piezotolerant

Edge evidence

• hydrostatic pressure regulates membrane lipid adaptation RO:0002211

  Hydrostatic pressure modulates membrane fluidity, prompting adaptive lipid composition.

  • DOI:10.3389/fmolb.2022.1058381 HHP adaptation involves unsaturated and branched-chain fatty acids.
• membrane lipid adaptation enables piezotolerant RO:0002327

  Membrane adaptation supports growth across the pressure range atmospheric ↔ high.

  • DOI:10.1099/ijsem.0.001671 Contrast with obligate piezophiles (growth limited to 80-140 MPa) defines piezotolerant as the atmospheric-capable phenotype.
• hydrostatic pressure decreases membrane fluidity RO:0002212

  High hydrostatic pressure compacts fatty acids, reducing membrane fluidity.

  • DOI:10.3390/microorganisms11071629 high hydrostatic pressure reduces membrane fluidity by compacting fatty acids
• membrane fluidity causes increase in unsaturated membrane fatty acids

  Reduced membrane fluidity is countered by increasing unsaturated fatty acid proportions.

  • DOI:10.3390/microorganisms11071629 organisms counter HHP by increasing the proportions of unsaturated fatty acids
• hydrostatic pressure increases abundance of branched-chain and unsaturated membrane fatty acids

  Membrane lipids with unsaturated and branched-chain fatty acids increase with rising pressure.

  • DOI:10.3389/fmolb.2022.1058381 abundance of membrane lipids containing unsaturated and branched-chain fatty acids increases with increasing HHP
• hydrostatic pressure induces transcription of heat-shock response genes (rpoH, rpoE, dnaK, groEL)

  Pressure shock transcriptionally upregulates key heat-shock genes.

  • DOI:10.3389/fmicb.2024.1470617 key heat shock genes rpoH, rpoE, dnaK, and groEL are transcriptionally upregulated following pressure shock
• hydrostatic pressure induces accumulation of piezolyte accumulation

  High pressure induces accumulation of piezolytes (compatible solutes).

  • DOI:10.3390/microorganisms11071629 piezolytes are molecules that accumulate in cells in response to high pressure (e.g., glutamate, betaine, beta-hydroxybutyrate)
• piezolyte accumulation protects protein protection under pressure

  Accumulated piezolytes protect proteins via preferential hydration under pressure.

  • DOI:10.3390/microorganisms11071629 compatible solutes act via preferential hydration to protect proteins