synthase produces the 3-oxo-C6-HSL signal.

  • DOI:10.1128/jb.00035-24 Identifying LuxI as the autoinducer synthase; the LuxI-produced 3-oxo-C6 HSL.
• 3-oxo-C6-HSL autoinducer binds LuxR transcription factor

  The 3-oxo-C6-HSL autoinducer binds the LuxR transcription factor.

  • DOI:10.1128/jb.00035-24 The LuxI-produced 3-oxo-C6 was shown to bind the N-terminal domain of the transcription factor LuxR.
• LuxR transcription factor activates expression of lux locus expression

  Autoinducer-bound LuxR activates expression of the lux locus.

  • DOI:10.1128/jb.00035-24 Bind the N-terminal domain of the transcription factor LuxR to activate the expression of the lux locus.
• lux locus expression produces luciferase METPO:2000202

  Expression of the lux locus produces the luciferase light-production machinery.

  • DOI:10.1128/jb.00035-24 Activation of the lux locus drives expression of the light-production machinery including luciferase.