TraitMech

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catalase negative

METPO:1007084 · CLASS · SEEDED

Test-outcome phenotype where the catalase test yields a negative result (no bubbling on H2O2). The underlying enzyme-organism relation should additionally be asserted via <organism> METPO:2000303 'does not show activity of' GO:0004096 'catalase activity'.

Trait evidence (2)

  • DOI:10.1038/srep03081
    The enzyme-generated oxygen bubbles trapped by Triton X-100 were visualized as foam

    Iwase et al. tie the catalase assay signal to oxygen bubbles from hydrogen-peroxide decomposition, supporting no visible bubbling as a negative test outcome.

  • DOI:10.1128/iai.72.8.4512-4520.2004
    E. faecium is catalase negative, and the major mechanism to scavenge hydrogen peroxide is NADH peroxidase

    Moy et al. identify E. faecium as catalase-negative and contrast its NADH-peroxidase scavenging with catalase-bearing E. faecalis under heme-supplemented conditions.

Provenance

Identifier source
METPO (2026-06-12)
Author
Marcin Joachimiak
Definition source
DOI:10.1038/srep03081

Parent traits (1)

Synonyms (2)

  • catalase - EXACT_SYNONYM · metpo.owl
  • catalase test negative EXACT_SYNONYM · metpo.owl

kg-microbe context

No kg-microbe node embedding matched this record in the 2026-04-25 deepwalk.

Canonical examples (1)

Organisms cited as exemplars of this trait. Taxon ids are NCBITaxon and link out to the NCBI record.

Discussions and Knowledge Gaps (1)

Open questions attached to this trait. Seeded by just knowledge-gap-scan and curated; see the corpus-wide index.

Resolve a non-assay parent for negative catalase-test phenotypes before narrowing parent_traits below phenotype.

CURATION TODO RESOLVED catalase-negative-assay-parent-gap · raised by codex · 2026-09-11

Not yet attached to a section of this record — a curator sets attaches_to (e.g. causal_graphs#some_edge) so the gap shows beside the mechanism it concerns.

METPO:1007080 is now represented as the seeded catalase-test assay parent, so this negative assay-outcome phenotype can use its source superclass rather than a temporary direct phenotype parent.

Curation history

  1. · SEEDED_FROM_METPO · seed_from_metpo

    imported from data/raw/metpo.owl (CLASS)

  2. · ENRICHED_FROM_LITERATURE · codex

    Enriched the seeded METPO catalase negative test-outcome phenotype with DOI-backed assay and Enterococcus faecium evidence after a repository-wide duplicate review covering ignored and hidden files.

  3. · RESOLVED_PARENT_GAP · codex

    Reparented catalase negative below the newly curated METPO:1007080 catalase test assay parent and marked the temporary assay-parent discussion resolved.