catalase test

METPO:1007080 · CLASS · SEEDED

A biochemical test that detects catalase enzyme activity by exposing cells to hydrogen peroxide and observing for visible bubbling. The test outcome (positive or negative) is captured by its child classes; this class itself does not assert that the organism has catalase activity.

Trait evidence (2)

https://asm.org/protocols/catalase-test-protocol
The catalase test facilitates the detection of this enzyme in bacteria

The American Society for Microbiology catalase-test protocol describes the bacterial assay context for detecting catalase.
DOI:10.1038/srep03081
The enzyme-generated oxygen bubbles trapped by Triton X-100 were visualized as foam

Iwase et al. support the visible oxygen-bubble readout produced by catalase-mediated hydrogen-peroxide decomposition.

Provenance

Identifier source: METPO (2026-06-12)
Author: Marcin Joachimiak
Definition source: http://purl.obolibrary.org/obo/OMP_0000218

Parent traits (1)

phenotype METPO:1000059

Children (1)

catalase negative METPO:1007084

Synonyms (2)

catalase activity test EXACT_SYNONYM · metpo.owl
catalase assay EXACT_SYNONYM · metpo.owl

kg-microbe context

No kg-microbe node embedding matched this record in the 2026-04-25 deepwalk.

Curation history

2026-09-11T14:00:10Z · SEEDED_FROM_METPO · seed_from_metpo
imported from data/raw/metpo.owl (CLASS)
2026-09-11T14:03:00Z · ENRICHED_FROM_LITERATURE · codex
Enriched the seeded METPO catalase test with OMP definition provenance plus stable-URL and DOI-backed assay evidence after a repository-wide duplicate review covering ignored and hidden files.